Metabolic programming of Rhododendron chrysanthum leaves following exposure to UVB irradiation

Zhou, Xiaofu; Lyu, Jie; Sun, Li; Dong, Jiawei; Xu, Hongwei

doi:10.1071/fp20386

Cited by 21 publications

(17 citation statements)

References 46 publications

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“…The artificial radiation UV-B (Philips, Ultraviolet-B TL 20W/01 RS, Amsterdam, NY, The Netherlands) used in this experiment was the same as previously described [ 7 , 37 , 38 ]. In short, the leaves of R. chrysanthum were exposed to UVB artificial radiation for six replicates.…”

Section: Methodsmentioning

confidence: 99%

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

Sun

Liu

Chen

et al. 2022

Plants

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Under natural environmental conditions, excess UV-B stress can cause serious injuries to plants. However, domestication conditions may allow the plant to better cope with the upcoming UV-B stress. The leaves of Rhododendron chrysanthum are an evergreen plant that grows at low temperatures and high altitudes in the Changbai Mountains, where the harsh ecological environment gives it different UV resistance properties. Metabolites in R. chrysanthum have a significant impact on UV-B resistance, but there are few studies on the dynamics of their material composition and gene expression levels. We used a combination of gas chromatography time-of-flight mass spectrometry and transcriptomics to analyze domesticated and undomesticated R. chrysanthum under UV-B radiation. A total of 404 metabolites were identified, of which amino acids were significantly higher and carbohydrates were significantly lower in domesticated R. chrysanthum. Transcript profiles throughout R. chrysanthum under UV-B were constructed and analyzed, with an emphasis on sugar and amino acid metabolism. The transcript levels of genes associated with sucrose and starch metabolism during UV-B resistance in R. chrysanthum showed a consistent trend with metabolite content, while amino acid metabolism was the opposite. We used metabolomics and transcriptomics approaches to obtain dynamic changes in metabolite and gene levels during UV-B resistance in R. chrysanthum. These results will provide some insights to elucidate the molecular mechanisms of UV tolerance in plants.

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Section: Methodsmentioning

confidence: 99%

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

Sun

Liu

Chen

et al. 2022

Plants

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“…The chlorophyll fluorescence was obtained in order to analyze PSII quantum efficiency of plants by using an IMAGING PAM M-series (Walz, Effeltrich, Germany), and dark period of the samples was dark for 30 min before measurements. The maximum quantum yield of PSII (Fv/Fm), the effective quantum yield of PSII (φPSII), non-photochemical quenching coefficient (NPQ), and electron transport rate (ETR, μmol e –1 s –1 m –2 ) were calculated according to Zhou et al (2021) .…”

Section: Methodsmentioning

confidence: 99%

Improved Utilization of Nitrate Nitrogen Through Within-Leaf Nitrogen Allocation Trade-Offs in Leymus chinensis

et al. 2022

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The Sharply increasing atmospheric nitrogen (N) deposition may substantially impact the N availability and photosynthetic capacity of terrestrial plants. Determining the trade-off relationship between within-leaf N sources and allocation is therefore critical for understanding the photosynthetic response to nitrogen deposition in grassland ecosystems. We conducted field experiments to examine the effects of inorganic nitrogen addition (sole NH4+, sole NO3– and mixed NH4+/NO3–: 50%/50%) on N assimilation and allocation by Leymus chinensis. The leaf N allocated to the photosynthetic apparatus (NPSN) and chlorophyll content per unit area (Chlarea) were significantly positively correlated with the photosynthetic N-use efficiency (PNUE). The sole NO3– treatment significantly increased the plant leaf PNUE and biomass by increasing the photosynthetic N allocation and Chlarea. Under the NO3 treatment, L. chinensis plants devoted more N to their bioenergetics and light-harvesting systems to increase electron transfer. Plants reduced the cell wall N allocation or increased their soluble protein concentrations to balance growth and defense under the NO3 treatment. In the sole NH4+ treatment, however, plants decreased their N allocation to photosynthetic components, but increased their N allocation to the cell wall and elsewhere. Our findings demonstrated that within-leaf N allocation optimization is a key adaptive mechanism by which plants maximize their PNUE and biomass under predicted future global changes.

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“…Plant species can maintain metabolism and homeostasis via the modulation of fluidity and stability of plasma membrane under unfavorable situations [ 31 ], and saturated/unsaturated fatty acids play a particularly crucial role in membrane rigidity [ 32 ]. In this study, the levels of fatty acids increased in purple kohlrabi sprouts upon exposure to UVB radiation.…”

Section: Discussionmentioning

confidence: 99%

“…This finding implies that the increase in the levels of fatty acids may help sustain the metabolism and homeostasis in purple kohlrabi sprouts exposed to UVB radiation. Similarly, Zhou et al [ 32 ] have suggested that UVB radiation induces an increase in the levels of fatty acids, organic acids, and flavonoids in the leaves of Rhododendron chrysanthum irradiated by UVB radiation [ 31 ], and Moorthy and Kathiresan [ 33 ] have reported an increase in the levels of myristoleic acid in mangroves under high dose of UVB radiation [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolic Analyses and Evaluation of Antioxidant Activity in Purple Kohlrabi Sprouts after Exposed to UVB Radiation

et al. 2022

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Various metabolites act as plant defense molecules due to their antioxidant abilities. This study aimed to investigate the influence of UVB irradiation on the accumulation of metabolites, including primary metabolites (sugar, sugar alcohols, amino acids, organic acids, and an amine) and secondary metabolites (anthocyanins, fatty acids, and phenolic acids), and its synergistic antioxidant ability, in purple kohlrabi sprouts. Metabolite analyses revealed a total of 92 metabolites in the sprouts. Specifically, the levels of most amino acids increased after 24 h of UVB treatment, and then slightly decreased in the kohlrabi sprouts. The levels of most sugars and sugar alcohols increased after 24 h of UVB treatment and then decreased. The levels of TCA cycle intermediates and phenolic acids gradually increased during the UVB treatment. Furthermore, the levels of some fatty acids gradually increased during the UVB treatment, and the levels of the other fatty acids increased after 6 h of UVB treatment and then decreased. In particular, the levels of most anthocyanins, known to be strong antioxidants, gradually increased after 24 h of UVB treatment. In the in vitro ABTS scavenging assay, UVB-treated purple kohlrabi sprouts showed increased scavenging ability. This may be attributed to the increased accumulation of metabolites acting as antioxidants, in response to UVB treatment. This study confirmed that UVB irradiation induced the alteration of primary and secondary metabolism in the kohlrabi sprouts.

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Metabolic programming of Rhododendron chrysanthum leaves following exposure to UVB irradiation

Cited by 21 publications

References 46 publications

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

Improved Utilization of Nitrate Nitrogen Through Within-Leaf Nitrogen Allocation Trade-Offs in Leymus chinensis

Metabolic Analyses and Evaluation of Antioxidant Activity in Purple Kohlrabi Sprouts after Exposed to UVB Radiation

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