Metabolic profiling reveals growth stage variability in diatom exudates

Barofsky, Alexandra; Vidoudez, Charles; Pohnert, Georg

doi:10.4319/lom.2009.7.382

Cited by 94 publications

(81 citation statements)

References 38 publications

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“…In addition, structures and amount of other compounds released by diatoms have been found to change in accordance with the growth period [34]. Eventually, a complex chemical composition was generated in the water surrounding phytoplankton cells, which spread the information of algal cell nutrient content, resulting food quality, and edibility versus toxiticity to copepods [35].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence

Ismar

2018

Cell Physiol Biochem

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Background/Aims: As a model organism for a pleiomorphic marine planktonic primary producer, Phaeodactylum tricornutum has been studied on a molecular level under diverse cultural conditions. But little is known about its morphological, nutritional or transcriptomic responses under grazing stress. Methods: To assess microalgal molecular and cellular responses to grazer presence, we conducted transcriptome profiling in combination with growth rate, biovolume, fatty acid content, carbon and nitrogen content measurements in the model diatom Phaeodactylum tricornutum. RNA-sequencing was used to evaluate the transcriptomic response to grazing stress for P. tricornutum strain CCAP 1055/1. Results: Among the differentially expressed genes, we found down-regulation of genes involved in pathogen resistance, and in fatty acid biosynthesis pathways, while mitosis-involved genes were up-regulated. Experimentally testing morphological and biochemical responses in five strains of the species, we detected strain-specific significant effects of simulated grazing pressure in altered growth rates, biovolume and nutritional composition. Conclusion: Our research reveals the associated molecular and cellular responses to grazing effects in P. tricornutum and extends the understanding of co-evolutionary roles in regulating grazing defence between P. tricornutum and its grazer.

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Section: Introductionmentioning

confidence: 99%

Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence

Ismar

2018

Cell Physiol Biochem

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“…In this paper we present the results of metabolite analyses performed on the northern and southern strains of C. socialis from the same experiment described in Degerlund et al (2012) and relate these data to physiology data. A metabolic fingerprinting approach has already been shown to be able to separate phytoplankton species belonging to different genera (Chauton et al, 2003;Barofsky et al, 2009). Here, we demonstrate that metabolic fingerprinting can also be used to distinguish between closely related taxa, such as the 'forms' or pseudocryptic species of C. socialis.…”

Section: Introductionmentioning

confidence: 79%

“…The results from the phylogenetic analysis did not reveal any clear differences among the northern strains in the LSU rRNA gene (Degerlund et al, 2012). Barofsky et al (2009) reported differences in the exudates (metabolites excreted by the organisms) of the diatoms Skeletonema marinoi and Thalassiosira pseudonana during different growth phases, the stationary, exponential and declining phases of growth all being distinguishable. In our study, however, all strains at 13 C were in exponential growth phase, and yet there were differences in their metabolic fingerprints.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolomics is also increasingly applied, as a tool, in ecological and evolutionary studies (Macel et al, 2010). Only a few metabolomics studies have so far been performed on microalgae grown in different environmental conditions (Bo¨lling & Fiehn, 2005) or on the metabolite profiles of the different growth stages of microalgae (Barofsky et al, 2009(Barofsky et al, , 2010Vidoudez & Pohnert, 2012). Chemotaxonomic studies using metabolomic data on microalgae have been shown to be promising in species discrimination using NMR spectroscopy (Chauton et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic fingerprinting reveals differences between northern and southern strains of the cryptic diatomChaetoceros socialis

Huseby

Degerlund

Zingone

et al. 2012

European Journal of Phycology

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Morphology and molecular phylogeny constitute the structural elements of diatom taxonomy. These approaches do not, however, give information on the functioning of taxa. Additional methods to serve a more integrated and wide-ranging taxonomy have therefore been called for. Metabolic fingerprinting is one approach used within the field of metabolomics, often applied in classification of samples. Here we apply metabolic fingerprinting in a taxonomic study of a cryptic diatom species. Strains of the cosmopolitan diatom Chaetoceros socialis from two geographical areas; the north-east Atlantic and Arctic and the Gulf of Naples, were cultivated at three different temperatures; 2.5, 8 and 13 C. The strains from the two different geographical areas exhibited different growth rates as well as different photosynthetic efficiencies. Algal extracts, collected at the end of the growth experiments, were analysed by Ultra-Performance Liquid Chromatography High Resolution Mass Spectrometry. The two groups of strains were separated by principal component analysis of their metabolic fingerprints. Analysis of the data revealed both qualitative and quantitative differences in metabolite markers. These phenotypic differences reinforce differences also found for morphology, phylogenetic markers and growth rates, and point at different adaptive characteristics in organisms living under different temperature regimes.

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“…S. marinoi is known to produce polyunsaturated aldehydes (PUAs) mainly heptadienal, octadienal and octatrienal, which have previously been identified as the potential reasons for the detrimental effects on copepods (Ianora & Miralto 2010 and references therein). In addition, S. marinoi produces a wide variety of metabolites, the production of which changes dynamically during different growth phases (Vidoudez & Pohnert 2008, Barofsky et al 2009). During the study period, we would thus expect changes in the respiratory CR of the groups in relation to the abundance of PUAproducing S. marinoi.…”

Section: Introductionmentioning

confidence: 99%

Partition of planktonic respiratory carbon requirements during a phytoplankton spring bloom

Amin¹,

Båmstedt²,

Nejstgaard³

et al. 2012

Mar. Ecol. Prog. Ser.

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We studied the effect of variable phytoplankton biomass and dominance of the diatom Skeletonema marinoi on the planktonic community respiratory carbon requirement over a period of 14 d (14 to 28 April 2008) in 3 different mesocosms filled with natural water at Espegrend marine biological field station in Raunefjord, Norway. The carbon requirement was measured on mesozooplankton (the calanoid copepod Calanus finmarchicus) and 3 other size fractions of plankton -< 200 µm (dominated by microzooplankton), <15 µm (dominated by nanoplankton including most of the phytoplankton) and particles passing GF/C filters (dominated by bacterioplankton) -by measuring oxygen consumption using an optode system with 2 SensorDish Readers. The respiratory carbon requirement showed no clear trend over time for any of the 4 groups. The mesozooplankton contributed the least to the total community carbon requirement, corresponding to < 6% of primary production. In contrast, microzooplankton and nanoplankton consistently dominated the community carbon requirement, corresponding to > 50% of the primary production, while bacterioplankton showed an intermediate and variable contribution (ca. < 20% with a maximum of 50%). Feeding experiments on mesozooplankton (C. finmarchicus) 2 d before the peak in phytoplankton biomass showed that the copepods ingested from 2.4 to 4.3 times their respiratory carbon requirements, thus providing a high potential for growth. Respiratory carbon requirements of mesozooplankton were not significantly related to dominance or quantity of food available, whereas the respiratory carbon requirements of other groups were all related to the production of 22:6(n-3) fatty acid. The present study confirms the important role of microorganisms in the biological carbon transformation through the food web during a phytoplankton spring bloom. KEY WORDS: Plankton community · Respiratory carbon requirement · Energy transfer · Skeletonema marinoi · Mesozooplankton · Calanus Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 451: 15-29, 2012 16 Rivkin & Legendre 2001), whereas others suggest that bacterioplankton represent less (Ducklow et al. 2000) and that algal respiration (Lancelot et al. 1991) and microzooplankton (Calbet & Landry 2004) account for the bulk of community respiration in some eutrophic ecosystems. Consequently, the balance between photosynthesis and respiration will be strongly influenced by the quantitative relationships between microzooplankton, phytoplankton and bacterioplankton.Mesozooplankton respiration rate is an estimation of their minimum energetic requirements under given conditions, in terms of carbon (Hernández-León & Gómez 1996), thus representing the amount of energy necessary to maintain the structure and activity at this trophic level (Alcaraz & Packard 1989). Mesozooplankton is generally assumed to respire less than the smaller size groups, but, depending on the region studied and method of estimation, the mesozooplankton have been estim...

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Metabolic profiling reveals growth stage variability in diatom exudates

Cited by 94 publications

References 38 publications

Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence

Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence

Metabolic fingerprinting reveals differences between northern and southern strains of the cryptic diatomChaetoceros socialis

Partition of planktonic respiratory carbon requirements during a phytoplankton spring bloom

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