Metabolic Profiling of Urinary Organic Acids by Single and Multicolumn Capillary Gas Chromatography

Abstract

High-resolution gas chromatography (HRGC) and gas chromatography/mass spectrometry (GC/MS) are the techniques of choice to determine the retention indices of more than 200 organic acids as their trimethylsilyl (TMS) or oxime-trimethylsilyl derivatives. Several types of apolar and semipolar fused-silica capillary columns (OV-1, SE-52, and OV-1701), used to analyze and separate organic acids isolated from urine samples, are evaluated.

Gas chromatographic and mass spectrometric characterization of the organic acids extracted from some preparations containing lyophilized royal jelly

“…For instance, the LTPRI value 1265.2, which was calculated for TMS derivative of 4-hydroxypentanoic acid, coincides with the one, which was found experimentally in the work [21], i.e. 1265 (this value was not used in calculating the coefficients in corresponding equation for − 1 acids).…”

“…2). To construct graphs for , − 1 and 3-hydroxy acids we used LTPRI values from Table 2 and literature data [21] for homologues with less than eight carbon atoms.…”

Gas chromatographic–mass spectrometric investigation of volatile and extractable compounds of crude royal jelly

“…Registered mass spectra and LTPRI values were compared with literature data [30][31][32] and with our previously published data [29,33].…”

Gas chromatographic–mass spectrometric investigation of metabolites from the needles and roots of pine seedlings at early stages of pathogenic fungi Armillaria ostoyae attack

“…Therefore, to enhance the reliability of identification we used both mass spectra library search and linear temperature programmed retention indices of registered chromatographic peaks. The calculated LTPRI values of terpene compounds were compared with those presented by Adams (1995), and indices of TMS derivatives with those presented in papers (Gajewski et al 1982;Tuchman et al 1984;Lefevere et al 1989). Identification was considered reliable if the results of computer search at MS library were confirmed by the measured LTPRI values, the deviation of which from the literature data did not exceed ± 5 u.i.…”