Metabolic Profiling of Hoodia, Chamomile, Terminalia Species and Evaluation of Commercial Preparations Using Ultrahigh-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry

Avula, Bharathi; Wang, Yanhong; Isaac, Giorgis; Yuk, J; Wrona, Mark; Yu, Kate; Khan, Ikhlas A.

doi:10.1055/s-0043-109239

Cited by 13 publications

(7 citation statements)

References 34 publications

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“…Identified compounds from Terminalia catappa nut and Terminalia subspathulata fruit extracts using UHPLC-ESI-MS/MS Values based on total ion chromatogram (TIC) of Terminalia catappa for compounds common to both extracts detected only in the nuts of T. catappa but not in the fruits of T. subspathulata. It has been previously reported in the fruits of T. chebula(Avula et al, 2017).…”

mentioning

confidence: 72%

“…Oyeleye et al (2018) has previously reported the presence of gallic acid in the leaf and stembark of T. catappa. Compound 3, with [M-H]at m/z 331, was identified as glucogallin, a monogalloylglucose, based on tandem mass at m/z 211 and 169 for sequential loss of the glucose moiety, 162 amu (Avula et al, 2017;Kumar et al, 2015). Compounds 14 and 21, with [M-H]at m/z 483 and m/z 635, were identified as digalloylglucose and trigalloylglucose, respectively, based on the tandem mass arising from losses of the corresponding number of galloyl moieties (Singh et al, 2016).…”

Section: Gcms Metabolite Profile Of Hexane Fractionsmentioning

confidence: 99%

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Chemical Profiles of Terminalia catappa LINN Nut and Terminalia subspathulata KING Fruit

Yahaya¹,

Lee²,

Shaari³

2021

JTAS

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Terminalia catappa and Terminalia subspathulata are two species of the Combretaceae family of medium to large forest trees. The fruits of T. catappa are known for the edible nuts commonly known as tropical almonds due to their similarity in taste with almonds of commerce. Therefore, the chemical profiles of the fruits of the two Terminalia species were examined to ascertain their potential value for food or health uses. Gas chromatography-mass spectrometry (GCMS) and ultrahigh-pressure liquid chromatography-electrospray ionisation tandem mass spectrometry (UHPLC-ESI-MS/MS) techniques were employed to profile the extracts to ensure good coverage of the classes of metabolites of the fruit extracts. The GCMS results revealed that T. catappa nuts were rich in palmitic acid (33.2%), linoleoyl chloride (29.1%), and oxacyclohexadecan-2-one commonly known as pentadecanolide (16.2%). In comparison, the major constituents of T. subspathulata fruits were palmitic acid (18.1%) and its methyl ester, methyl palmitate (9.3%). Furthermore, a total of 38 compounds were putatively identified in the 70% aqueous methanolic extracts of both species via UHPLC-ESI-MS/MS analysis, comprising three organic acids, sixteen hydrolysable tannins, ten phenolic acids, eight flavonoids, and a diarylheptanoid. The GCMS- and liquid chromatography-mass spectrometry- (LCMS-) LCMS-based metabolite profiles obtained in the present study have revealed the diversity of chemical constituents in the T. catappa nuts and T. subspathulata fruits, potentially valorised as functional foods nutraceutical ingredients for plant-based health products.

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mentioning

confidence: 72%

Section: Gcms Metabolite Profile Of Hexane Fractionsmentioning

confidence: 99%

Chemical Profiles of Terminalia catappa LINN Nut and Terminalia subspathulata KING Fruit

Yahaya¹,

Lee²,

Shaari³

2021

JTAS

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“…As such, metabolomics provides a powerful tool for understanding the complete chemical makeup of an herbal product, which can be used for efficient and accurate quality control and authentication. Metabolomics characterizes the chemical relationships that underlie variations based upon genotype, origin (50), climate (51), or other biotic or abiotic interactions (52)(53)(54). While a variety of analytical inputs can be used to generate metabolome data -including Fourier-Transformed infrared spectroscopy (FT-IR), charged aerosol detection (CAD), ultraviolet-visible (UV/VIS) spectrophotometry, mass spectrometry (MS), and Nuclear Magnetic Resonance (NMR) spectroscopy -the two primary analytical approaches employed for the majority of metabolomics studies are liquid chromatography coupled to mass spectrometry (LC-MS) and NMR spectroscopy.…”

Section: Metabolomicsmentioning

confidence: 99%

Chemometric-Guided Approaches for Profiling and Authenticating Botanical Materials

Abraham

Kellogg

2021

Front. Nutr.

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Botanical supplements with broad traditional and medicinal uses represent an area of growing importance for American health management; 25% of U.S. adults use dietary supplements daily and collectively spent over $9. 5 billion in 2019 in herbal and botanical supplements alone. To understand how natural products benefit human health and determine potential safety concerns, careful in vitro, in vivo, and clinical studies are required. However, botanicals are innately complex systems, with complicated compositions that defy many standard analytical approaches and fluctuate based upon a plethora of factors, including genetics, growth conditions, and harvesting/processing procedures. Robust studies rely upon accurate identification of the plant material, and botanicals' increasing economic and health importance demand reproducible sourcing, as well as assessment of contamination or adulteration. These quality control needs for botanical products remain a significant problem plaguing researchers in academia as well as the supplement industry, thus posing a risk to consumers and possibly rendering clinical data irreproducible and/or irrelevant. Chemometric approaches that analyze the small molecule composition of materials provide a reliable and high-throughput avenue for botanical authentication. This review emphasizes the need for consistent material and provides insight into the roles of various modern chemometric analyses in evaluating and authenticating botanicals, focusing on advanced methodologies, including targeted and untargeted metabolite analysis, as well as the role of multivariate statistical modeling and machine learning in phytochemical characterization. Furthermore, we will discuss how chemometric approaches can be integrated with orthogonal techniques to provide a more robust approach to authentication, and provide directions for future research.

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“…0.5 mL of supernatant was used for UHPLC-MS analysis after a subsequent spin at 5000 g at 10 ºC for 20 minutes and transferring 250 µL of the sample into glass autosampler vials with inserts. For GC-MS (Gas Chromatography-Mass Spectrometry) analyses of primary polar metabolites, 0.5 mL water was added the remaining extract used above for the UHPLC preparation, sonicated for 5 min, extracted for 30min, and centrifuged at 3000 g. 0.5 mL of the polar extract was subsequently dried under nitrogen and derivatized using previously established protocols (35). Briefly, N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) with 1 % TMCS (2,2,2-Trifluoro-Nmethyl-N-(trimethylsilyl)-acetamide, Chlorotrimethylsilane) was used to derivatize the polar metabolites, after treatment with methoxyamine-HCl-pyridine.…”

Section: Ultra-high Performance Liquid Chromatography and Mass Spectrmentioning

confidence: 99%

Integrated metabolome and transcriptome analyses provide insight into colon cancer development by the gut microbiota

Busi

Lei

Sumner

et al. 2020

Preprint

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Colon cancer onset and progression is strongly associated with the presence, absence, or differences in relative abundances of certain microbial taxa in the gastrointestinal tract. However, specific mechanisms affecting disease susceptibility related to complex commensal bacterial mixtures are poorly understood. We used a multi-omics approach to determine how differences in the complex gut microbiome (GM) influence the metabolome and host transcriptome and ultimately affect susceptibility to adenoma development in a preclinical rat model of colon cancer. Fecal samples from rats harboring distinct complex GMs were analyzed using ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). We collected samples prior to observable disease onset and identified putative metabolite profiles that predicted future disease severity, independent of GM status. Transcriptome analyses performed after disease onset from normal epithelium and tumor tissues between the high and low tumor GMs suggests that the GM is correlated with altered host gene expression. Integrated pathway (IP) analyses of the metabolome and transcriptome based on putatively identified metabolic features indicate that bile acid biosynthesis was enriched in rats with high tumors (GM:F344) along with increased fatty acid metabolism and mucin biosynthesis. These data emphasize the utility of using untargeted metabolomics to reveal signatures of susceptibility and resistance and integrated analyses to reveal common pathways that are likely to be universal targets for intervention.Statement of significanceFecal metabolites, influenced by the gut microbiota, correlate with colon adenoma risk in a preclinical model of familial colon cancer.

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Metabolic Profiling of Hoodia, Chamomile, Terminalia Species and Evaluation of Commercial Preparations Using Ultrahigh-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry

Cited by 13 publications

References 34 publications

Chemical Profiles of Terminalia catappa LINN Nut and Terminalia subspathulata KING Fruit

Chemical Profiles of Terminalia catappa LINN Nut and Terminalia subspathulata KING Fruit

Chemometric-Guided Approaches for Profiling and Authenticating Botanical Materials

Integrated metabolome and transcriptome analyses provide insight into colon cancer development by the gut microbiota

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