Metabolic profiling of gender: Headspace-SPME/GC–MS and 1H NMR analysis of urine

Zhang, Shucha; Liu, Lingyan; Steffen, Debora M.; Ye, Tao; Raftery, Daniel

doi:10.1007/s11306-011-0315-2

Cited by 41 publications

(27 citation statements)

References 50 publications

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“…50 Our results are in agreement with previous observations indicating that females have lower plasma levels of BCAAs than males. 37, 51 A previous study also showed that a gender difference exists in the regulation of BCAA catabolism and suggested that female sex hormones are most important in producing the gender difference.…”

Section: Discussionsupporting

confidence: 93%

The Metabolite Profiles of the Obese Population Are Gender-Dependent

Xie

Zhao

et al. 2014

J. Proteome Res.

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Recent studies have identified that several amino acids, in particular, branched-chain amino acids (BCAAs), increased significantly in obese compared with lean individuals. Additionally, these metabolites were strongly associated with future diabetes, rendering them prognostic markers suitable for obese populations. Here we report a metabonomic study that reveals new findings on the role of these amino acid markers, particularly BCAAs, in a Chinese cohort including 106 healthy obese and 105 healthy lean participants. We found that the BCAAs were correlated with insulin resistance and differentially expressed in obese men, but not in obese women. The results were verified with two independent groups of participants (Chinese, n=105 and American, n=72), demonstrating that the serum metabolite profiles of the obese population are gender-dependent. The study supports the previous findings of a panel of several key metabolites as prognostic markers of obese population, and highlights the need to take into account gender differences when using these markers for risk assessment.

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Section: Discussionsupporting

confidence: 93%

The Metabolite Profiles of the Obese Population Are Gender-Dependent

Xie

Zhao

et al. 2014

J. Proteome Res.

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“…Cell debris was pelleted by centrifugation at 12,000 RPM for 2 min at 4°C. The supernatant was collected for metabolite profiling analysis using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) as described previously [27-29]. Metabolite levels were normalized to protein content.…”

Section: Methodsmentioning

confidence: 99%

1,25-Dihydroxyvitamin D regulation of glucose metabolism in Harvey-ras transformed MCF10A human breast epithelial cells

Zheng

Tayyari

Gowda

et al. 2013

The Journal of Steroid Biochemistry and Molecular Biology

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This study was designed to investigate the impact of 1,25 dihydroxyvitamin D (1,25(OH)2D) on glucose metabolism during early cancer progression. Untransformed and ras-oncogene transfected (ras) MCF10A human breast epithelial cells were employed to model early breast cancer progression. 1,25(OH)2D modified the response of the ras cells to glucose restriction, suggesting 1,25(OH)2D may reduce the ras cell glucose addiction noted in cancer cells. To understand the 1,25(OH)2D regulation of glucose metabolism, following four-day 1,25(OH)2D treatment, metabolite fluxes at the cell membrane were measured by a nanoprobe biosensor, [13C6]glucose flux by 13C-mass isotopomer distribution analysis of media metabolites, intracellular metabolite levels by NMR, and gene expression of related enzymes assessed. Treatment with 1,25(OH)2D reduced glycolysis as flux of glucose to 3-phosphoglycerate was reduced by 15% (P = 0.017) and 32% (P < 0.003) in MCF10A and ras cells respectively. In the ras cells, 1,25(OH)2D reduced lactate dehydrogenase activity by 15% (P < 0.05) with a concomitant 10% reduction in the flux of glucose to lactate (P = 0.006), and reduction in the level of intracellular lactate by 55% (P = 0.029). Treatment with 1,25(OH)2D reduced flux of glucose to acetyl-coA 24% (P = 0.002) and 41% (P < 0.001), and flux to oxaloacetate 34% (P = 0.003) and 33% (P = 0.027) in the MCF10A and ras cells, respectively, suggesting a reduction in tricarboxylic acid (TCA) cycle activity. The results suggest a novel mechanism involving the regulation of glucose metabolism by which 1,25(OH)2D may prevent breast cancer progression.

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“…Cell debris was pelleted by centrifugation at 12,000 RPM for 2 min at 48C. The supernatant was collected for metabolite profiling analysis using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) [21][22][23]. For metabolites analysis using NMR, water was removed by freeze-drying and the resulting residue reconstituted in 100 mM phosphate buffer (pH 7.4) prepared using deuterated water.…”

Section: Metabolomicsmentioning

confidence: 99%

Altered glucose metabolism in Harvey-rastransformed MCF10A cells

et al. 2013

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Metabolic reprogramming that alters the utilization of glucose including the “Warburg effect” is critical in the development of a tumorigenic phenotype. However, the effects of the Harvey-ras (H-ras) oncogene on cellular energy metabolism during mammary carcinogenesis are not known. The purpose of this study was to determine the effect of H-ras transformation on glucose metabolism using the untransformed MCF10A and H-ras oncogene transfected (MCF10A-ras) human breast epithelial cells, a model for early breast cancer progression. We measured the metabolite fluxes at the cell membrane by a selective micro-biosensor, [13C6]glucose flux by 13C-mass isotopomer distribution analysis of media metabolites, intracellular metabolite levels by NMR, and gene expression of glucose metabolism enzymes by quantitative PCR. Results from these studies indicated that MCF10A-ras cells exhibited enhanced glycolytic activity and lactate production, decreased glucose flux through the tricarboxylic acid (TCA) cycle, as well as an increase in the utilization of glucose in the pentose phosphate pathway (PPP). These results provide evidence for a role of H-ras oncogene in the metabolic reprogramming of MCF10A cells during early mammary carcinogenesis.

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Metabolic profiling of gender: Headspace-SPME/GC–MS and 1H NMR analysis of urine

Cited by 41 publications

References 50 publications

The Metabolite Profiles of the Obese Population Are Gender-Dependent

The Metabolite Profiles of the Obese Population Are Gender-Dependent

1,25-Dihydroxyvitamin D regulation of glucose metabolism in Harvey-ras transformed MCF10A human breast epithelial cells

Altered glucose metabolism in Harvey-rastransformed MCF10A cells

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