Metabolic profiling and investigations on crude extract of Olea europaea L. leaves as a potential therapeutic agent against skin cancer

Majumder, Durjoy; Debnath, Mousumi; Kumar, Krishna; Nath, Priyatosh; Debnath, Rahul; Sarkar, Chaitali; Prasad, Gbks; Verma, Yogesh Kumar; Maiti, Debasish

doi:10.1016/j.jff.2019.05.005

Cited by 10 publications

(12 citation statements)

References 29 publications

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“…The World Health Organization (WHO, Geneva, Switzerland) reports that globally, approximately 3 million skin cancers occur annually, of which less than 6% are melanomas [ 1 ]. Cutaneous malignant melanoma (CMM) developed by transformed melanocytes proliferating from the basal area of the epidermis, is the most aggressive form of diagnosed skin cancer [ 2 , 3 ]. Even though the incidence of melanoma is relatively low, its mortality is the highest among all skin cancers [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

Juszczak

Czarnomysy

Strawa

et al. 2021

IJMS

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Jasione montana L. (Campanulaceae) is used in traditional Belarusian herbal medicine for sleep disorders in children, but the chemical composition and biological activity have not been investigated. In this study, the activities of J. montana extracts, their fractions and main compounds were evaluated in amelanotic melanoma C32 (CRL-1585) cells and normal fibroblasts (PCS-201-012). The extracts and fractions were analyzed using liquid chromatography–photodiode array detection–electrospray ionization–mass spectrometry (LC–PDA–ESI–MS/TOF) to characterize 25 compounds. Further, three major and known constituents, luteolin (22) and its derivatives such as 7-O-glucoside (12) and 7-O-sambubioside (9) were isolated and identified. The cytotoxic activities against fibroblasts and the amelanotic melanoma cell line were determined using the fixable viability stain (FVS) assay. The influence of diethyl ether (Et2O) fraction (JM4) and 22 on apoptosis induction was investigated using an annexin V binding assay. The obtained results showed significant cytotoxicity of JM4 and 22 with IC50 values of 119.7 ± 3.2 and 95.1 ± 7.2 μg/mL, respectively. The proapoptotic potential after 22 treatment in the C32 human amelanotic melanoma cell line was comparable to that of vinblastine sulfate (VLB), detecting 29.2 ± 3.0% apoptotic cells. Moreover, 22 displayed less necrotic potential against melanoma cells than VLB. In addition, the influences of JM4 and 22 on the dysfunction of the mitochondrial membrane potential (MMP), cell cycle and activity of caspases 3, 8, 9, and 10 were established. The effects of JM4 on MMP change (74.5 ± 3.0% of the cells showed a reduced MMP) corresponded to the results obtained from the annexin V binding assay and activation of caspase-9. JM4 and 22 displayed a significant impact on caspase-9 (40.9 ± 2.4% of the cells contained active caspase-9 after JM4 treatment and 16.6 ± 0.8% after incubation with 22) and the intrinsic (mitochondrial) apoptotic pathway. Moreover, studies have shown that JM4 and 22 affect the activation of external apoptosis pathways by inducing the caspase-8 and caspase-10 cascades. Thus, activation of caspase-3 and DNA damage via external and internal apoptotic pathways were observed after treatment with JM4 and 22. The obtained results suggest that J. montana extracts could be developed as new topical preparations with potential anticancer properties due to their promising cytotoxic and proapoptotic potential.

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Section: Introductionmentioning

confidence: 99%

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

Juszczak

Czarnomysy

Strawa

et al. 2021

IJMS

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“…However, due to the wide range of plant matrices, it can be assumed that this effect may be caused by multicomponent synergism in this fraction. 25 In the B16F10 line, an apoptosis-stimulating effect was also shown by the hydromethanolic extract of Biophytum sensitivum, participating in the regulation of Bcl-2 and p53 genes and catalyzing the expression of the aforementioned caspase-3. In addition, Guruvayoorappan and co-authors reported that an extract rich in flavonoids is characterized by an antimetastatic nature and the inhibition of pro-inflammatory cytokines that play a significant role in chemoprevention, such as TNF-α, IL-1β, IL-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF).…”

Section: Role Of Luteolin and Its Derivative-rich Extracts In Skin Ca...mentioning

confidence: 98%

“…Medicinal plants and their bioactive compounds have been successfully used for years as complementary therapies. In addition, the research performed with multiple cancer cell lines and animal models including skin cancer proves that these combinations can suppress many stages of development and progression of cancer cells by influencing a number of mechanisms, such as the cell cycle, and by inhibiting angiogenesis and proliferation or activating proapoptotic and pro‐survival proteins 22–25 . Currently, various formulations in the market contain compounds of natural origin for use in skin cancer or precancerous conditions such as Birch Bark ointment , Curaderm® , or Cansema® 26 .…”

Section: Introductionmentioning

confidence: 99%

Skin cancer, including related pathways and therapy and the role of luteolin derivatives as potential therapeutics

Juszczak

Wölfle

Končić

2022

Medicinal Research Reviews

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Cutaneous malignant melanoma is the fastest growing and the most aggressive form of skin cancer that is diagnosed. However, its incidence is relatively scarce compared to the highest mortality rate of all skin cancers. The much more common skin cancers include nonmelanoma malignant skin cancers. Moreover, over the past several decades, the frequency of all skin cancers has increased much more dynamically than that of almost any other type of cancer. Among the available therapeutic options for skin cancers, chemotherapy used immediately after the surgical intervention has been an essential element. Unfortunately, the main problem with conventional chemopreventive regimens involves the lack of response to treatment and the associated side effects. Hence, there is a need for much more effective anticancer drugs. Correspondingly, the targeted alternatives have involved phytochemicals, which are safer chemotherapeutic agents and exhibit competitive anticancer activity with high therapeutic efficacy. Among polyphenolic compounds, some flavonoids and their derivatives, which are mostly found in medicinal plants, have been demonstrated to influence the modulation of signaling pathways at each stage of the carcinogenesis process, which is also important in the context of skin cancers. Hence, this review focuses on an exhaustive overview of the therapeutic effects of luteolin and its derivatives in the treatment and prevention of skin cancers. The bioavailability and structure–activity relationships of luteolin derivatives are also discussed. This review is the first such complete account of all of the scientific reports concerning this particular group of natural compounds that target a specific area of neoplastic diseases.

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“…In recent years, it has been found that plant extracts and natural bioactive compounds from plants show pharmacological activity against different cancers [36,37]. In the present study, HepG2 cells were selected to assess the anticancer cell proliferation activity of the leaf extracts (Table 4).…”

Section: Anti-hepg2 Cell Proliferationmentioning

confidence: 99%

Comprehensive profiling of phytochemical compounds, antioxidant activities, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential of Elaeagnus mollis leaf extracts

Kong

et al. 2020

PLoS ONE

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The aim of this work was to enrich the knowledge on the potential applications of Elaeagnus mollis leaf extracts. For this purpose, the bioactive compounds (phenolic, flavonoid, alkaloid, proanthocyanidin, chlorophyll and carotene content), antioxidant activity, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential (AChE and BChE) of E. mollis leaves which obtained from different habitats were quantitatively analyzed using various solvents (water, methanol, ethanol, and n-hexane). The results showed that the methanol extracts exhibited the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and the water extracts showed the best antioxidant activity in the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging activity, ferric reducing antioxidant power (FRAP), and reducing power (RP) assays. Moreover, the methanol extracts showed the best inhibitory activity against cholinesterase and HepG2 cancer cells. Correlation analysis revealed that the high antioxidant and anti-HepG2 cell proliferation activities were mainly attributed to the total phenolics, flavonoids, and proanthocyanidins while AChE inhibition was attributed to the total alkaloid and carotene content. The statistical results showed that the effect of habitats was lower than that of different solvents used. Additionally, the metabolic profiles of E. mollis leaves were evaluated using HPLC-ESI-Q TRAP-MS/MS, and a total of 1,017 chemical components were detected and classified into 23 classes. The organic acids and derivatives ranked the first, followed by flavone, amino acid and derivatives, and so on. In conclusion, the effects of different solvents were more significant than the effects of different habitats and the methanol extracts of E. mollis leaves could be used as an effective source of functional active components, provide benefits to physical health care and be applied to the food and pharmaceutical industries.

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Metabolic profiling and investigations on crude extract of Olea europaea L. leaves as a potential therapeutic agent against skin cancer

Cited by 10 publications

References 29 publications

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

Skin cancer, including related pathways and therapy and the role of luteolin derivatives as potential therapeutics

Comprehensive profiling of phytochemical compounds, antioxidant activities, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential of Elaeagnus mollis leaf extracts

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