Metabolic profiling and biological activities of the aerial parts of Micromeria imbricata Forssk. growing in Saudi Arabia

Al‐Yousef, Hanan M.; Khan, SI; El-Sayed, May A.; Amina, Musarat; Adel, Rasha; Hаssаn, Wаfаа S.; Abdelaziz, Sahar

doi:10.1016/j.sjbs.2021.05.077

Cited by 8 publications

(5 citation statements)

References 46 publications

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“…Yet, the ethanol extract of roots showed the top quantity as percentage of inhibition at low concentration (Figure 2). At the concentration of 100 µg/mL for the ethanol extracts, our results showing the same range of inhibition (48-65 %), are in agreement with the previously cited bibliography [23].…”

Section: Antioxidant Activity By Dpph Methodssupporting

confidence: 93%

GC–MS Based Metabolites Profiling, In Vitro Antioxidant, Antibacterial, and Anti-Cancer Properties of Different Solvent Extracts from Leaves, Stems, Roots, and Flowers of <em>Micromeria fruticosa</em> (Lamiaceae)

Abu‐Reidah¹,

Al-Nuri²,

Afifi³

et al. 2022

Preprint

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This study assesses the secondary metabolites, minerals, antimicrobial, antioxidant, and anticancer properties of Micromeria fruticosa plant different botanical parts (leaf, stem, root, flower) extracted with various solvents. The plant samples were sequentially obtained using different solvents (n-hexane, ethanol and water) through steeping. Then, each of the extracts was further analyzed by using gas chromatography–mass spectrometry (GC-MS). Moreover, the extracts were bio-assayed to test their antioxidant, antibacterial, and anti-cancer activities. Quali-quantitative analysis of M. fruticosa crude extracts revealed the occurrence of 27 secondary metabolites were identified including mainly monoterpenes, sesquterpenes, and fatty acids, with varying quantities. Some of the major bioactive compounds included, Menthone (5.42-30.05%), Oleamide (3.40-32.20%), Pulegone (10.66-64.1%), and Menthol (3.61-100.0%), which were detected mostly in all plant parts with significant quantities. Several antioxidant minerals, mainly, Fe, Zn, and Mn, were detected with the highest amounts in the Micromeria water extracts. Results from antimicrobial assays showed that the water extract of leaves exhibited the highest DPPH scavenging activity (89.73%) followed by the water extract of flowers (80.07%) at a concentration of 100 μg/mL. The water extract of stems showed greater antimicrobial activity against all the tested gram negative and positive bacteria (Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Escherichia coli, and Shigella sonnie). The leaves ethanolic and stem aqueous extracts had a strong antimicrobial activity against E. coli. and C. albicans. Flower aqueous extract demonstrated the highest cytostatic effect on the colon cell line by reducing viability up to 30.4%, followed by the leaf ethanol extract with 38.6% cell viability reduction at 1000 µg/mL. In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the different extracts could be correlated to the presence of additional bioactive compounds. Our results suggest that M. fruticosa plant is a promising source of natural products with antioxidant, anti-microbial and anti-cancer properties for potential nutraceutical, therapeutic, and functional food applications. , , , , , ,

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Section: Antioxidant Activity By Dpph Methodssupporting

confidence: 93%

GC–MS Based Metabolites Profiling, In Vitro Antioxidant, Antibacterial, and Anti-Cancer Properties of Different Solvent Extracts from Leaves, Stems, Roots, and Flowers of <em>Micromeria fruticosa</em> (Lamiaceae)

Abu‐Reidah¹,

Al-Nuri²,

Afifi³

et al. 2022

Preprint

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show abstract

“…Yet, the ethanol extract of roots showed the highest percentage of inhibition at a low concentration (Figure 2). At an ethanol extract concentration of100 µg/mL, our results showed the same range of inhibition (48-65%), which is in agreement with the previously cited bibliography [25]. In aqueous and hexane extracts, the leaf displayed functional inhibition at a low concentration of 2 µg/mL.…”

Section: Dpph Antioxidant Activitysupporting

confidence: 92%

GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae)

et al. 2022

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The present study assessed the metabolites and bioactivities of Micromeria fruticosa plant parts extracted with various solvents (ethanol, n-hexane, and water) through the steeping extraction method. Thereafter, the extracts were analyzed using GC-MS. Moreover, the extracts were tested for their antioxidant, antimicrobial, and antitumor activities. The quali-quantitative analysis of Micromeria fruticosa crude extracts revealed the occurrence of 27 secondary metabolites. Some major bioactives identified were menthone, oleamide, pulegone, and menthol. Numerous antioxidant minerals, viz., Fe, Zn, and Mn, were present. The water extract of leaves exhibited the highest DPPH scavenging activity (89.73%), followed by the water extract of flowers (80.07%) at 100 μg/mL. The stems’ water extract demonstrated greater antimicrobial activity against all the bacteria species tested. The ethanolic leaf and aqueous stem extracts exhibited strong activity against C. albicans and E. coli. Flowers’ aqueous extract demonstrated the highest cytostatic effect on the colon cell line by reducing viability, followed by the leaves’ ethanol extract. The extraction solvents influenced the recovery of phytocompounds, and the highest pharmacological activities of the different extracts could be correlated to the presence of additional bioactives. Our results suggest that the Micromeria fruticosa plant is a favorable source of natural products with promising properties for potential nutraceutical and functional food applications.

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“…DPPH • free radicals are widely used for investigating the preliminary radical scavenging effect of the plant extract [ 24 ]. Scavenging of DPPH • radical is associated with lipid peroxidation inhibition [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Unprecedented Insights on Chemical and Biological Significance of Euphorbia cactus Growing in Saudi Arabia

Al-Hamoud

Khan

Amina

et al. 2022

Plants

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Euphorbia cactus Ehrenb ex Boiss. is a plant species reported from central Africa and the southern Arabian Peninsula, belonging to the family of Euphorbiaceae. The plant has ethnobotanical values and is well-known for its milky latex, which has been turned into medicine to treat various ailments. To the best of our knowledge, there have been no literature reports available on phytochemical constituents and antiproliferative mechanism of E. cactus. In the current study, the phytochemical investigation of E. cactus methanolic extract (ECME) resulted in the isolation and characterization of four secondary metabolites, which are reported for the first time from this plant species. In addition, the results of 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and ferrous ion chelating (FIC) assays expressed maximum antioxidant activity by ECME and the isolated phytochemicals. Furthermore, ECME exerted a promising antiproliferative effect against different cancer cell lines, and the A549 lung cancer cells were the most sensitive with an IC50 value of 20 µg/mL. The antiproliferative action of ECME in A549 cells was associated with cell accumulation in the G2/M phase and an increase in early and late apoptosis. In addition, RT-PCR and western blot analysis revealed that ECME decreased the anti-apoptotic (Bcl-2) expression, while the expression of pro-apoptotic (Bax) and caspase-3 were increased. This study provides the first insight into the phytochemical constituents and the antiproliferative mechanism of ECME, implying that it could be exploited as a promising natural source for developing new cancer therapies. Further preclinical research is warranted to support the current results.

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Metabolic profiling and biological activities of the aerial parts of Micromeria imbricata Forssk. growing in Saudi Arabia

Cited by 8 publications

References 46 publications

GC–MS Based Metabolites Profiling, In Vitro Antioxidant, Antibacterial, and Anti-Cancer Properties of Different Solvent Extracts from Leaves, Stems, Roots, and Flowers of <em>Micromeria fruticosa</em> (Lamiaceae)

GC–MS Based Metabolites Profiling, In Vitro Antioxidant, Antibacterial, and Anti-Cancer Properties of Different Solvent Extracts from Leaves, Stems, Roots, and Flowers of <em>Micromeria fruticosa</em> (Lamiaceae)

GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae)

Unprecedented Insights on Chemical and Biological Significance of Euphorbia cactus Growing in Saudi Arabia

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