Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry

Abstract

Danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the traditional Chinese medicines (TCMs) most commonly used for the treatment of cardiovascular and cerebrovascular diseases. However, little is known about the chemical and metabolic profiles of danshen in vitro or in vivo. In particular, more information is needed in relation to the 50% ethanol extracts usually used in danshen formulations such as Fufang Xueshuantong Capsules and Fufang Danshen tablets. High-performance liquid chromatography coupled with a linear ion trap-Orbitrap mass spectrometer (HPLC-LTQ-Orbitrap) provides a sensitive and accurate method for analyzing the composition of samples. This method was used to determine the in vitro and in vivo chemical and metabolic profiles of danshen. Sixty-nine components of danshen extract and 118 components of danshen in rat plasma, urine, feces, and bile were unambiguously or tentatively identified. These results not only revealed the material composition of danshen, but also provided a comprehensive research approach for the identification of multi-constituents in TCMs.

Exploring the Traditional Uses of Thymbra capitata Infusion in Algarve (Portugal): Anti-Inflammatory, Wound Healing, and Anti-Aging

“…The fragmentation patterns of Peaks 4 and 5 were identical to the molecular ion [M − H] − at m / z 537 and the base peaks at m / z 339 in MS 2 and MS 3 due to the loss of a danshensu moiety [M − H − 198] − . Thus, Peaks 4 and 5 were tentatively assigned as salvianolic acid H/I [ 30 , 31 ].…”

Danshen-Shanzha formula for the treatment of atherosclerosis: ethnopharmacological relevance, preparation methods, chemical constituents, pharmacokinetic properties, and pharmacological effects

“…Focusing on constituents of the compatibility of each single-herb, some of the work have been conducted on the bioactive materials of DSF currently. Research has revealed that proanthocyanidin B2, salvianolic acid B, and tanshinone IIA are the active ingredients responsible for the anti-atherosclerotic effect in DSF ( Pang et al, 2018 ). By using the HPLC tandem DAD technique, 5 components of DS (salvianolic acid A, salvianolic acid B, lithospermic acid, rosmarinic acid and danshensu) and 5 components of SZ (isoquercetin, epicatechin, proanthocyanidin B2, hyperoside and 3-caffeoylquinic acid) were obtained and identified by a fingerprint method in water decoction of DSF ( Zhang et al, 2013b ).…”

A Novel Based-Network Strategy to Identify Phytochemicals from Radix Salviae Miltiorrhizae (Danshen) for Treating Alzheimer’s Disease

“…Together with our previous study and literature reports [54,55], active components absorbed in plasma were collected. Data about target proteins for those compounds were accessed from following resources: ETCM database (http://www.tcmip.cn/ETCM/ accessed on 20 September 2021, yes), STITCH database 5.0 (http://stitch.embl.de/ accessed on 20 September 2021, Score > 0.7), Swiss target prediction database (http:// swisstargetprediction.ch/ accessed on 21 September 2021, Probability > 0), SEA database (http://sea.bkslab.org/ accessed on 21 September 2021, HUMAN) or Targetnet database (http://targetnet.scbdd.com/ accessed on 21 September 2021, Probability > 0.5).…”