Metabolic Profile of Agropyron repens (L.) P. Beauv. Rhizome Herbal Tea by HPLC-PDA-ESI-MS/MS Analysis

Bortolami, Martina; Matteo, P. Di; Rocco, Daniele; Feroci, Marta; Petrucci, Rita

doi:10.3390/molecules27154962

Cited by 6 publications

(10 citation statements)

References 55 publications

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“…Before using the samples, water activity and absolute humidity were measured in duplicate by a Schaller Humimeter Rh2 (Schaller Messtechnik GmbH, St. Ruprecht an der Raab, Austria); the microbiological analysis for the total aerobic count, yeasts and molds, lactic acid bacteria and enterobacteriaceae were performed as previously described [36]. All measurements were performed in duplicate.…”

Section: Raw Materialsmentioning

confidence: 99%

“…Starting from the mass spectral analysis in positive ionization (ES+), carried out by direct infusion of the sample into the ESI source, a set of signals were well evidenced, corresponding to protonated ions [M+H] + and/or to sodium [M+Na] + and/or potassium [M+K] + adducts, that are typically evidenced in positive ionization [36,38,39,[49][50][51][52]. The ES+ mass spectral profile of BSG is shown in Fig.…”

Section: Bsg Untargeted Analysis By Direct Infusion Esi-ms/ms Experim...mentioning

confidence: 99%

“…The phenolic profile of the aqueous extracts of threshes was investigated by HPLC-PDA-ESI-MS in SIR mode with a method previously developed [37] isoxanthohumol (IsoX), xanthohumol (X). Furthermore, comparison with literature [36,66] was used for the identification of 3-feruloylquinic acid (3FQA) and 4feruloylquinic acid (4FQA) that were quantitated as ferulic acid equivalents; ellagic acid was searched by comparison with standard; lastly, tryptophan was identified and quantitated in the same chromatographic run, as previously described [36]. The aqueous extracts of malts and the corresponding wort were also analyzed for a comparison.…”

Section: Bsg Targeted Analysis By Hplc-pda-esi-ms In Sir Modementioning

confidence: 99%

“…Immediately a poor of peaks chromatogram catches the eye, suggesting that the matrix might contain a few polar molecules (water is the extraction solvent), or very low content of compounds (soft dilution 1:10), or molecules with no absorption in the UV-vis range (200-800 nm), or molecules not suitable for the ESI mass spectral analysis because of the absence of moieties able to be deprotonated or to be protonated (a very similar pattern is in fact observed in the ES-and ES+ full scan chromatograms, even less abundant). However, all the chromatograms are characterized by a peak at low retention time (t R = 5.25 min) that was identified with standard and quantitated as tryptophan [36], and by another peak at high t R (32.99 min), that was not identified and called unknown B in Fig. 5.…”

Section: Bsg Targeted Analysis By Hplc-pda-esi-ms In Sir Modementioning

confidence: 99%

“…Tryptophan and the phenolic profile were investigated by the Selected Ion Recording (SIR) mode, that is a highly selective and sensitive technique, and quantitation of each detected compound was carried by using the corresponding calibration curve, calculated with equal-weighted leastsquares linear regression analysis of the SIR peak area against the standard nominal concentration, as previously reported [36][37][38][39]. Results are resumed in Table 5 (Ref.…”

Section: Bsg Targeted Analysis By Hplc-pda-esi-ms In Sir Modementioning

confidence: 99%

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Phytochemical Characterization of Malt Spent Grain by Tandem Mass Spectrometry also Coupled with Liquid Chromatography: Bioactive Compounds from Brewery By-Products

Matteo¹,

Bortolami²,

Curulli³

et al. 2023

Front. Biosci. (Landmark Ed)

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Background: Brewer's spent grain (BSG) is one of the main by-products of beer industry, little used because of its high moisture making it difficult to transport and store. Mainly used as animal feed and for energy production, the agro-industrial waste have recently attracted attention as source of bioactive compounds, with potential applications in many sectors as food, nutraceutical, pharmaceutical, cosmetic, food packaging. The present work focuses on BSG as potential source of valuable small-size bioactive compounds. Methods: Laboratory-made BSG was obtained by using four base malts for mashing. After drying, BSG was eco-friendly extracted with water and the extracts analyzed by untargeted ElectroSpray Ionization (ESI)-Mass Spectrometry (MS)/Mass Spectrometry (MS) (ESI-MS/MS) infusion experiments and by targeted High Performance Liquid Chromatography-PhotoDiodeArray-ElectroSpray Ionization-Mass Spectrometry (HPLC-PDA-ESI-MS) in Selected Ion Recording (SIR) mode analysis, to investigate the metabolic profile, the phenolic profile, the individual phenolic content, and tryptophan content. Aqueous extracts of malts and wort samples were also analyzed for a comparison. Data were statistically analyzed by ANOVA test. An explorative analysis based on Principal Component Analysis (PCA) was also carried out on malts, wort and threshes, in order to study correlation among samples and between samples and variables. Results: The untargeted ESI-MS/MS infusion experiments provided the mass spectral fingerprint of BSG, evidencing amino acids (γ-aminobutyric acid, proline, valine, threonine, leucine/isoleucine, lysine, histidine, phenylalanine and arginine) and organic and inorganic acids (pyruvic, lactic, phosphoric, valerianic, malonic, 2-furoic, malic, citric and gluconic acids), besides sugars. γ-Aminobutyric acid and lactic acid resulted predominant among the others. The targeted HPLC-PDA-ESI-MS in SIR mode analysis provided the phenolic profile of the polar fraction of BSG, evidenced tryptophan as the main residual metabolite in BSG (62.33-75.35 µg/g dry BSG), and catechin (1.13-4.24 µg/g dry BSG) as the representative phenolic antioxidant of not pre-treated BSG samples. The chemometric analysis of the individual compounds content in BSG, malt and wort evidenced similarities and differences among the samples. Conclusions: As main goal, the phytochemical characterization of BSG from base malts highlighted BSG as a potential source of small biomolecules, as tryptophan and catechin, besides γ-aminobutyric acid and lactic acid, opening to new perspectives of application for BSG. Strategies for their recovery are a future challenge. Moreover, ESI-MS/MS analysis was confirmed as a powerful tool for fast characterization of complex matrix. Last, results obtained by chemometric elaboration of data demonstrated the possibility to monitor a small number of molecules to ensure the quality of a final product.

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Section: Raw Materialsmentioning

confidence: 99%

Section: Bsg Untargeted Analysis By Direct Infusion Esi-ms/ms Experim...mentioning

confidence: 99%

Section: Bsg Targeted Analysis By Hplc-pda-esi-ms In Sir Modementioning

confidence: 99%

Section: Bsg Targeted Analysis By Hplc-pda-esi-ms In Sir Modementioning

confidence: 99%

Section: Bsg Targeted Analysis By Hplc-pda-esi-ms In Sir Modementioning

confidence: 99%

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Phytochemical Characterization of Malt Spent Grain by Tandem Mass Spectrometry also Coupled with Liquid Chromatography: Bioactive Compounds from Brewery By-Products

Matteo¹,

Bortolami²,

Curulli³

et al. 2023

Front. Biosci. (Landmark Ed)

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Medicinal Plants of the Flora of Kazakhstan Used in the Treatment of Skin Diseases

Berganayeva¹,

Kudaibergenova²,

Litvinenko³

et al. 2023

Preprint

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According to the eminent scholar, philosopher, and physician Avicenna, "the doctor has three tools: the word, the plant, the knife." The plant kingdom is recognized as humanity's earliest and most ancient healing source, employed for the management and prevention of illnesses. Tracing back through history, the most ancient documented proof of plants' utilization in medicine dates back to a Sumerian clay slab discovered in Nagpur roughly 5000 years ago. This artifact included a compilation of twelve medicinal recipes that involved over 250 diverse plant species. Sumerian healers extracted powders and infusions from plant roots and stems, while also crediting healing properties to pears and figs. Additionally, they utilized dried and ground young shoots of willow and plum trees, pine and fir needles as a component in compresses and poultices. Often, powders from animal and mineral sources were blended with those extracted from dried and crushed plants. Notably, in addition to water, wine and beer served as solvents. Thus, at least 80 centuries ago, people utilized the most uncomplicated medicinal plant-based preparations for treatment [1]. The "Pen T'Sao," an ancient Chinese text on roots and herbs, authored by Emperor Shen Nung approximately 2500 BC, contains descriptions of 900 medicaments (comprising dried components of medicinal plants). Several of these substances are still in use today, including Rhei rhisoma, camphor, Theae folium, Podophyllum, great yellow gentian, ginseng, datura, cinnamon bark, and ephedra [2]. Regarding Kazakh folk medicine, an area that has yet to be fully explicated and substantiated, it can be stated that the traditional medicinal knowledge of the Kazakh people transcends the mere treatment of ailments and rests on robust theoretical underpinnings. Oteiboydak Tleukabyluly (1388-1478), an astute healer and prominent figure in Kazakh folk medicine during the 15th century, comprehensively explicated the secrets of the healing art in his medical and ethnographic work "Medical Narrative," which he composed between 1466 and 1473 at the behest of az-Zhanibek Khan, who held him in high esteem as a great healer. This medical encyclopedia delineates the functions of various organs of the human body and provides a catalogue of the primary diseases associated with them. Furthermore, it includes a meticulous description of the methods used in traditional medicine at present, such as setting bones, listening to the pulse, and incantations. Through practical experimentation and experimentation conducted in the steppe laboratory, the healer formulated a total of 1,108 different medicinal compounds, of which 858 were derived from medicinal plants, 318 were extracted from animal organs, and roughly 60 were sourced from metals. The moniker "Teacher without a teacher" was bestowed on Oteiboydak Tleukabylov, who discovered methods for treating 1,050 different diseases [3]. At present, the employment of phytotherapy has gained widespread acceptance on a global scale. According to the World Health Organization's (WHO) global review of national policies concerning traditional, complementary, and alternative medicine, as well as the regulation of herbal medicines, there is an evident growth in the European and Asian market for herbal medicines [4]. Kazakhstan boasts a natural flora of over 6,000 plant species [5]. The exact number of medicinal plant species present in Kazakhstan remains uncertain, as the list continues to expand annually. More than 150 plant species have been employed in both official and folk medicine for various ailments. This review focuses on a selection of medicinal plants growing within the territory of the Republic of Kazakhstan that have traditionally been used to alleviate skin diseases. In conducting this study (2010-2023), emphasis was placed on the plants' phytochemical composition, with a particular focus on the principal components responsible for their therapeutic effects against inflammatory skin conditions such as dermatitis, atopic dermatitis, and eczema.

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Electrochemical Determination of Tryptophan Based on Gly@CDs Clusters Modified Glassy Carbon Electrode

Bortolami,

Di Matteo,

Mastrorilli

et al. 2024

Chemosensors

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A simple sensor for the quantitation of tryptophan (Trp) has been developed using a glassy carbon electrode (GCE) modified with electro-synthesized carbon dots functionalized with glycine (Gly@CDs). The surface functionalization with an amino acid led to the formation of large clusters of nanostructures. To our knowledge, this is the first study in which a Gly@CDs clusters modified GCE is used for the analysis of Trp. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) are the techniques used to study Trp electrochemical behavior in an alkaline solution using such an electrode. A linear concentration range was found for Trp from 5 × 10−6 to 5 × 10−3 mol L−1 with a detection limit (LOD) of 5 × 10−6 mol L−1. The reproducibility and repeatability data were satisfactory in terms of RSD%. Moreover, the stability during the time of the modified electrode was considered, evidencing interesting results. The described sensor was used for the determination of Trp in herbal tea and a dietary supplement, and the results were compared with those obtained with HPLC-ESI-MS in the Selected Ion Recording (SIR) mode as an independent method. The electrochemical sensor presents significant advantages in terms of low cost, portability, ease of handling and not requiring skilled personnel.

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Metabolic Profile of Agropyron repens (L.) P. Beauv. Rhizome Herbal Tea by HPLC-PDA-ESI-MS/MS Analysis

Cited by 6 publications

References 55 publications

Phytochemical Characterization of Malt Spent Grain by Tandem Mass Spectrometry also Coupled with Liquid Chromatography: Bioactive Compounds from Brewery By-Products

Phytochemical Characterization of Malt Spent Grain by Tandem Mass Spectrometry also Coupled with Liquid Chromatography: Bioactive Compounds from Brewery By-Products

Medicinal Plants of the Flora of Kazakhstan Used in the Treatment of Skin Diseases

Electrochemical Determination of Tryptophan Based on Gly@CDs Clusters Modified Glassy Carbon Electrode

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