Metabolic Profile, Biotransformation, Docking Studies and Molecular Dynamics Simulations of Bioactive Compounds Secreted by CG3 Strain

Abstract

Actinobacteria isolated from untapped environments and exposed to extreme conditions such as saltpans are a promising source of novel bioactive compounds. These microorganisms can provide new molecules through either the biosynthetic pathway or the biotransformation of organic molecules. In the present study, we performed a chemical metabolic screening of secondary metabolites secreted by the new strain CG3, which was isolated from a saltpan located in the Sahara of Algeria, via high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC-ESI-HRMS). The results indicated that this strain produced five new polyene macrolactams, kenalactams A–E, along with two known compounds, mitomycin C and 6″-hydroxy-4,2′,3′,4″ tetramethoxy-p-terphenyl. Furthermore, the CG3 isolate could have excellent properties for converting the aglycone isoflavone glycitein to the compounds 6,7-dimethoxy-3-(4-methoxyphenyl)chromen-4-one (50) and 6,7-dimethoxy-3-phenylchromen-4-one (54), and the isoflavone genistein can be converted to 5,7-dimethoxy-3-(4-methoxyphenyl)chromen-4-one (52). Docking studies and molecular dynamics simulations indicated that these three isoflavones, generated via biotransformation, are potent inhibitors of the target protein aromatase (CYP19A1); consequently, they can be used to prevent breast cancer risk in postmenopausal women.

Phytochemical analysis, biological activities of methanolic extracts and an isolated flavonoid from Tunisian Limoniastrum monopetalum (L.) Boiss: an in vitro and in silico investigations

“…The data were identical to those of the known flavonoid maesopsin-6- O -glucoside (Fig. 1 ), isolated for the first times in 1997 by Li et al 30 from the root bark of Ceanothus americanus (family Rhamnaceae) 32 . Different protons of compound 1 , e.g., H-1”, showed two sets of equally intense signals in the NMR spectrum, due to the reversible nature of the hemiketal at C-2.…”

Characterization of Silver Carbonate Nanoparticles Biosynthesized Using Marine Actinobacteria and Exploring of Their Antimicrobial and Antibiofilm Activity

“…One of the well-known marine environments recognized for its geo-biodiversity is the Mediterranean coastline, which hosts more than 7% of the world's marine biodiversity, encompassing marine sediments, sponges, and algae, all of which are notable for their richness in actinomycete strains [12][13][14]. This coastal expanse holds the Algerian coast which spans 1622 km, and represents a yet untapped ecosystem, teeming a potential diversity of Actinobacteria strains, waiting to be discovered and harnessed [15][16][17]. Therefore, in response to the lack of published research material on such an important topic, this study sought to gather data on: (i) isolation, molecular identification, and assessment of the antimicrobial activity of marine actinomycetes isolated from sediments, algae, and marine sponges collected from the west coast of Algeria; (ii) the green synthesis of silver carbonate nanoparticles using marine Actinobacteria strain, followed by their characterization with a range of microscopic and spectroscopic techniques; and (iii) assessment of the biological activity of the biosynthesized silver carbonate nanoparticles.…”

Taxonomic Characterization, Antiviral Activity and Induction of Three New Kenalactams in Nocardiopsis sp. CG3

“…These compounds have been reported to display a potent biological activity [ 11 , 12 ] , including: antibacterial [ 15 , 18 ] , antifungal [ 19 ] , anticancer [ 20 ] , inflammatory [ 21 ] , antiparasite [ 22 ] . Therefore, species that belong to the Nocardiopsis genus, received a great attention , since they can provide a wide number of novel secondary metabolites which can help in creating new drugs in order to treat different serious disease [ 23 ] .…”