Metabolic Profile and Evaluation of Biological Activities of Extracts from the Stems of Cissus trifoliata

Abstract

Cissus trifoliata (L.) L belongs to the Vitaceae family and is an important medicinal plant used in Mexico for the management of infectious diseases and tumors. The present study aimed to evaluate the metabolic profile of the stems of C. trifoliata and to correlate the results with their antibacterial and cytotoxic activities. The hexane extract was analyzed using gas chromatography coupled with mass spectrometry (GC-MS) and the CHCl3-MeOH and aqueous extracts by ultraperformance liquid chromatography quadrupole time of fly mass spectrometry (UPLC-QTOF-MS). The antibacterial activity was determined by broth microdilution and the cytotoxicity was evaluated using MTS cell proliferation assay. Forty-six metabolites were putatively identified from the three extracts. Overall, terpenes, flavonoids and stilbenes characterize the metabolic profile. No antibacterial activity was found in any extract against the fifteen bacteria strains tested (MIC >500 µg/mL). However, high cytotoxic activity (IC50 ≤ 30 µg/mL) was found in the hexane and aqueous extracts against hepatocarcinoma and breast cancer cells (Hep3B, HepG2 and MCF7). This is the first report of the bioactive compounds of C. trifoliata stems and their antibacterial and cytotoxic properties. The metabolic profile rich in anticancer compounds correlate with the cytotoxic activity of the extracts from the stems of C. trifoliata. This study shows the antitumor effects of this plant used in the traditional medicine and justifies further research of its anticancer activity.

Metabolomic Profile and Cytotoxic Activity of Cissus incisa Leaves Extracts

“…Further investigations on CHCl 3 /MeOH extract led to isolation of several compounds such as: ceramides, cerebrosides, β-sitosterol, β-sitosterol-D-glucopyranoside, α-amyrin-3-O-β-D-glucopyranoside, and 2,3-dihydroxypropyl tetracosanoate [9,10]. Another study reported the chemical and biological profile of the stems of this plant [11]. In spite of the above, and to the best of our knowledge, there are no previous investigations about the cytotoxic activities related to the leaves of this plant.…”

Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of Jatropha cordata Bark Extracts

“…The fatty compounds´ abundance is directly related to their polarity and inversely related to their molecular weight ( Table 2 ). Overall, the compounds identified in the hexane extract are common constituents of the cuticles and membranes of most plants [ 20 ].…”

Bioassay-Guided Identification of the Antiproliferative Compounds of Cissus trifoliata and the Transcriptomic Effect of Resveratrol in Prostate Cancer Pc3 Cells

“…Hence, the compounds in the bioactive fraction might act in synergy and contribute to the antitumor properties associated with C. trifoliata in traditional medicine. According to other studies, the content of terpenes, flavonoids, and stilbenes plays a major role in the anticancer properties of plants from the genus Cissus [ 5 , 21 , 22 ]. For example, from the fractionation of the ethanolic extract of the aerial part of C. quadrangularis , a bioactive fraction against MCF7 breast cancer cells in vitro with a high content of the flavonoids quercetin and rutin was obtained [ 21 ].…”

“…The culture was incubated at 37 °C in a humidified atmosphere of 5% CO 2 . The fractions F1–F5 were composed mainly of alkanes, fatty acids, esters, and sterols, hence their evaluation was neglected since we previously reported the cytotoxicity of the lipidic content of C. trifoliata stems against PC3 cells [ 5 ]. The fractions F6-13 of the CHCl 3 -MeOH extract and the positive control Taxol were dissolved in DMSO (≤0.6%) in supplemented culture medium.…”

“…In our preliminary screening, hexane, chloroform, and aqueous extracts from the stems of C. trifoliata showed cytotoxic activity in vitro against cancer cells from the liver, breast, cervix, lung, and prostate. The metabolic profile of the stems of C. trifoliata was characterized by a high content of terpenes, sterols, flavonoids, and stilbenes with anticancer activities [ 5 ]. Additionally, our results were consistent with the traditional use of C. trifoliata in the Mexican and South American ethnomedical systems for the management of tumors [ 6 , 7 , 8 ].…”