Metabolic processes involved in repair of Escherichia coli cells damaged by exposure to acid mine water

Wortman, Alan T.; Bissonnette, Gary K.

doi:10.1128/aem.54.8.1901-1906.1988

Cited by 9 publications

(2 citation statements)

References 35 publications

(41 reference statements)

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“…Peptidoglycan synthesis Wortman and Bissonnette (1988) of ultraviolet (UV)-LED illumination. Choi, Park, Choi, Kim, and Chun (2015) found that when Listeria monocytogenes in sterile distilled water, treated with UV light (12.4 mJ/cm 2 ), there was no sublethal damage cells occurred.…”

Section: Discussionmentioning

confidence: 99%

Effects of 405 nm light‐emitting diode treatment on microbial community on fresh‐cut pakchoi and antimicrobial action against Pseudomonas reinekei and Pseudomonas palleroniana

Zhang

Xie

2021

Journal of Food Safety

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Pakchoi (Brassica rapa L. Chinensis) is an important cruciferous vegetable crop in East and Southeast Asia. This research aimed to investigate the change of microbial community and antibacterial effect of 405 nm light-emitting diode (LED 405 nm ) illumination (15 μ mol/(m 2 Á s)) against spoilage bacteria on the surface of agar media and fresh-cut pakchoi. High-throughput community sequencing was used to quantify bacterial abundance and 16S rRNA was used to analyze the bacterial community composition on fresh-cut pakchoi treated with or without LED 405 nm storage at 4 C. And alkaline phosphatase (AKP) activity and metabolic inhibition assays were selected to measure the mechanism of bacterial inhibition. The results showed that Pseudomonas spp. were able to multiply and cause spoilage of fresh-cut pakchoi at 4 C. Nevertheless, the majority of Pseudomonas spp. isolated from spoilage pakchoi were Pseudomonas reinekei and Pseudomonas palleroniana which were considered as representative specific spoilage microorganisms in pakchoi. Furthermore, 405 nm light treatment retarded sensory deterioration, inhibited microbial growth. AKP activity and metabolic inhibition assays confirmed the inhibitory effect of light treatment. In brief, 405 nm LED illumination has potential as a preservative to maintain quality, extend the shelf life for 6 days of fresh-cut pakchoi and abled to control P. reinekei and P. palleroniana growth on fresh-cut pakchoi at 4 C. | INTRODUCTIONFresh-cut vegetables refer to vegetables processed by washing, sorting, peeling, and slicing (Pan & Sasanatayart, 2016). However, during the processing of fresh-cut vegetables, because the phenols are oxidized by polyphenol oxidase reaction, it is easy to turn brown, red, and black (Chisari, Barbagallo, & Spagna, 2008). Different from enzymatic browning of vegetables, Pseudomonas spp. mainly causes tissue rot of fresh-cut vegetables, and has been proved to result in rapid browning reaction on cut surface of pakchoi (Wu et al., 2019).Research studies revealed that Pseudomonas spp. was correlated with the deterioration of lettuce (Federico et al., 2015), whereas Pseudomonas cichorii caused quality decline of head lettuce such as dark-brown and necrotic spots on leaves (Grogan, 1977). Moreover, pectolytic enzymes produced in Pseudomonas viridiflava and Pseudomonas chlororaphis caused organoleptic properties' decline and spoilage in fresh-cut vegetables (Lee et al., 2013;Liao, 1988).Studies have revealed that 405 nm visible light is effective to eliminating various bacterial strains (

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Section: Discussionmentioning

confidence: 99%

Effects of 405 nm light‐emitting diode treatment on microbial community on fresh‐cut pakchoi and antimicrobial action against Pseudomonas reinekei and Pseudomonas palleroniana

Zhang

Xie

2021

Journal of Food Safety

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“…Depending on the extent of membrane damage, larger solutes such as ATP or DNA (500–700 Da) are also released from the cell (Zhen et al, 2013 ; Singh et al, 2016 ). Although bacteria possess membrane repair mechanisms, the duration and the extent of membrane damage (i.e., pore size) may lead to bacterial cell death (Wortman and Bissonnette, 1988 ; Vigouroux et al, 2020 ). As the model developed in this work predicts the timely repair of pores, it could be integrated with future work to estimate the cumulative probability of survival and life expectancy.…”

Section: Discussionmentioning

confidence: 99%

A Diffusion Model to Quantify Membrane Repair Process in Listeria monocytogenes Exposed to High Pressure Processing Based on Fluorescence Microscopy Data

et al. 2021

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The effects of environmental stresses on microorganisms have been well-studied, and cellular responses to stresses such as heat, cold, acids, and salts have been extensively discussed. Although high pressure processing (HPP) is becoming more popular as a preservation method in the food industry, the characteristics of the cellular damage caused by high pressure are unclear, and the microbial response to this stress has not yet been well-explored. We exposed the pathogen Listeria monocytogenes to HPP (400 MPa, 8 min, 8°C) and found that the high pressure created plasma membrane pores. Using a common staining technique involving propidium iodide (PI) combined with high-frequency fluorescence microscopy, we monitored the rate of diffusion of PI molecules into hundreds of bacterial cells through these pores on days 0, 1, 2, 3, and 4 after pressurization. We also developed a mathematical dynamic model based on mass transfer and passive diffusion laws, calibrated using our microscopy experiments, to evaluate the response of bacteria to HPP. We found that the rate of diffusion of PI into the cells decreased over the 4 consecutive days after exposure to HPP, indicating repair of the pressure-created membrane pores. The model suggested a temporal change in the size of pores until closure. To the best of our knowledge, this is the first time that pressure-created membrane pores have been quantitatively described and shown to diminish with time. In addition, we found that the membrane repair rate in response to HPP was linear, and growth was temporarily arrested at the population level during the repair period. These results support the existence of a progressive repair process in some of the cells that take up PI, which can therefore be considered as being sub-lethally injured rather than dead. Hence, we showed that a subgroup of bacteria survived HPP and actively repaired their membrane pores.

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Comparison of measurement methods at determining the target sites injured by antimicrobials in Escherichia coli O157:H7 using metabolic inhibitors

Hyun

Lee

2021

Food Sci Biotechnol

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Metabolic processes involved in repair of Escherichia coli cells damaged by exposure to acid mine water

Cited by 9 publications

References 35 publications

Effects of 405 nm light‐emitting diode treatment on microbial community on fresh‐cut pakchoi and antimicrobial action against Pseudomonas reinekei and Pseudomonas palleroniana

Effects of 405 nm light‐emitting diode treatment on microbial community on fresh‐cut pakchoi and antimicrobial action against Pseudomonas reinekei and Pseudomonas palleroniana

A Diffusion Model to Quantify Membrane Repair Process in Listeria monocytogenes Exposed to High Pressure Processing Based on Fluorescence Microscopy Data

Comparison of measurement methods at determining the target sites injured by antimicrobials in Escherichia coli O157:H7 using metabolic inhibitors

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