Metabolic pools and the synthesis of macromolecules

Cowie, Dean B.; McClure, F. T.

doi:10.1016/0006-3002(59)90460-3

Cited by 126 publications

(37 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Uptake of 5-FC-2-,JC in C. albicans, distribution of radioactivity in the expandable pool, internal pool (as defined by C ow ie and M cC lure [1]; Kempner [7]), and RNA (see 'Materials and Methods'), as well as incor poration of the drug into rRNA and tRNA, were investigated as influenced by Ade, Urd or Cyt, which had exhibited the strongest -and, as far as Ade is concerned, most surprising -antagonism against 5-FC in the reversal experiments reported before [14], The uptake and distribution of radiolabelled 5-FC were first checked in the stationary growth phase of the yeast, i.e., at the same time as the fungistatic and antagonistic effects described in our previous paper [ 14]. By uptake, we mean the total radio active compond(s) found in the yeast cells after the particular period of incubation.…”

Section: Discussionmentioning

confidence: 99%

“…Further samples of the C. albicans cells were consecutively processed for the extraction of (1) the expandable pool, (2) the internal pool [1,7], and (3) the 'RNA fraction' = cold TCA insoluble fraction (see below): They were (1) extracted with water and (2) with cold 5% TCA. Both liquids were evaporated in the vacuum oven and the residue was redissolved in 2.0 ml distilled water, 0.5-ml aliquots of which were pipetted on ash-free filter tablets (Schleicher & Schiill).…”

Section: Uptake and Distribution Of S-fc-2-4cmentioning

confidence: 99%

See 1 more Smart Citation

Fungistatic Activity, Uptake and Incorporation of 5-FIuorocytosine in <i>Candida albicans, </i>as influenced by Pyrimidines and Purines

Polak

Scholer

1973

Pathobiology

View full text Add to dashboard Cite

Uptake of the radiolabelled antimycotic 5-fluorocytosine (5-FC) by Candida albicans and distribution of radioactivity in the cell fractions, expandable pool, internal pool, and RNA, as influenced by the three strong antagonists against the fungistatic activity of the drug, adenine, uridine and cytosine were studied. In the stationary growth phase of the yeast, a reciprocal correlation was found between the concentrations of the antagonists and the presence of radioactive compound(s) in the cells, particularly in RNA. The correlation was similar with radio-labelled cytosine which was studied comparatively, but, considering the proportions, the normal pyrimidine was preferred to its fluorinated analogue. In the logarithmic phase of yeast growth, two phases of transport were distinguished: a first phase of 5-FC uptake, when there was a transient accumulation of radioactivity in the internal pool, and a second one of incorporation into RNA. The addition of cytosine resulted in a marked impairment of 5-FC uptake in the expandable pool, preventing any significant passage to the internal pool and RNA, whereas with uridine only the incorporation into RNA was inhibited. With adenine, the picture was similar to that observed with cytosine, suggesting a hitherto unknown interference of purines with pyrimidine uptake in yeasts. Confirming the findings of Giégé and Weil [4] in Saccharomyces cerevisiae, it was proved that in C. albicans, cultured in the presence of 5-FC, 5-fluorouracil becomes incorporated in place of uracil in both rRNA and tRNA, without any 5-FC being detectable.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Uptake and Distribution Of S-fc-2-4cmentioning

confidence: 99%

Fungistatic Activity, Uptake and Incorporation of 5-FIuorocytosine in <i>Candida albicans, </i>as influenced by Pyrimidines and Purines

Polak

Scholer

1973

Pathobiology

View full text Add to dashboard Cite

show abstract

“…Nous en sommes ainsi venus à nous demander si les vacuoles emmagasinent des acides aminés. L'existence de «pools» de stockage et de «pools» métaboliques d'acides aminés a été postulée plusieurs fois par l'analyse de données cinétiques à l'aide de marquages radioactifs (Cowie et McClure, 1959).…”

Section: * **unclassified

Mise en évidence du rôle des vacuoles par l'étude du métabolisme de l'arginine chez la levure

Wiemken¹

1982

Bulletin de la Société Botanique de France. Actualités Botaniqu

View full text Add to dashboard Cite

Résumé.-Pratiquement la totalité des vacuoles des levures peuvent être isolées et purifiées. Entre autres h'Ubstances, ces vacuoles renferment de grandes quantités d'arginine liée à des polyphosphates. Comment la cellule peut-elle disposer de ce pool de réserve lorsque les conditions de l'environnement, notamment la disponibilité en azote, se modifient ?Summary.-Almost the totality of the vacuoles of yeast ceUs can be isolated and highly purified. Among many other substances the vacuoles contain large amounts of arginine bound to polyphosphates. How can the cells mobilize the storage pool of arginine in an environment changing with respect to the nitrogen source ?

show abstract

“…Various kinetic studies have well-documented the concept of metabolic pools within an organism, although their location within the cell is by no means positively established (Zalokar, 1961 ;Steward & Bidwell, 1962;Oaks, 1965). Two amino acid pools are generally recognized, an 'expandable' pool into which exogenous amino acids are accumulated and an 'internal' pool closely associated with protein synthesis (Cowie & McClure, 1959). Dawson (1965), experimenting with Candidu utilis, has shown that the second pool was not related to the amount of protein synthesis within the cell and has envisaged a pool of 'activated' amino acids.…”

Section: Integration Into Cellular Metabolismmentioning

confidence: 99%

Amino Acid Selection in Protein Biosynthesis

Peterson¹

1967

Biological Reviews

View full text Add to dashboard Cite

Metabolic pools and the synthesis of macromolecules

Cited by 126 publications

References 3 publications

Fungistatic Activity, Uptake and Incorporation of 5-FIuorocytosine in <i>Candida albicans, </i>as influenced by Pyrimidines and Purines

Fungistatic Activity, Uptake and Incorporation of 5-FIuorocytosine in <i>Candida albicans, </i>as influenced by Pyrimidines and Purines

Mise en évidence du rôle des vacuoles par l'étude du métabolisme de l'arginine chez la levure

Amino Acid Selection in Protein Biosynthesis

Contact Info

Product

Resources

About