Metabolic plasticity of the starchless mutant of Chlorella sorokiniana and mechanisms underlying its enhanced lipid production revealed by comparative metabolomics analysis

Wu, Mingcan; Hu, Zhang; Sun, Wenchao; Li, Yanhua; Zhou, Hantao; Han, Danxiang

doi:10.1016/j.algal.2019.101587

Cited by 47 publications

(41 citation statements)

References 39 publications

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“…However, if optimization of the culture conditions fails to work, we must continue to screen for suitable resistant strains or apply some other method, such as chemical mutagenesis (Wu et al. ) or gene manipulation (Simkovsky et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…In this respect, we can try to optimize the culture conditions to improve the lipid content of the resistant strain, such as through nitrogen depletion. However, if optimization of the culture conditions fails to work, we must continue to screen for suitable resistant strains or apply some other method, such as chemical mutagenesis (Wu et al 2019) or gene manipulation (Simkovsky et al 2012).…”

Section: How Does Poterioochromonas Malhamensis Select Chlorella?mentioning

confidence: 99%

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Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Wei

Wang

et al. 2019

J Eukaryotic Microbiology

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Grazing behaviour between protozoa and phytoplankton exists widely in planktonic ecosystems. Poterioochromonas malhamensis is a well‐known and widespread mixotrophic flagellate, which is recognized to play an important role within marine and freshwater planktonic ecosystems and regarded as the greatest contamination threat for mass algal cultures of Chlorella. In this study, a comprehensive range of factors, including morphological characters, biochemical compositions, and specific growth rate of ten species or strains of Chlorella, were evaluated for their effect on the feeding ability of P. malhamensis, which was assessed by two parameters: the clearance rate of P. malhamensis on Chlorella spp. and the specific growth rate of P. malhamensis. The results showed that the clearance rate of P. malhamensis was negatively correlated with cell wall thickness and specific growth rate of Chlorella spp., while the specific growth rate of P. malhamensis was positively correlated with carbohydrate percentage and C/N ratio and negatively correlated with protein, lipid percentage, and nitrogen mass. In conclusion, the factors influencing feeding selectivity include not only the morphological character and chemical composition of Chlorella, but also its population dynamics. Our study provides useful insights into the key factors that affect the feeding selectivity of P. malhamensis and provides basic and constructive data to help in screening for grazing‐resistant microalgae.

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Section: Discussionmentioning

confidence: 99%

Section: How Does Poterioochromonas Malhamensis Select Chlorella?mentioning

confidence: 99%

Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Wei

Wang

et al. 2019

J Eukaryotic Microbiology

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“…The cellular contents of the total fatty acids were quantified according to the methods described previously ( Wu et al, 2019 ). To measure the TAG contents, total lipids were extracted from 10 mg of the lyophilized algal biomass.…”

Section: Methodsmentioning

confidence: 99%

“…TAG was separated via thin layer chromatography (TLC). The TAG spots were scraped from the TLC silico gel glass plate and then trans-esterified to the fatty acid methyl esters for gas chromatography mass spectrometry analysis ( Wu et al, 2019 ). Ten milligrams of lyophilized algal biomass of each cell lines were used to determine the total carbohydrate content by using the phenol-sulfuric acid method described previously ( Dubois et al, 1956 ; Jia et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Identification and Biotechnical Potential of a Gcn5-Related N-Acetyltransferase Gene in Enhancing Microalgal Biomass and Starch Production

Cao

Zhao

et al. 2020

Front. Plant Sci.

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Microalgae are promising feedstocks for starch production, which are precursors for bioenergy and chemicals manufacturing. Though starch biosynthesis has been intensively studied in the green alga Chlamydomonas reinhardtii , regulatory mechanisms governing starch metabolism in this model species have remained largely unknown to date. We proposed that altering triacylglycerol (TAG) biosynthesis may trigger intrinsic regulatory pathways governing starch metabolism. In accordance with the hypothesis, it was observed in this study that overexpression of the plastidial lysophosphatidic acid acyltransferase gene (i.e. LPAAT1 ) in C. reinhardtii significantly enhanced TAG biosynthesis under nitrogen (N)-replete conditions, whereas the starch biosynthesis was enhanced in turn under N depletion. By the exploitation of transcriptomics analysis, a putative regulatory gene coding Gcn5-related N-acetyltransferase ( GNAT19 ) was identified, which was up-regulated by 11–12 times in the CrLPAAT1 OE lines. Overexpression of the cloned full-length CrGNAT19 cDNA led to significant increase in the starch content of C. reinhardtii cells grown under both N-replete and N-depleted conditions, which was up to 4 times and 26.7% higher than that of the empty vector control, respectively. Moreover, the biomass yield of the CrGNAT19 OE lines reached 1.5 g L -1 after 2 days under N-depleted conditions, 72% higher than that of the empty vector control (0.87 g L -1 ). Overall, the yield of starch increased by 118.5% in CrGNAT19 OE lines compared to that of the control. This study revealed the great biotechnical potentials of an unprecedented GNAT19 gene in enhancing microalgal starch and biomass production.

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“…To monitor cellular growth, cell numbers were counted by using a hemocytometer (Improved Neubauer, USA). Cell dry weight was measured according to the method described by Lee et al (2013) and Wu et al (Wu et al 2020;Wu et al 2019) with the following adjustments.5 -10 mL of microalgae suspension was filtered through a preheated (105°C, 24 h), pre-weighed glass microfiber filter (Whatman GF/C, 47 mm, UK). The filters were washed twice each with 20 mL of 0.5 M ammonium bicarbonate.…”

Section: Growth Measurementmentioning

confidence: 99%

Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6

Zhu

et al. 2020

Ann Microbiol

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Purpose: Under different abiotic-stress conditions, the unicellular green microalga Dunaliella salina accumulates large amounts of carotenoids which are accompanied by fatty acid biosynthesis. Carotenoids and fatty acids both possess long carbon backbones; however, the relationship between carotenoid and fatty acid metabolism is controversial and remains poorly understood in microalgae. Methods: In this study, we investigated the growth curves and the β-carotene, lutein, lipid, and fatty acid contents of D. salina Y6 grown under different abiotic-stress conditions, including high light, nitrogen depletion, and high salinity. Results: Both high-salinity and nitrogen-depleted conditions significantly inhibited cell growth. Nitrogen depletion significantly induced β-carotene accumulation, whereas lutein production was promoted by high light. The accumulation of lipids did not directly positive correlate with β-carotene and lutein accumulation under the three tested abiotic-stress conditions, and levels of only a few fatty acids were increased under specific conditions. Conclusion: Our data indicate that cellular β-carotene accumulation in D. salina Y6 positive correlates with accumulation of specific fatty acids (C16:0, C18:3n3, C14:0, and C15:0) rather than with total fatty acid content under different abiotic stress conditions.

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Metabolic plasticity of the starchless mutant of Chlorella sorokiniana and mechanisms underlying its enhanced lipid production revealed by comparative metabolomics analysis

Cited by 47 publications

References 39 publications

Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Identification and Biotechnical Potential of a Gcn5-Related N-Acetyltransferase Gene in Enhancing Microalgal Biomass and Starch Production

Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6

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