Metabolic pathways regulated by abscisic acid, salicylic acid and γ‐aminobutyric acid in association with improved drought tolerance in creeping bentgrass (<i>Agrostis stolonifera</i>)

Zhou, Li; Yu, Jingjin; Peng, Yan; Huang, Bingru

doi:10.1111/ppl.12483

Cited by 160 publications

(111 citation statements)

References 78 publications

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“…Further studies are required to establish a role for phloem B-type cells in long-distance signaling in plants (Shelp, 2012). In a study concerning drought stress tolerance with a Poaceae species, Agrostis stolonifera , GABA was shown to enhance the accumulation of GABA and of other amino acids (glycine, valine, proline, 5-oxoproline, serine, threonine, aspartic acid and glutamic acid) (Li et al, 2017). Interestingly, in barley plants it was shown that external application of GABA was able to induce action potentials (APs) accompanied by cytoplasmatic acidification short of the position of the stimulus (Felle and Zimmermann, 2007).…”

Section: Resultsmentioning

confidence: 99%

Evidence for GABA-Induced Systemic GABA Accumulation in Arabidopsis upon Wounding

et al. 2017

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The non-proteinogenic amino acid γ-aminobutyric acid (GABA) is present in all plant species analyzed so far. Its synthesis is stimulated by either acidic conditions occurring after tissue disruption or higher cytosolic calcium level. In mammals, GABA acts as inhibitory neurotransmitter but its function in plants is still not well understood. Besides its involvement in abiotic stress resistance, GABA has a role in the jasmonate-independent defense against invertebrate pests. While the biochemical basis for GABA accumulation in wounded leaves is obvious, the underlying mechanisms for wounding-induced GABA accumulation in systemic leaves remained unclear. Here, the Arabidopsis thaliana knock-out mutant lines pop2-5, unable to degrade GABA, and tpc1-2, lacking a wounding-induced systemic cytosolic calcium elevation, were employed for a comprehensive investigation of systemic GABA accumulation. A wounding-induced systemic GABA accumulation was detected in tpc1-2 plants demonstrating that an increased calcium level was not involved. Similarly, after both mechanical wounding and Spodoptera littoralis feeding, GABA accumulation in pop2-5 plants was significantly higher in local and systemic leaves, compared to wild-type plants. Consequently, larvae feeding on these GABA-enriched mutant plants grew significantly less. Upon exogenous application of a D2-labeled GABA to wounded leaves of pop2-5 plants, its uptake but no translocation to unwounded leaves was detected. In contrast, an accumulation of endogenous GABA was observed in vascular connected systemic leaves. These results suggest that the systemic accumulation of GABA upon wounding does not depend on the translocation of GABA or on an increase in cytosolic calcium.

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Section: Resultsmentioning

confidence: 99%

Evidence for GABA-Induced Systemic GABA Accumulation in Arabidopsis upon Wounding

et al. 2017

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“…ASM treatment resulted in significantly increased accumulations of the amino acids aspartic acid, glutamic acid, glutamine, glycine, isoleucine, lysine, serine, and threonine during heat stress compared to untreated plants. Increased accumulations of the amino acids aspartic acid, glutamic acid, glycine, and serine have previously been associated with increased levels of stress tolerance in turfgrasses (Jespersen et al, 2015; Li et al, 2016). The higher accumulation of these amino acids in ASM treated plants may be part of major shifts in metabolism and activation of defense pathways.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass

Jespersen

Huang

2017

Front. Plant Sci.

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Acibenzolar-S-methyl (ASM) is a synthetic functional analog of salicylic acid which can induce systemic acquired resistance in plants, but its effects on abiotic stress tolerance is not well known. The objectives of this study were to examine effects of acibenzolar-S-methyl on heat or drought tolerance in creeping bentgrass (Agrostis stolonifera) and to determine major ASM-responsive metabolites and proteins associated with enhanced abiotic stress tolerance. Creeping bentgrass plants (cv. ‘Penncross’) were foliarly sprayed with ASM and were exposed to non-stress (20/15°C day/night), heat stress (35/30°C), or drought conditions (by withholding irrigation) in controlled-environment growth chambers. Exogenous ASM treatment resulted in improved heat or drought tolerance, as demonstrated by higher overall turf quality, relative water content, and chlorophyll content compared to the untreated control. Western blotting revealed that ASM application resulted in up-regulation of ATP synthase, HSP-20, PR-3, and Rubisco in plants exposed to heat stress, and greater accumulation of dehydrin in plants exposed to drought stress. Metabolite profiling identified a number of amino acids, organic acids, and sugars which were differentially accumulated between ASM treated and untreated plants under heat or drought stress, including aspartic acid, glycine, citric acid, malic acid, and the sugars glucose, and fructose. Our results suggested that ASM was effective in improving heat or drought tolerance in creeping bentgrass, mainly through enhancing protein synthesis and metabolite accumulation involved in osmotic adjustment, energy metabolism, and stress signaling.

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“…In this context, there is evidence suggesting SA can influence the accumulation of some amino acids upon drought stress. A recent study focusing on drought in Creeping Bentgrass ( Agrostis stolonifera ) showed that SA conferred drought tolerance and also the accumulation of proline, serine, threonine and alanine (Li et al, 2016). However, an SA-influenced increased accumulation of carbohydrates, as noted in the Li et al (2016) study was not prominent in our results.…”

Section: Discussionmentioning

confidence: 99%

Linking Dynamic Phenotyping with Metabolite Analysis to Study Natural Variation in Drought Responses of Brachypodium distachyon

et al. 2016

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Drought is an important environmental stress limiting the productivity of major crops worldwide. Understanding drought tolerance and possible mechanisms for improving drought resistance is therefore a prerequisite to develop drought-tolerant crops that produce significant yields with reduced amounts of water. Brachypodium distachyon (Brachypodium) is a key model species for cereals, forage grasses, and energy grasses. In this study, initial screening of a Brachypodium germplasm collection consisting of 138 different ecotypes exposed to progressive drought, highlighted the natural variation in morphology, biomass accumulation, and responses to drought stress. A core set of ten ecotypes, classified as being either tolerant, susceptible or intermediate, in response to drought stress, were exposed to mild or severe (respectively, 15 and 0% soil water content) drought stress and phenomic parameters linked to growth and color changes were assessed. When exposed to severe drought stress, phenotypic data and metabolite profiling combined with multivariate analysis revealed a remarkable consistency in separating the selected ecotypes into their different pre-defined drought tolerance groups. Increases in several metabolites, including for the phytohormones jasmonic acid and salicylic acid, and TCA-cycle intermediates, were positively correlated with biomass yield and with reduced yellow pixel counts; suggestive of delayed senescence, both key target traits for crop improvement to drought stress. While metabolite analysis also separated ecotypes into the distinct tolerance groupings after exposure to mild drought stress, similar analysis of the phenotypic data failed to do so, confirming the value of metabolomics to investigate early responses to drought stress. The results highlight the potential of combining the analyses of phenotypic and metabolic responses to identify key mechanisms and markers associated with drought tolerance in both the Brachypodium model plant as well as agronomically important crops.

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Metabolic pathways regulated by abscisic acid, salicylic acid and γ‐aminobutyric acid in association with improved drought tolerance in creeping bentgrass (Agrostis stolonifera)

Cited by 160 publications

References 78 publications

Evidence for GABA-Induced Systemic GABA Accumulation in Arabidopsis upon Wounding

Evidence for GABA-Induced Systemic GABA Accumulation in Arabidopsis upon Wounding

Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass

Linking Dynamic Phenotyping with Metabolite Analysis to Study Natural Variation in Drought Responses of Brachypodium distachyon

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