Metabolic pathways for dietary lipids in the midgut of hematophagous Panstrongylus megistus (Hemiptera: Reduviidae)

Biochemical and cellular characterization of lipophorin–midgut interaction in the hematophagous Panstrongylus megistus (Hemiptera: Reduviidae)

“…3 demonstrated that the amount of bound lipophorin was dependent on the pH, being optimum around pH 6.8. This finding may be of physiological relevance since it was close to the pH range that we previously reported for the hemolymph of P. megistus (pH 6.5) (Canavoso et al, 2004a).…”

“…As most triatomines, this species takes large blood meals, abundant in lipids. During the digestion of dietary lipids the enterocytes experience a noticeable remodeling when they take up lipids from the lumen (Canavoso et al, 2004a). Storage of lipids in the enterocytes is transient, since they will be exported to circulating lipophorin.…”

“…Storage of lipids in the enterocytes is transient, since they will be exported to circulating lipophorin. Recently we demonstrated that both, phosphatidate phosphohydrolase and triacylglycerol lipase are important in regulating intracellular lipid synthesis and mobilization in the midgut of P. megistus (Canavoso et al, 2004a). However, exploration of the mechanisms mediating lipophorin binding at the midgut remains very scarce.…”

Analysis of the lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)

“…A high content of the oleic FA is recorded in the FBs of several other insects including the beetle Zophobas atratus (Howard & Stanley-Samuelson, 1996). Oleic acid is also the most common DG-lipophorin FA in the bloodsucking bug Panstrongylus megistus (Canavoso et al, 2004).…”

Oogenesis and egg development in triatomines: a biochemical approach

“…Lipophorin transports different classes of lipids, such as diacylglycerol, phospholipids, cholesterol, hydrocarbons, free fatty acids, between insect various tissues, according to physiological demand (Soulages and Wells 1994, Blacklock and Ryan 1994, Ryan and Van der Horst 2000. Hematophagous insects, such as the triatomines R. prolixus and Panstrongylus megistus, ingest large amounts of blood at each meal and, like it occurs in other insects, digestion causes the release of free fatty acids in midgut lumen which, after absorption, are used in the midgut epithelium for the synthesis of other lipids, as phospholipids, cholesteryl esthers, tri-and diacylglycerol (Tsuchida and Wells 1988, Turunen and Crailsheim 1996, Canavoso et al 2004. Lipids are then transferred to circulating lipophorin that transports them to the organs where they are stored and/or utilized , Coelho et al 1997, Canavoso et al 2004).…”