Metabolic Pathway of Guanidino Compounds in Chronic Renal Failure

Mikami, Hiroshi; Orita, Yoshimasa; Ando, Akio; Fujii, Masamitsu; Kikuchi, Takeo; Yoshihara, Kazuo; Okada, Akira; Abe, Hiroshi

doi:10.1007/978-1-4757-6903-6_55

Cited by 14 publications

(12 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). This is essentially the same result as in earlier reports [18,20,22] except for the possibility that GAA and arginine serve as a precursor of G in organs beside the liver as previously reported [18,19]. Regarding the origin of cana vanine, there are two possibilities: one is dietary because canavanine is identified in the seeds of a great number of leguminous plants [23] and the other is an endogenous synthesis via the 'guanidine cycle' as advocated by Natelson and Shirwin [24], However, the origin of canavanine re mains to be explored.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Biosynthesis of Guanidine in Isolated Rat Hepatocytes, Perfused Rat Liver and Intact Animals

Takemura

Nagase

Aoyagi

et al. 1994

Nephron

View full text Add to dashboard Cite

Plasma levels of guanidine (G) are reported to be increased in uremic patients and are synthesized from various guanidino compounds via a chemical reaction involving the hydroxyl radical in vitro. To identify both the metabolic precursor and the synthesizing organ of G, we investigated the concentrations of G in various organs of rats administered several guanidino compounds and we attempted to synthesize G biologically using isolated rat hepatocytes or perfused rat liver. In addition, we investigated the effect of the peroxidative state on the G synthesis in isolated hepatocytes using various reagents which alter this condition. Results show that the concentration of G increased in the kidney, liver and muscle following the administration of L-canavanine. In addition, G increased in the kidney at 90 min after the administration of guanidinoacetic acid (GAA). Moreover, G is synthesized from L-canavanine in isolated rat hepatocytes and perfused rat liver, and G synthesis in hepatocytes is partially inhibited by the addition of superoxide dismutase, catalase, glutathione or ethylenediaminetetraacetic acid. These results suggest that L-canavanine is possibly a biological precursor and GAA is an endogenous precursor of G. Furthermore, it is suggested that these reactions are closely related to the peroxidative state.

show abstract

Section: Discussionsupporting

confidence: 80%

“…With regard to a precursor of G in vivo, there are reports concerning the recovery of G in urine after the subcuta neous administration of arginine, canavanine [18] or GAA [19] to rats. But the pathway or organ of G synthesis in vivo has not been determined.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Guanidine in Isolated Rat Hepatocytes, Perfused Rat Liver and Intact Animals

Takemura

Nagase

Aoyagi

et al. 1994

Nephron

View full text Add to dashboard Cite

show abstract

“…One possible explanation of the difference is the existence of other organs besides the liver which may synthesize MG. The results of our in vivo experiment suggest that the kidney, lung, muscle, red blood cells and gut flora syn thesize MG, and it has been reported by others that gut flora [4] and muscle [10] synthesize MG from creatinine. But it is not proved whether other organs besides the liver synthesize MG or not.…”

Section: Discussionmentioning

confidence: 62%

“…With regard to the synthesizing organ, gut flora [4], liver [9,10] and muscle [10] are considered possibilities. However, these studies were long-term experiments in which creatinine or other possible precursors were ad ministered chronically or animals with chronic renal fai lure were used.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biosynthesis of Methylguanidine in Isolated Rat Hepatocytes and in vivo

Nagase

Aoyagi

Narita

et al. 1985

Nephron

View full text Add to dashboard Cite

To clarify the organ in which methylguanidine is synthesized, high doses of creatinine, which is known to stimulate the synthesis of methylguanidine, were administered to male Wistar rats intraperitoneally. Various tissues of the rats were frozen by a freeze clamp method before and 1, 2 and 3 h after injection, and methylguanidine was determined by high-pressure liquid chromatography using 9,10-phenanthrenequinone for fluorometric determination. We found evidence that the liver, kidney, lung, muscle, red blood cells and gut flora synthesize methylguanidine. In addition, we measured the synthesis of methylguanidine in isolated hepatocytes prepared from normal rats following the addition of creatinine, arginine and guanidinoacetic acid to the incubation medium. Synthesis of methylguanidine was observed only in those incubations which contained creatinine, and was dependent on the concentration of creatinine in the media and on the incubation period. Isolated rat hepatocytes also synthesized guanidine in the presence of guanidinoacetic acid. These results indicate that the liver is one of the organs which synthesize methylguanidine and also that creatinine is the precursor.

show abstract

Rapid analysis of guanidino compounds in serum from nephritic patients using column-switching with isocratic elution

Inamoto

Hanai³

et al. 1998

Biomed. Chromatogr.

View full text Add to dashboard Cite

Metabolic Pathway of Guanidino Compounds in Chronic Renal Failure

Cited by 14 publications

References 3 publications

Biosynthesis of Guanidine in Isolated Rat Hepatocytes, Perfused Rat Liver and Intact Animals

Biosynthesis of Guanidine in Isolated Rat Hepatocytes, Perfused Rat Liver and Intact Animals

Biosynthesis of Methylguanidine in Isolated Rat Hepatocytes and in vivo

Rapid analysis of guanidino compounds in serum from nephritic patients using column-switching with isocratic elution

Contact Info

Product

Resources

About