Metabolic pathway engineering for high-level production of 5-hydroxytryptophan in Escherichia coli

Wang, Haijiao; Liu, Wenqian; Shi, Feng; Huang, Lei; Lian, Jiazhang; Qin, Liang; Cai, Jin; Xu, Zhinan

doi:10.1016/j.ymben.2018.06.007

Cited by 39 publications

(74 citation statements)

References 32 publications

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“…Instead of using the native MH4 as the cofactor, Wang et al recently reconstituted the BH4 synthesis and regeneration pathways in an E. coli strain expressing a truncated version of human TPH2 (N∆145, C∆24), which had a significantly better soluble expression than that of the wild type (Wang et al, 2018a). The GCHI used in the BH4 synthesis was encoded by mtrA from B. subtilis, which improved BH4 biosynthesis, and the four other genes were human-sourced ( Figure 3b).…”

Section: Products Derived From the L-trp Branchmentioning

confidence: 99%

Building microbial factories for the production of aromatic amino acid pathway derivatives: From commodity chemicals to plant-sourced natural products

Cao

Gao

Suástegui

et al. 2020

Metabolic Engineering

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The aromatic amino acid biosynthesis pathway, together with its downstream branches, represents one of the most commercially valuable biosynthetic pathways, producing a diverse range of complex molecules with many useful bioactive properties. Aromatic compounds are crucial components for major commercial segments, from polymers to foods, nutraceuticals, and pharmaceuticals, and the demand for such products has been projected to continue to increase at national and global levels. Compared to direct plant extraction and chemical synthesis, microbial production holds promise not only for much shorter cultivation periods and robustly higher yields, but also for enabling further derivatization to improve compound efficacy by tailoring new enzymatic steps. This review summarizes the biosynthetic pathways for a large repertoire of commercially valuable products that are derived from the aromatic amino acid biosynthesis pathway, and it highlights both generic strategies and specific solutions to overcome certain unique problems to enhance the productivities of microbial hosts.

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Section: Products Derived From the L-trp Branchmentioning

confidence: 99%

Building microbial factories for the production of aromatic amino acid pathway derivatives: From commodity chemicals to plant-sourced natural products

Cao

Gao

Suástegui

et al. 2020

Metabolic Engineering

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“…The depletion of fossil fuel reserves, the economic problems associated with their use and the growing environmental concerns related with greenhouse gas emissions have led to a search for new renewable energy sources [1]. The use of lignocellulosic biomass for the production of bioethanol and value-added products has emerged as a sustainable alternative to fossil sources, as lignocellulose is one of the most abundantly available renewable biomass sources on earth and its utilization does not compete with the use of land for food production [2–4]. The complex and recalcitrant structure of lignocellulosic biomass comprises cellulose (a crystalline, linear homopolymer of glucose), hemicellulose (a branched, amorphous heteropolymer of hexoses and pentoses) and lignin [1].…”

Section: Introductionmentioning

confidence: 99%

Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways

Cunha

Soares

Romaní

et al. 2019

Biotechnol Biofuels

119

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Background: Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to Saccharomyces cerevisiae and tackle one of the major bottlenecks in the attainment of economically viable lignocellulosic ethanol production. Nevertheless, there is a lack of studies comparing the efficiency of those pathways both separately and combined. In this work, the XI and/or XR/ XDH pathways were introduced into two robust industrial S. cerevisiae strains, evaluated in synthetic media and corn cob hemicellulosic hydrolysate and the results were correlated with the differential enzyme activities found in the xylose-pathway engineered strains. Results: The sole expression of XI was found to increase the fermentative capacity of both strains in synthetic media at 30 °C and 40 °C: decreasing xylitol accumulation and improving xylose consumption and ethanol production. Similar results were observed in fermentations of detoxified hydrolysate. However, in the presence of lignocellulosicderived inhibitors, a positive synergistic effect resulted from the expression of both XI and XR/XDH, possibly caused by a cofactor equilibrium between the XDH and furan detoxifying enzymes, increasing the ethanol yield by more than 38%. Conclusions: This study clearly shows an advantage of using the XI from Clostridium phytofermentans to attain high ethanol productivities and yields from xylose. Furthermore, and for the first time, the simultaneous utilization of XR/ XDH and XI pathways was compared to the single expression of XR/XDH or XI and was found to improve ethanol production from non-detoxified hemicellulosic hydrolysates. These results extend the knowledge regarding S. cerevisiae xylose assimilation metabolism and pave the way for the construction of more efficient strains for use in lignocellulosic industrial processes.

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“…The flask yield was 100 mg/L in 60 h, and the batch fermentation yield was 962 mg/L (Mora-Villalobos and Zeng, 2018). Wang et al (2018) introduced human TPH into E. coli BL21, and co-expressed it with the synthetic and regenerative pathways of human BH 4 . The engineered bacteria hydroxylated 2 g/L trp to produce 1.24 g/L 5-HTP.…”

Section: Optimization Of Trp Supplymentioning

confidence: 99%

Advances in the Microbial Synthesis of 5-Hydroxytryptophan

Liu

Zhang

2021

Front. Bioeng. Biotechnol.

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5-Hydroxytryptophan (5-HTP) plays an important role in the regulation of emotion, behavior, sleep, pain, body temperature, and other physiological functions. It is used in the treatment of depression, insomnia, migraine, and other diseases. Due to a lack of effective biosynthesis methods, 5-HTP is mainly obtained by natural extraction, which has been unable to meet the needs of the market. Through the directed evolution of enzymes and the introduction of substrate supply pathways, 5-HTP biosynthesis and yield increase have been realized. This review provides examples that illustrate the production mode of 5-HTP and the latest progress in microbial synthesis.

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Metabolic pathway engineering for high-level production of 5-hydroxytryptophan in Escherichia coli

Cited by 39 publications

References 32 publications

Building microbial factories for the production of aromatic amino acid pathway derivatives: From commodity chemicals to plant-sourced natural products

Building microbial factories for the production of aromatic amino acid pathway derivatives: From commodity chemicals to plant-sourced natural products

Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways

Advances in the Microbial Synthesis of 5-Hydroxytryptophan

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