Metabolic Parameters in Wistar Rats Treated with Glucocorticosteroids and Vitamin E-Charged Poly Lactic-Co-Glycolic Acid (PLGA) Nanoparticles

Abstract

Glucocorticosteroids are the mainstay of treatment in many inflammatory and autoimmune disorders. Their administration can be associated with various side effects, similar to those within the metabolic syndrome. The beneficial effects of vitamin E in metabolic syndrome were demonstrated by many studies. Nowadays, vitamin E can be administrated through a new and innovative system delivery, such as polylactic-co-glycolic acid nanoparticles (PLGA-NPs). The aim of this experimental study was to compare the metabolic and haematological parameters after Prednisone administration as a single drug or in combination with vitamin E delivered by PLGA-NPs. Wistar male rats (n=15, age 10-12 months), on standard diet were randomised in three groups, for 6 weeks as: group SC served as a control, group SP was treated with Prednisone (1 mg/kg/day) by oral gavage and group SN received Prednisone (1 mg/kg/day) and vitamin E-charged PLGA nanoparticles (1 mg/kg/day) by oral gavage. In the Prednison treated group versus control, higher levels (p[0.05) were demonstrated for visceral fat weight, glycosylated hemoglobin (HbA1c), cholesterolemia and triglyceridemia. The association of PLGA-NPs charged with vitamin E to Prednisone prevented (p[0.05) hypercholesterolemia and visceral fat development induced by the cortisol intake. In conclusion, polylactic-co-glycolic acid nanoparticles (PLGA-NPs) charged with Vitamin E may demonstrate promising results in decreasing side effects induced by glucocorticosteroids, by reducing the amount of visceral fat and cholesterolemia.

Semicontinuous Fermentation of Cassava by Streptococcus bovis Using Membrane Bioreactor to Produce Lactic Acid

“…As can be seen in Table 1, dilution rate of 0.02 h -1 resulted in lactic acid with concentration of 19 gL -1 and yield of 0.75 gL -1 . The concentration of lactic acid achieved in this study is relatively higher than that reported in previous study [21], in which S. cremoris was used for fermentation of whey permeate with the concentration of 5.38 g L - 1 . In another study [22], production of lactic acid from cane molasses was investigated.…”

“…Lactic acid is recognized as a very valuable compound for its wide range of applications, from traditional uses such as chemical, soft drinks, medicine, and foodstuff, to more advanced materials such as biodegradable polymer and medical ingredients [1]. Both chemical route and fermentation process can be used to produce lactic acid; however the latter is more widely used for several advantageous reasons.…”

Analyze of Saliva Cortisol as Biomarker in Healthy Distressed Subjects

“…Chronic stress may lead to both increase and decrease in cortisol levels in general population [2,3]. Even though cortisol and its derivatives are beneficial as antiinflammatory drugs, and can be used in a variety of medical conditions [4,5], chronic emotional distress in young healthy adults persistently activates the HPA, this being translated into an exaggerated sympathetic response and cortisol increase at subjects exposed to general stressors [6,7].…”

The ocular protective effects of PLGA nanoparticles loaded with lutein, in fat diet Wistar rats, treated with sistemic glucocorticoids

“…This antioxidant reduced the elevated levels of ALT and AST enzymes in ethanol treated rats [24]. Also, it was demonstrated that PLGA-NPs loaded with vitamin E had visceral tissue protective effects and lowered cholesterolemia [25].…”