Metabolic output defines Escherichia coli as a health-promoting microbe against intestinal Pseudomonas aeruginosa

Christofi, Theodoulakis; Panayidou, Stavria; Dieronitou, Irini; Michael, Christina; Apidianakis, Yiorgos

doi:10.1038/s41598-019-51058-3

Cited by 34 publications

(30 citation statements)

References 61 publications

(63 reference statements)

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“…aeruginosa, though not a regular member of the physiological gut-microbiota, is frequently involved in intestinal infections and inflammatory conditions like colitis, gastroenteritis, and diarrhea. [39][40][41] P. aeruginosa secretes protein FapC, which self-assembles into functional amyloid fibrils to constitute the biofilm scaffold. [40,42] The Fap operon is also shared by other opportunistic gastric pathogens like Aeromonas caviae and Laribacter hongkongensis.…”

Section: Scheme Of Studymentioning

confidence: 99%

Accelerated Amyloid Beta Pathogenesis by Bacterial Amyloid FapC

et al. 2020

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The gut–brain axis has attracted increasing attention in recent years, fueled by accumulating symptomatic, physiological, and pathological findings. In this study, the aggregation and toxicity of amyloid beta (Aβ), the pathogenic peptide associated with Alzheimer's disease (AD), seeded by FapC amyloid fragments (FapCS) of Pseudomonas aeruginosa that colonizes the gut microbiome through infections are examined. FapCS display favorable binding with Aβ and a catalytic capacity in seeding the peptide amyloidosis. Upon seeding, twisted Aβ fibrils assume a much‐shortened periodicity approximating that of FapC fibrils, accompanied by a 37% sharp rise in the fibrillar diameter, compared with the control. The robust seeding capacity for Aβ by FapCS and the biofilm fragments derived from P. aeruginosa entail abnormal behavior pathology and immunohistology, as well as impaired cognitive function of zebrafish. Together, the data offer the first concrete evidence of structural integration and inheritance in peptide cross‐seeding, a crucial knowledge gap in understanding the pathological correlations between different amyloid diseases. The catalytic role of infectious bacteria in promoting Aβ amyloidosis may be exploited as a potential therapeutic target, while the altered mesoscopic signatures of Aβ fibrils may serve as a prototype for molecular assembly and a biomarker for screening bacterial infections in AD.

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Section: Scheme Of Studymentioning

confidence: 99%

Accelerated Amyloid Beta Pathogenesis by Bacterial Amyloid FapC

et al. 2020

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“…in the genera Buchnera and Sodalis). Many members of this family produce toxins called microcins [38], which may explain their ability to prevent mammalian gut colonization by pathogens [39,40]. In addition, they could directly benefit hosts by degrading complex dietary polysaccharides, or synthesizing limiting nutrients such as vitamins for the host.…”

Section: Plos Onementioning

confidence: 99%

The red flour beetle Tribolium castaneum: A model for host-microbiome interactions

Agarwal

Agashe

2020

PLoS ONE

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A large body of ongoing research focuses on understanding the mechanisms and processes underlying host-microbiome interactions, and predicting their ecological and evolutionary outcomes. To draw general conclusions about such interactions and understand how they are established, we must synthesize information from a diverse set of species. We analysed the microbiome of an important insect model-the red flour beetle Tribolium castaneumwhich is a widespread generalist pest of stored cereals. The beetles complete their entire life cycle in flour, which thus serves multiple functions: habitat, food, and a source of microbes. We determined key factors that shape the T. castaneum microbiome, established protocols to manipulate it, and tested its consequences for host fitness. We show that the T. castaneum microbiome is derived from flour-acquired microbes, and varies as a function of (flour) resource and beetle density. Beetles gain multiple fitness benefits from their microbiome, such as higher fecundity, egg survival, and lifespan; and reduced cannibalism. In contrast, the microbiome has a limited effect on development rate, and does not enhance pathogen resistance. Importantly, the benefits are derived only from microbes in the ancestral resource (wheat flour), and not from novel resources such as finger millet, sorghum, and corn. Notably, the microbiome is not essential for beetle survival and development under any of the tested conditions. Thus, the red flour beetle is a tractable model system to understand the ecology, evolution and mechanisms of host-microbiome interactions, while closely mimicking the host species' natural niche.

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“…Being a facultative anaerobe, E. coli helps creating an anaerobic environment by consuming the remaining oxygen in the gut [ 27 ]. It plays beneficial roles in human health by producing vitamin K [ 28 ] and conferring resistance to invading pathogens [ 29 , 30 ]. Despite its lower abundance in the human gut compared to several other major gut bacteria such as Bi fidobacterium, Bacteroides, Clostridium , etc., it is one of the most common gut colonizers [ 16 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Aspartame, acesulfame K and sucralose- influence on the metabolism of Escherichia coli

et al. 2020

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Background Gut microbes play a crucial role in the maintenance of human health. Components in the diet of the host affect their metabolism and diversity. Here, we investigated the influences of three commonly used non-caloric artificial sweeteners-aspartame, acesulfame K and sucralose-on the growth and metabolism of an omnipresent gut microbe Escherichia c oli K-12. Methods : Growth of E. coli in the presence of aspartame, acesulfame K and sucralose in media was assessed and the influences of these artificial sweeteners on metabolism were investigated by relative expression analysis of genes encoding the rate limiting steps of important metabolic pathways as well as their global metabolomic profiles. Results: As a whole, E. coli growth was inhibited by aspartame and induced by acesulfame potassium, while the effect of sucralose on growth was less prominent. Although the expressions of multiple key enzymes that regulate important metabolic pathways were significantly altered by all three sweeteners, acesulfame K caused the most notable changes in this regard. In multivariate analysis with the metabolite profiles, the sucralose-treated cells clustered the closest to the untreated cells, while the acesulfame potassium treated cells were the most distant. These sweeteners affect multiple metabolic pathways in E. coli , which include propanoate, phosphonate, phosphinate and fatty acid metabolism, pentose phosphate pathway, and biosynthesis of several amino acids including lysine and the aromatic amino acids. Similar to the gene expression pattern, acesulfame potassium treated E. coli showed the largest deviation in their metabolite profiles compared to the untreated cells.

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Metabolic output defines Escherichia coli as a health-promoting microbe against intestinal Pseudomonas aeruginosa

Cited by 34 publications

References 61 publications

Accelerated Amyloid Beta Pathogenesis by Bacterial Amyloid FapC

Accelerated Amyloid Beta Pathogenesis by Bacterial Amyloid FapC

The red flour beetle Tribolium castaneum: A model for host-microbiome interactions

Aspartame, acesulfame K and sucralose- influence on the metabolism of Escherichia coli

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