Metabolic Optimization by Enzyme-Enzyme and Enzyme-Cytoskeleton Associations

Araiza‐Olivera, Daniela; Uribe‐Carvajal, Salvador; Chiquete-Félix, Natalia; Rosas‐Lemus, Mónica; Granados, Gisela Ruíz; Sampedro, José G.; Mújica, Adela; Peña, Antonio

doi:10.5772/27813

Cited by 2 publications

(2 citation statements)

References 97 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Actin polymerization and turnover are energy dependent and essential for force generation by T cells. Actin polymerization requires ATP and some metabolic enzymes have been shown to be dynamically regulated by the cytoskeleton [121][122][123][124]. To fully elucidate the T-cell recognition mechanism, the role of forces generated and transduced by the cytoskeleton during antigen recognition needs to be fully addressed and understood at the molecular and cellular levels: one needs to understand how the multifunctional cytoskeleton provides active transportation of TCRs and other surface molecules, how the cytoskeleton uses energy to generate and transduce mechanical force, how the cytoskeleton works as an efficient machine to transduce signals in a fast manner, and how these processes are integrated together to facilitate T-cell recognition.…”

Section: Cytoskeleton Lipid and Receptor Nanostructurementioning

confidence: 99%

T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation

2019

View full text Add to dashboard Cite

A T cell is a sensitive self-referential mechanical sensor. Mechanical forces influence the recognition, activation, differentiation, and function throughout the lifetime of a T cell. T cells constantly perceive and respond to physical stimuli through their surface receptors, cytoskeleton, and subcellular structures. Surface receptors receive physical cues in the form of forces generated through receptor-ligand binding events, which are dynamically regulated by contact tension, shear stress, and substrate rigidity. The resulting mechanotransduction not only influences T-cell recognition and signaling but also possibly modulates cell metabolism and gene expression. Moreover, forces also dynamically regulate the deformation, organization, and translocation of cytoskeleton and subcellular structures, leading to changes in T-cell mobility, migration, and infiltration. However, the roles and mechanisms of how mechanical forces modulate T-cell recognition, signaling, metabolism, and gene expression, are largely unknown and underappreciated. Here, we review recent technological and scientific advances in T-cell mechanobiology, discuss possible roles and mechanisms of T-cell mechanotransduction, and propose new research directions of this emerging field in health and disease.

show abstract

Section: Cytoskeleton Lipid and Receptor Nanostructurementioning

confidence: 99%

T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation

2019

View full text Add to dashboard Cite

show abstract

“…A large and growing body of evidence also attests to the interaction of the cytoskeleton with a variety of metabolic enzymes [1] and references therein. It has been argued that the fundamental problem that confronts all cells is that of generating reproducible phenotypes on which natural selection can act [2].…”

Section: Introductionmentioning

confidence: 99%

Sensor potency of the moonlighting enzyme-decorated cytoskeleton: the cytoskeleton as a metabolic sensor

et al. 2013

View full text Add to dashboard Cite

BackgroundThere is extensive evidence for the interaction of metabolic enzymes with the eukaryotic cytoskeleton. The significance of these interactions is far from clear.Presentation of the hypothesisIn the cytoskeletal integrative sensor hypothesis presented here, the cytoskeleton senses and integrates the general metabolic activity of the cell. This activity depends on the binding to the cytoskeleton of enzymes and, depending on the nature of the enzyme, this binding may occur if the enzyme is either active or inactive but not both. This enzyme-binding is further proposed to stabilize microtubules and microfilaments and to alter rates of GTP and ATP hydrolysis and their levels.Testing the hypothesisEvidence consistent with the cytoskeletal integrative sensor hypothesis is presented in the case of glycolysis. Several testable predictions are made. There should be a relationship between post-translational modifications of tubulin and of actin and their interaction with metabolic enzymes. Different conditions of cytoskeletal dynamics and enzyme-cytoskeleton binding should reveal significant differences in local and perhaps global levels and ratios of ATP and GTP. The different functions of moonlighting enzymes should depend on cytoskeletal binding.Implications of the hypothesisThe physical and chemical effects arising from metabolic sensing by the cytoskeleton would have major consequences on cell shape, dynamics and cell cycle progression. The hypothesis provides a framework that helps the significance of the enzyme-decorated cytoskeleton be determined.

show abstract

Metabolic Optimization by Enzyme-Enzyme and Enzyme-Cytoskeleton Associations

Cited by 2 publications

References 97 publications

T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation

T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation

Sensor potency of the moonlighting enzyme-decorated cytoskeleton: the cytoskeleton as a metabolic sensor

Contact Info

Product

Resources

About