Metabolic network reconstruction of
            <i>Chlamydomonas</i>
            offers insight into light‐driven algal metabolism

Chang, Roger L.; Ghamsari, Lila; Manichaikul, Ani; Hom, Erik F. Y.; Balaji, S.; Fu, Weiqi; Shen, Yun; Hao, Tong; Palsson, Bernhard Ø.; Salehi‐Ashtiani, Kourosh; Papin, Jason A.

doi:10.1038/msb.2011.52

Cited by 271 publications

(273 citation statements)

References 60 publications

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“…Martineau et al [8] calculated that the amounts of dry matter per mole of artificial lighting gained by lettuce grown using red (650 nm) LEDs or highpressure sodium lamps were identical, and Chang et al [10] calculated that the maximum photon utilization efficiency for growth of the green alga Chlamydomonas reinhardtii under red LEDs is centred at 674 nm. Lettuce grown under red LEDs presented hypocotyls and cotyledons that were elongated, a phenomenon known to be phytochrome-dependent.…”

Section: Changing Light Intensity and Qualitymentioning

confidence: 99%

Photosynthesis under artificial light: the shift in primary and secondary metabolism

Darkó

Heydarizadeh

Schoefs

et al. 2014

Phil. Trans. R. Soc. B

330

208

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Providing an adequate quantity and quality of food for the escalating human population under changing climatic conditions is currently a great challenge. In outdoor cultures, sunlight provides energy (through photosynthesis) for photosynthetic organisms. They also use light quality to sense and respond to their environment. To increase the production capacity, controlled growing systems using artificial lighting have been taken into consideration. Recent development of light-emitting diode (LED) technologies presents an enormous potential for improving plant growth and making systems more sustainable. This review uses selected examples to show how LED can mimic natural light to ensure the growth and development of photosynthetic organisms, and how changes in intensity and wavelength can manipulate the plant metabolism with the aim to produce functionalized foods.

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Section: Changing Light Intensity and Qualitymentioning

confidence: 99%

Photosynthesis under artificial light: the shift in primary and secondary metabolism

Darkó

Heydarizadeh

Schoefs

et al. 2014

Phil. Trans. R. Soc. B

330

208

View full text Add to dashboard Cite

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“…Amino acid metabolism in iCZ843 contains 282 reactions and the total related reaction in other subsystems account for 312, compared with 34 reactions in C. protothecoides, 256 in C. reinhardtii, and 143 in Synechocystis spp. (Supplemental Table S4; Chang et al, 2011;Saha et al, 2011;Overbeek et al, 2014).…”

Section: Reconstructionmentioning

confidence: 99%

“…Using the genome annotation for C. vulgaris UTEX 395, as well as an existing and manually curated genome-scale model for C. reinhardtii (iRC1080; Chang et al, 2011), a preliminary draft reconstruction for C. vulgaris UTEX 395 based on protein homology was assembled.…”

Section: Reconstruction Of the C Vulgaris Utex 395 Metabolic Networkmentioning

confidence: 99%

“…As input, we provided the translated genome sequence of Chlorella vulgaris UTEX 395 and the manually curated reference network of the microalga Chlamydomonas reinhardtii (iRC1080; Chang et al, 2011), which was used as a reference network. The C. vulgaris UTEX 395 genome sequence was taken from the previously deposited sequence (GenBank no.…”

Section: Draft Generationmentioning

confidence: 99%

“…The predicted fluxes reveal the connectivity of Glu in the cytoplasm with the synthesis of several amino acids (Pro, His, Val, and Arg) in both light and dark conditions. Chloroplastic Glu is used by amino acid transferases (EC 2.6.1.42) for Chang et al (2011).…”

Section: Carbon and Amino Acid Metabolismmentioning

confidence: 99%

See 2 more Smart Citations

Genome-Scale Metabolic Model for the Green Alga Chlorella vulgaris UTEX 395 Accurately Predicts Phenotypes under Autotrophic, Heterotrophic, and Mixotrophic Growth Conditions

Zuñiga

Huelsman

et al. 2016

Plant Physiol.

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The green microalga Chlorella vulgaris has been widely recognized as a promising candidate for biofuel production due to its ability to store high lipid content and its natural metabolic versatility. Compartmentalized genome-scale metabolic models constructed from genome sequences enable quantitative insight into the transport and metabolism of compounds within a target organism. These metabolic models have long been utilized to generate optimized design strategies for an improved production process. Here, we describe the reconstruction, validation, and application of a genome-scale metabolic model for C. vulgaris UTEX 395, iCZ843. The reconstruction represents the most comprehensive model for any eukaryotic photosynthetic organism to date, based on the genome size and number of genes in the reconstruction. The highly curated model accurately predicts phenotypes under photoautotrophic, heterotrophic, and mixotrophic conditions. The model was validated against experimental data and lays the foundation for model-driven strain design and medium alteration to improve yield. Calculated flux distributions under different trophic conditions show that a number of key pathways are affected by nitrogen starvation conditions, including central carbon metabolism and amino acid, nucleotide, and pigment biosynthetic pathways. Furthermore, model prediction of growth rates under various medium compositions and subsequent experimental validation showed an increased growth rate with the addition of tryptophan and methionine.

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Transgenic Marine Microalgae: A Value‐Enhanced Fishmeal and Fish Oil Replacement

Gressel

2013

Handbook of Microalgal Culture

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Metabolic network reconstruction of Chlamydomonas offers insight into light‐driven algal metabolism

Abstract: A comprehensive genome-scale metabolic network of Chlamydomonas reinhardtii, including a detailed account of light-driven metabolism, is reconstructed and validated. The model provides a new resource for research of C. reinhardtii metabolism and in algal biotechnology.

Cited by 271 publications

References 60 publications

Photosynthesis under artificial light: the shift in primary and secondary metabolism

Photosynthesis under artificial light: the shift in primary and secondary metabolism

Genome-Scale Metabolic Model for the Green Alga Chlorella vulgaris UTEX 395 Accurately Predicts Phenotypes under Autotrophic, Heterotrophic, and Mixotrophic Growth Conditions

Transgenic Marine Microalgae: A Value‐Enhanced Fishmeal and Fish Oil Replacement

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