Metabolic modeling of the substrate competition among multiple VFAs for PHA production by mixed microbial cultures

Wang, Xiaofei; Carvalho, Gilda; Reis, Maria A.M.; Oehmen, Adrian

doi:10.1016/j.jbiotec.2018.06.342

Cited by 37 publications

(21 citation statements)

References 18 publications

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“…This result can be explained by the fact that HBut consumption is more energetically efficient than other SFPs for PHA production. 12,38 The theoretical amount of ATP required for the uptake of 1 Cmol of HBut is 1/2 mol, which is lower than that required for the uptake of HAce (1 mol) or HLac (2/3 mol). 38,39 Even though the literature is rich in studies where the strategies used in this work (uncoupled feeding strategy, high/ ideal OLR, and high HBut fraction) were tested individually, there are no examples where they were implemented together.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant

Matos

Cruz

Cardoso

et al. 2021

ACS Sustainable Chem. Eng.

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The full-scale implementation of polyhydroxyalkanoate (PHA) production using mixed microbial cultures (MMCs) has been limited by the low PHA global productivity and overall process yield. This work aimed to demonstrate, at a pilot scale, that by combining different effective operating conditions, it is possible to boost the PHA production performance when using fruit waste as a substrate. The organic loading rate (OLR) and pH of the acidogenic reactor were successfully used as tuning parameters to obtain a high fermentation yield (0.74 gCOD•gCOD −1 ) and a fermentate rich in butyrate, resulting in enhanced PHA production steps. A biomass highly enriched in PHA-storing microorganisms was selected as a result of uncoupling the carbon to the nitrogen feeding. The biomass concentration attained a notable value (7.83 g•L −1 ) as a response to the high OLR (8.7 gCOD•L −1 •d −1 ) imposed. In the PHA accumulation assays, the culture selected at the optimal OLR in the selection reactor achieved a high storage yield (0.98 gCOD•gCOD −1 ), and the continuous feeding strategy led to a maximum PHA content of 80.5% (g-basis). The high global productivity (8.1 g-PHA•L −1 •d −1 ) and overall process yield (0.45 gCOD•gCOD −1 ) are, to the best of the authors' knowledge, the highest values reported for MMC using a real feedstock at pilot scale. These results demonstrate the importance of combining different effective strategies to maximize the process performance, a promising result toward the full-scale implementation of PHA production from wastes and MMC.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…This result can be explained by the fact that HBut consumption is more energetically efficient than other SFPs for PHA production. , The theoretical amount of ATP required for the uptake of 1 Cmol of HBut is 1/2 mol, which is lower than that required for the uptake of HAce (1 mol) or HLac (2/3 mol). , …”

Section: Resultsmentioning

confidence: 99%

Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant

Matos

Cruz

Cardoso

et al. 2021

ACS Sustainable Chem. Eng.

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“…Struvite production was modeled in SUMO as a single CSTR with no solids recycle. Although metabolic models have been developed for aerobic dynamic feeding (ADF)‐driven PHA synthesis by mixed microbial consortia on waste streams (Dias et al, 2008; Wang, Carvalho, et al, 2018), to date, no model has been integrated into commercial process models. Instead, a simple stoichiometric approach was employed herein to model PHA production, consistent with data from Guho et al (2020) and using the DAIRIEES model (Guillen, 2017; Guillen et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Integrating dairy manure for enhanced resource recovery at a WRRF: Environmental life cycle and pilot‐scale analyses

Bryant¹,

Coats

2021

Water Environment Research

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The Twin Falls, Idaho wastewater treatment plant (WWTP), currently operates solely to achieve regulatory permit compliance. Research was conducted to evaluate conversion of the WWTP to a water resource recovery facility (WRRF) and to assess the WRRF environmental sustainability; process configurations were evaluated to produce five resources—reclaimed water, biosolids, struvite, biogas, and bioplastics (polyhydroxyalkanoates, PHA). PHA production occurred using fermented dairy manure. State‐of‐the‐art biokinetic modeling, performed using Dynamita's SUMO process model, was coupled with environmental life cycle assessment to quantify environmental sustainability. Results indicate that electricity production via combined heat and power (CHP) was most important in achieving environmental sustainability; energy offset ranged from 43% to 60%, thereby reducing demand for external fossil fuel‐based energy. While struvite production helps maintain a resilient enhanced biological phosphorus removal (EBPR) process, MgO2 production exhibits negative environmental impacts; integration with CHP negates the adverse consequences. Integrating dairy manure to produce bioplastics diversifies the resource recovery portfolio while maintaining WRRF environmental sustainability; pilot‐scale evaluations demonstrated that WRRF effluent quality was not affected by the addition of effluent from PHA production. Collectively, results show that a WRRF integrating dairy manure can yield a diverse portfolio of products while operating in an environmentally sustainable manner. Practitioner points Wastewater carbon recovery via anaerobic digestion with combined heat/power production significantly reduces water resource recovery facility (WRRF) environmental emissions. Wastewater phosphorus recovery is of value; however, struvite production exhibits negative environmental impacts due to MgO2 production emissions. Bioplastics production on imported organic‐rich agri‐food waste can diversify the WRRF portfolio. Dairy manure can be successfully integrated into a WRRF for bioplastics production without compromising WRRF performance. Diversifying the WRRF products portfolio is a strategy to maximize resource recovery from wastewater while concurrently achieving environmental sustainability.

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“…In addition, the experiment results of anaerobic carbon transformation (PHA/C) are consistent with the values predicted by the random model ( 7.1). The required energy for per C-mol of VFA conversion to corresponding acyl-CoA would decrease as the amount of carbon in the VFA increases (Oehmen et al 2005d, Wang et al 2018). Thus, P/C ratio of butyrate model is lower than that of acetate and propionate model.…”

Section: Anaerobic Metabolism Of Paomentioning

confidence: 99%

“…In general, the P-release and P/VFA ratio tended to decline with an increase in the chain length of the VFA added. The C4 and C5 carbon sources should require even less energy for active transport across the cell membrane as compared to C2 and C3 per C-mol of VFA uptake, as highlighted byWang et al (2018) for PHA producing mixed microbial cultures. In the case of Accumulibacter PAOs, similar results are obtained.…”

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confidence: 99%

The metabolism of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) system under the tropical climate

Wang¹

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Enhanced biological phosphorus removal (EBPR) system has been widely applied in wastewater plants due to the economy and sustainability virtue. However, high temperature (>30 ℃) enhanced biological phosphorus removal (EBPR) is challenging because glycogen accumulating organisms (GAOs) can easily outcompete polyphosphate accumulating organisms (PAOs) under high temperature conditions. The nature of the carbon source is also known to impact the PAO-GAO competition, though previous studies have not assessed how carbon source impacts PAO metabolism at high temperature. This study investigated the effects of different carbon sources on two acetate/propionate enriched Accumulibacter PAO cultures at high temperature and compared the performance of carbon transformation with low temperature EBPR cases reported in literature, and revealed several key metabolic differences. Besides the common volatile fatty acids (VFAs) such as acetate and propionate, PAOs also utilized butyrate and iso-butyrate, but hardly used valerate and its isomer, iso-valerate. When acetate and propionate are limited, butyrate and iso-butyrate could be used as supplementary carbon source for EBPR. In addition, PAOs, under high temperature, seemed to prefer propionate compared with other VFAs. Nevertheless, high aerobic glycogen replenishment was realized with propionate as the sole carbon source anaerobically, a trait not previously observed at low temperatures, which may be one of the reasons for EBPR failure in the long-term operation with propionate as feed. A combined substrate of acetate, propionate and perhaps butyrate seems to be a better carbon source choice since the total VFA uptake rate was the highest when they were

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Metabolic modeling of the substrate competition among multiple VFAs for PHA production by mixed microbial cultures

Cited by 37 publications

References 18 publications

Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant

Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant

Integrating dairy manure for enhanced resource recovery at a WRRF: Environmental life cycle and pilot‐scale analyses

The metabolism of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) system under the tropical climate

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