Metabolic mechanisms involved in hydroxylation reactions of diphenyl compounds by the lignin-degrading basidiomycete Phanerochaete chrysosporium

Hiratsuka, Nobuhiro; Oyadomari, Masafumi; Shinohara, Hiroaki; Tanaka, Hiroo; Wariishi, Hiroyuki

doi:10.1016/j.bej.2005.01.008

Cited by 22 publications

(19 citation statements)

References 33 publications

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“…Valli et al (1992) proposed a pathway for the oxidation of 2,7-DCDD by whole cultures or by purified LiP of P. chrysosporium ( Figure 5). Fungal P450 enzymes are probably also involved in the degradation of lignin and they may work together with peroxidases (Hiratsuka et al, 2005). Possibly, the degradation of chlorinated dioxins by WRF is initiated by P450 enzymes and extracellular enzymes are involved with later stages of degradation.…”

Section: Biodegradation Of Pcdd/fsmentioning

confidence: 99%

“…Aranda et al (2010) showed that APOs from A. aegerita and C. radians were able to oxidize non-chlorinated dibenzofuran to mono-, di-and tri-hydroxylated metabolites. Hiratsuka et al (2005) showed that purified LiP was able to degrade non-chlorinated dibenzop-dioxin. However, dibenzofuran was not degraded by purified LiP, although both dibenzo-pdioxin and dibenzofuran were metabolized by living mycelia of Phanerochaete chrysosporium.…”

Section: Degradation Of Pcdd/fs By Fungal Enzymes (Article Iii)mentioning

confidence: 99%

“…Nevertheless, in previous experiments non-chlorinated dioxins have be degraded or converted in liquid media by separated enzymes alone, such as peroxygenases (Aranda et al, 2010) or LiP (Hiratsuka et al, 2005). Aranda et al (2010) showed that APOs from A. aegerita and C. radians were able to oxidize non-chlorinated dibenzofuran to mono-, di-and tri-hydroxylated metabolites.…”

Section: Degradation Of Pcdd/fs By Fungal Enzymes (Article Iii)mentioning

confidence: 99%

“…In addition to extracellular LMEs, some WRF have also intracellular P450 (Hiratsuka et al, 2005;Stella et al, 2013). LMEs attack chlorinated dioxins cometabolically under aerobic conditions.…”

Section: Biodegradation Of Pcdd/fsmentioning

confidence: 99%

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Fungal enzyme production and biodegradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sawmill soil

et al. 2014

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Soils that are contaminated with the most recalcitrant organic contaminants, such as high molecular weight polyaromatic hydrocarbons (HMW PAH) and polychlorinated dibenzo-pdioxins and dibenzofurans (PCDD/F), cannot be degraded efficiently by conventional composting. The only available treatment method for these soils, which destroys the contaminants, is combustion at high temperature. This thesis examines three alternative fungal methods to treat these soils: 1) treatment with fungal enzymes, 2) treatment with fungal inoculum, and 3) fungal treatment used as a pre-treatment to improve the energy efficacy in combustion. Manganese peroxidase (MnP), which belongs to lignin-modifying enzymes (LME), was produced and used to treat PCDD/F-contaminated soil in the laboratory scale. Nevertheless, no degradation with a MnP preparation was observed, although a substantial amount of MnP activity was found in the soil still after 10 days of incubation. Both PAH-and PCDD/Fcontaminated soils were treated with fungal inoculum in the laboratory scale. HMW PAHs were degraded significantly more by the fungi than by the indigenous microbes alone in the laboratory experiments, where the PAH concentration of soil was 3500 mg kg -1 (sum of 16 PAH). Treatment with Phanerochaete velutina (inoculum) resulted to degradation of 96 % of 4-ring PAHs and 39 % of 5-and 6-ring PAHs in three months. With PCDD/F-contaminated soil, no degradation was observed in the control, but the degradation of PCDD/Fs with fungal treatments was significant (P. velutina: 62 %, Stropharia rugosoannulata: 64 % of WHO-TEQ value). Fungal treatment of PAH-contaminated soil was also applied in the field scale (2 t). However, both P. velutina (inoculum) and control treatment resulted in equal degradation in soil with lower PAH concentration (1400 mg kg -1 , sum of 16 PAH): 94 % of the 16 PAHs were degraded in three months. Fungal treatment was even applied as a pre-treatment for contaminated soil with high organic matter content, and which will be later combusted. In the pilot-scale (300 kg), 13 % degradation of the original organic matter content was obtained in 6 months. To conclude, fungal treatment is reasonable to apply for soils with organic contaminants that cannot be bioremediated by composting. With soils contaminated by chlorinated dioxins, this is always the case, but also PAH-contaminated soils with high total concentration or high proportion of HMW-PAHs. In addition, with fungal treatment the amount of organic matter in the soil can be reduced and the efficacy of the combustion process is improved. Tiivistelmä Kaikkein vaikeimmin hajoavilla orgaanisilla yhdisteillä kuten monirenkaisilla polyaromaattisilla hiilivedyillä (PAH) sekä polyklooratuilla dibenzo-p-dioksiineilla ja -furaaneilla (PCDD/F) pilaantuneita maita ei pystytä tehokkaasti kunnostamaan perinteisellä kompostoinnilla. Ainoa käytössä oleva puhdistusmenetelmä, joka hajottaa pilaavat yhdisteet, on maan poltto korkeassa lämpötilassa. Tämä väitöskirja esittelee kolme vaihtoehtoista valkolah...

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Section: Biodegradation Of Pcdd/fsmentioning

confidence: 99%

Section: Degradation Of Pcdd/fs By Fungal Enzymes (Article Iii)mentioning

confidence: 99%

Section: Degradation Of Pcdd/fs By Fungal Enzymes (Article Iii)mentioning

confidence: 99%

Section: Biodegradation Of Pcdd/fsmentioning

confidence: 99%

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Fungal enzyme production and biodegradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sawmill soil

et al. 2014

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“…Those biological chemical reactions can be used in order to introduce chiral centers, to resolve racemates, to convert a particular functional group among several groups with similar reactivities, to functionalize a nonactivated carbon regioselectively and to convert labile molecules due to the mild reaction conditions under which they take place [10]. A very good example are the ligninolytic enzymes from white-rot fungus Phanerochaete chrysosporium, that have been used in several biotransformation and bioremediation processes like the oxidation, by the extracellular enzyme lignin peroxidase (LiP) of diphenyl compounds biphenyl, biphenylene, dibenzofuran, dibenzop-dioxin and diphenyl ether, which constitute an important class of environmentally persistent pollutants [11]. More recently, the transformation of three anti-inflammatory drugs such as diclofenac, ibuprofen and naproxen were carried out by pellets of P. chrysosporium in fed-batch bioreactors operating under continuous air supply or periodic pulsation of oxygen [12].…”

Section: Introductionmentioning

confidence: 99%

Computational Modeling Methods for Understanding the Interaction of Lignin and Its Derivatives with Oxidoreductases as Biocatalysts

Alzate‐Morales¹,

Recabarren²,

Fuenzalida-Valdivia³

et al. 2018

Lignin - Trends and Applications

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This chapter will be presented as follow. First, a brief introduction to structure and characterization of lignin and its derivatives is presented, as well as their importance as chemical scaffolds for obtaining value-added products in chemical, food, pharmaceutical and agriculture industry. Second, an extensive review of different reports using computational modeling methods-like molecular dynamics simulations, quantum mechanics and hybrid calculation methods, among others-in the understanding of enzyme-substrate interaction and biocatalysis will be presented. Third, and as last part of chapter, some hand picked examples from literature will be chosen as successful cases where the interplay between experiment and computation has given as a result protein engineered oxidoreductases with improved catalytic capabilities.

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Degradation of Polycyclic Aromatic Hydrocarbons by Fungi

Cerniglia

Sutherland

2010

Handbook of Hydrocarbon and Lipid Microbiology

112

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Metabolic mechanisms involved in hydroxylation reactions of diphenyl compounds by the lignin-degrading basidiomycete Phanerochaete chrysosporium

Cited by 22 publications

References 33 publications

Fungal enzyme production and biodegradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sawmill soil

Fungal enzyme production and biodegradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sawmill soil

Computational Modeling Methods for Understanding the Interaction of Lignin and Its Derivatives with Oxidoreductases as Biocatalysts

Degradation of Polycyclic Aromatic Hydrocarbons by Fungi

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