Metabolic Mechanism of Mannan in a Ruminal Bacterium, Ruminococcus albus, Involving Two Mannoside Phosphorylases and Cellobiose 2-Epimerase

Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-β-d-mannosyl-d-glucose phosphorylase-like protein, UnkA

“…Similar mannandegradation systems have been found in the other CE producing bacteria, including Ruminococcus albus and Rhodothermus marinus. 5,6) In R. albus, β-(1→4)-mannooligosaccharides longer than β-(1→4)-mannobiose are phosphorolyzed to β-(1→4)-mannobiose and Man1P by the intracellular β-1,4-mannooligosaccharide phosphorylase (RaMP2) (EC 2.4.1.319). 5) Centeno et al 7) reported that a gene cluster belonging to the aerobic soil bacterium Cellvibrio mixtus (same strain as Cellvibrio vulgaris) was involved in the degradation of β-mannan.…”

Mannoside recognition and degradation by bacteria

“…A similar pathway has been identified in other mammal gut bacteria, like Ruminococcus albus 7, a ruminal anaerobic bacterium which efficiently degrades plant β-mannan using two synergistic GH130 mannoside phosphorylases (Kawahara et al, 2012). These two enzymes,…”

“…They are encoded together with mannoside-degrading enzymes by multigenic systems such as the polysaccharide utilization loci (PUL) characterized in Bacteroidetes and recently referenced in the PUL database , which is a highly useful resource to assess glycan catabolic functions in these organisms. Mannoside-specific PUL-like systems recently have been characterized, mainly in Bacteroides species but also in other bacteria (Martens, Chiang & Gordon, 2008;Sonnenburg et al, 2010;Martens et al, 2011;Senoura et al, 2011;Kawahara et al, 2012;McNulty et al, 2013;Abbott et al, 2015;Cuskin et al, 2015b). These genomic loci code for polysaccharide utilization systems which resemble the starch utilization system (Sus) found in Bacteroides thetaiotaomicron VPI-5482 (Reeves, Wang & Salyers, 1997;Shipman, Berleman & Salyers, 2000;Cho et al, 2001).…”

“…The human gut symbiont B. thetaiotaomicron VPI-5482 possesses a GH130 (Kawahara et al, 2012); (A2) plant N-glycan degradation by a Gram-negative bacterium such as Xanthomonas campestris pv. campestris (Dupoiron et al, 2015).…”

Characterization and crystal structure determination of β‐1,2‐mannobiose phosphorylase from Listeria innocua

“…S1) [5]. GH130_1 contains two 4-O-b-D-mannosyl-D-glucose phosphorylases (EC 2.4.1.281) from Bacteroides fragilis (MGP, locus tag BF0772) [6], and Ruminococcus albus (RaMP1, Rumal_0852) [7], and GH130_2 consists of enzymes with relatively relaxed specificities: b-1,4-mannooligosaccharide phosphorylase (EC 2.4.1.319) from R. albus (RaMP2, Rumal_0099) [7], 1,4-b-mannosyl-Nacetylglucosamine phosphorylase (EC 2.4.1.320) from Bacteroides thetaiotaomicron (BT1033) [8], and b-D-mannopyranosyl-1,4-N,N'-diacetylchitobiose phosphorylase (EC 2.4.1.-) from the human gut bacterial metagenome (UhgbMP) [5]. These enzymes are involved in the bacterial utilization of mannans or N-linked glycans.…”