Metabolic labeling probes for interrogation of the host–pathogen interaction

Ignacio, Bob J; Bakkum, Thomas; Bonger, Kimberly M.; Martin, Nathaniel I.; Kasteren, Sander I. van

doi:10.1039/d0ob02517h

Cited by 10 publications

(10 citation statements)

References 150 publications

(175 reference statements)

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“…In addition to imaging and profiling, chemical reporters provide exciting new opportunities for delivering diagnostic or therapeutic cargo specifically to pathogenic bacteria within an infected host, which is an especially attractive strategy due to the presence of numerous distinctive glycans in bacteria that are absent from mammals. This concept of modifying the bacterial cell surface for biomedical applications has been covered in reviews by the Spiegel and Dube groups, with the latter focusing specifically on the targeting of pathogen-specific bacterial glycans. , Other recent reviews have also highlighted how various chemical biology approaches, including the use of metabolic labeling and bioorthogonal chemistry, can be enlisted to advance the broader bacteriology field. ,,,− …”

Section: Introductionmentioning

confidence: 99%

Chemical Reporters for Bacterial Glycans: Development and Applications

2021

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Bacteria possess an extraordinary repertoire of cell envelope glycans that have critical physiological functions. Pathogenic bacteria have glycans that are essential for growth and virulence but are absent from humans, making them high-priority targets for antibiotic, vaccine, and diagnostic development. The advent of metabolic labeling with bioorthogonal chemical reporters and small-molecule fluorescent reporters has enabled the investigation and targeting of specific bacterial glycans in their native environments. These tools have opened the door to imaging glycan dynamics, assaying and inhibiting glycan biosynthesis, profiling glycoproteins and glycan-binding proteins, and targeting pathogens with diagnostic and therapeutic payload. These capabilities have been wielded in diverse commensal and pathogenic Gram-positive, Gram-negative, and mycobacterial speciesincluding within live host organisms. Here, we review the development and applications of chemical reporters for bacterial glycans, including peptidoglycan, lipopolysaccharide, glycoproteins, teichoic acids, and capsular polysaccharides, as well as mycobacterial glycans, including trehalose glycolipids and arabinan-containing glycoconjugates. We cover in detail how bacteria-targeting chemical reporters are designed, synthesized, and evaluated, how they operate from a mechanistic standpoint, and how this information informs their judicious and innovative application. We also provide a perspective on the current state and future directions of the field, underscoring the need for interdisciplinary teams to create novel tools and extend existing tools to support fundamental and translational research on bacterial glycans.

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Section: Introductionmentioning

confidence: 99%

Chemical Reporters for Bacterial Glycans: Development and Applications

2021

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“…The AIE photosensitizer can be expressed on the bacterial surface via the D-alanine metabolic pathway, enabling real-time imaging and in situ photodynamic ablation of intracellular pathogens. 82,84 Metabolic labeling-based bio-orthogonal reactions have also been demonstrated to improve the targeting capability of therapeutic nanoparticles, enhancing the thermal killing efficiency for pathogenic bacteria. 29 By extending natural surface ligands to metabolic chemical analogs, diverse artificial ligands can be incorporated onto the bacterial surface without interfering with native biological processes, vastly expanding the capability for bacterial surface engineering.…”

Section: Artificial Surface Ligands and Interactionsmentioning

confidence: 99%

Engineering bacterial surface interactions using DNA as a programmable material

Kong

et al. 2022

Chem. Commun.

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“…The fluorescent probes are directly labeled on the surface of bacteria through the unique metabolic pathway. The indirect labeling method involves the use of metabolic precursors modified by biological orthogonal functional groups (such as alkyne and azide) to culture bacteria through click chemistry on the bacteria for fluorescence labeling [ 21 , 22 ]. The bacterial surface contains abundant metabolic compounds, among which peptidoglycan stem peptide, outer-membrane glycolipids, pseudoamino, and maltose are commonly used in metabolic labeling of pathogenic bacteria.…”

Section: Metabolic Labeling Of the Surface Components Of Pathogenic B...mentioning

confidence: 99%

Recent Progress in Identifying Bacteria with Fluorescent Probes

Wang

et al. 2022

Molecules

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The development of new techniques to rapidly and accurately detect bacteria has drawn continuous attention due to the potential threats posed by bacteria to human health and safety. Recently, a novel strategy based on fluorescent probes has drawn considerable interest for the detection of bacteria due to its high selectivity, fast response, and simple operation. In this review, we summarize the recent progress on fluorescent probes for the specific recognition and discrimination of Gram-negative and Gram-positive bacteria. In particular, we outline current design strategies, such as targeting of the differences in surface components, cell wall components, endogenous enzymes, surface charge, and hydrophobicity of various kinds of bacteria to develop various fluorescent sensors (organic small-molecule fluorescent probes, nanoprobes, and metal ion probes). We also emphasize the application of organic molecules in probe recognition elements. We hope that this review can stimulate this research area in bacterial detection and imaging in the future.

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Metabolic labeling probes for interrogation of the host–pathogen interaction

Abstract: Metabolic labeling of intracellular pathogens can provide new methods of studying host pathogen interactions.

Cited by 10 publications

References 150 publications

Chemical Reporters for Bacterial Glycans: Development and Applications

Chemical Reporters for Bacterial Glycans: Development and Applications

Engineering bacterial surface interactions using DNA as a programmable material

Recent Progress in Identifying Bacteria with Fluorescent Probes

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