“…Copper(I)-catalyzed azide-alkyne cycloaddition, CuAAC, can be used to detect alkynes using azides or vice versa ( Rostovtsev et al, 2002 ; Tornoe et al, 2002 ). Applying this technique to lipids, including sterols, has yielded numerous examples, where clickable lipids bearing an alkyne or azido tag were successfully used to monitor protein lipidation, protein–lipid interaction, lipid localization and metabolism, and this work has been extensively reviewed ( Haberkant and Holthuis, 2014 ; Kuerschner and Thiele, 2014 ; Jao et al, 2015 ; Bumpus and Baskin, 2018 ; Laguerre and Schultz, 2018 ; Ancajas et al, 2020 ; Schoop et al, 2021 ).…”