Metabolic interaction of hydrogen peroxide and hypoxia in zebrafish fibroblasts

Dikova, Valentina; Vorhauser, Julia; Geng, Anne; Pelster, Bernd; Sandbichler, Adolf Michael

doi:10.1016/j.freeradbiomed.2019.11.015

Cited by 6 publications

(1 citation statement)

References 72 publications

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“…First, the amount of catalase, which is responsible for H 2 O 2 degradation, did not change between age groups. Second, it has recently been shown that a considerable portion of the ECAR of Z3 cells derives from CO 2 from the pentose phosphate pathway (PPP) [36], which is the main recycler of the redox currency NADPH. Since the basal ECAR of cells supplied with glucose did not significantly change between the different age groups (Fig.…”

Section: Discussionmentioning

confidence: 99%

Aging Cell Culture - Genetic and Metabolic Effects of Passage Number on Zebrafish Z3 Cells

Šrut

Peer

Fiechtner

et al. 2022

Cell Physiol Biochem

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BACKGROUND/AIMS: Since cell lines are cultured and extensively used in a variety of different research disciplines, we determined the effects of passage numbers on a commonly used embryonic zebrafish cell line (Z3). METHODS: Senescence markers, DNA damage, the redox state, gene expression, and metabolic parameters have been investigated in young (passage 5) up to very old (passage 40 and higher) cells. RESULTS: Besides increasing DNA damage, we also found elevated metabolic capacity and a shift to a more reduced cellular redox state in the cells. Interestingly, several parameters showed a non-linear course regarding the passage number or cell age, so that for example young and mid-aged cells appeared to cluster with very old rather than with old cells. CONCLUSION: This study illustrates the importance of passage number and suggests pre-testing specific parameters to assure the generation of accurate and reproducible data.

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Section: Discussionmentioning

confidence: 99%