Metabolic interaction at the level of extracellular amino acids between plant growth-promoting rhizobacteria and plants of alfalfa (Medicago sativa L.)

Use of a Consortium of Agronomically Important Microorganisms for Growing Soybean (Glycine max (L.) Merr.)

“…Rhizosphere soil samples were collected in sterile bags in compliance with the rules of asepsis. The isolation of phosphate-solubilizing bacteria was carried out as follows: 10 g of a soil sample was crushed and placed in 10 ml of sterile saline solution (0.9% NaCl) [40]. Then, we prepared dilutions from 10 -1 to 10 -6 and sowed them in Petri dishes with an NBRIP (National Botanical Research Institute's phosphate growth medium) agar medium with the following composition, g/l: glucose: 10.0, Ca 3 (PO 4 ) 2 : 5.0, MgCl 2 ×6H 2 O: 5.0, MgSO 4 ×7H 2 O: 0.25, KCl: 0.2, (NH 4 ) 2 SO 4 : 0.1 [41].…”

“…The dishes were incubated at 28°C until transparent zones (halo zones) appeared in the environment around bacterial colonies (2-3 days). Pure bacterial cultures were obtained by picking individual colonies with the help of an inoculation loop [40]. The bacteria were cultured on a liquid and NBRIP agar medium on a shaker at 180 rpm and a temperature of 28°C.…”

“…Sterile tap water was used to water the plants. The negative control was seeds treated with sterile water [40].…”

“…Plants were grown in a climate chamber (Memmert HPP 750 Constant Climate Chamber, Germany) under the following conditions: daylight: 12 hours, temperature: 25°C, illumination: cold white light at 6,500 K, warm light at 2,700 K; night mode: 12 hours; temperature 15°C, humidity: 65% [40].…”

“…Agrochemical indicators were humus 1.2%, easily hydrolyzed nitrogen: 58.2 mg/kg of soil, mobile phosphorus: 21.4 mg/kg of soil, and mobile potassium: 460 mg/kg. The reaction nature of the soil was slightly alkaline (pH 7.8) [40]. In the experiments, we used consortiums that included rhizobia strains (Bradyrhizobium lupini RH-6; Br.…”