Metabolic impacts of polystyrene microplastics on the freshwater microalga Microcystis aeruginosa

Fan, Yifan; Liu, Tong; Qian, Xin; Deng, Ligang; Rao, Wenxin; Zhang, Qiji; Zheng, Jinglan; Gao, Xiang

doi:10.1016/j.scitotenv.2022.155655

Cited by 19 publications

(2 citation statements)

References 38 publications

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“…Furthermore, the correlation between an MPs treatment and alterations in arginine levels has only been reported in a few studies. A significant disruption in arginine-related pathways was observed in Microcystis aeruginosa, a common freshwater microalga, after an MPs (0.1, 1, 10, and 100 µm) exposure for 96 h [49]. Therefore, these findings provide novel evidence that arginine-related pathways could be considered an important target while assessing the impact of MPs on liver metabolism.…”

Section: Discussionmentioning

confidence: 62%

Impact of the Oral Administration of Polystyrene Microplastics on Hepatic Lipid, Glucose, and Amino Acid Metabolism in C57BL/6Korl and C57BL/6-Lepem1hwl/Korl Mice

Roh,

Kim,

Song

et al. 2024

IJMS

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The impact of microplastics (MPs) on the metabolic functions of the liver is currently unclear and not completely understood. To investigate the effects of the administration of MPs on the hepatic metabolism of normal and obese mice, alterations in the lipid, glucose (Glu), and amino acid regulation pathways were analyzed in the liver and adipose tissues of C57BL/6Korl (wild type, WT) or C57BL/6-Lepem1hwl/Korl mice (leptin knockout, Lep KO) orally administered polystyrene (PS) MPs for 9 weeks. Significant alterations in the lipid accumulation, adipogenesis, lipogenesis, and lipolysis pathways were detected in the liver tissue of MP-treated WT and Lep KO mice compared to the vehicle-treated group. These alterations in their liver tissues were accompanied by an upregulation of the serum lipid profile, as well as alterations in the adipogenesis, lipogenesis, and lipolysis pathways in the adipose tissues of MP-treated WT and Lep KO mice. Specifically, the level of leptin was increased in the adipose tissues of MP-treated WT mice without any change in their food intake. Also, MP-induced disruptions in the glycogenolysis, Glu transporter type 4 (GLUT4)-5′ AMP-activated protein kinase (AMPK) signaling pathway, levels of lipid intermediates, and the insulin resistance of the liver tissues of WT and Lep KO mice were observed. Furthermore, the levels of seven endogenous metabolites were remarkably changed in the serum of WT and Lep KO mice after MP administrations. Finally, the impact of the MP administration observed in both types of mice was further verified in differentiated 3T3-L1 adipocytes and HepG2 cells. Thus, these results suggest that the oral administration of MPs for 9 weeks may be associated with the disruption of lipid, Glu, and amino acid metabolism in the liver tissue of obese WT and Lep KO mice.

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Section: Discussionmentioning

confidence: 62%

Impact of the Oral Administration of Polystyrene Microplastics on Hepatic Lipid, Glucose, and Amino Acid Metabolism in C57BL/6Korl and C57BL/6-Lepem1hwl/Korl Mice

Roh,

Kim,

Song

et al. 2024

IJMS

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“…Cultures were incubated for 96 h at 25 °C with a 12:12 light–dark cycle. The 96 h exposure duration used in this study is a typical exposure duration in toxicological research that is widely applied in previous studies. ,− The flasks were shaken three times a day …”

Section: Methodsmentioning

confidence: 99%

Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

Mao,

Ye,

Yang

et al. 2024

Environ. Sci. Technol.

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Antibiotics are being increasingly detected in aquatic environments, and their potential ecological risk is of great concern. However, most antibiotic toxicity studies involve single-exposure experiments. Herein, we studied the effects and mechanisms of repeated versus single clarithromycin (CLA) exposure on Microcystis aeruginosa. The 96 h effective concentration of CLA was 13.37 μg/L upon single exposure but it reduced to 6.90 μg/L upon repeated exposure. Single-exposure CLA inhibited algal photosynthesis by disrupting energy absorption, dissipation and trapping, reaction center activation, and electron transport, thereby inducing oxidative stress and ultrastructural damage. In addition, CLA upregulated glycolysis, pyruvate metabolism, and the tricarboxylic acid cycle. Repeated exposure caused stronger inhibition of algal growth via altering photosynthetic pigments, reaction center subunits biosynthesis, and electron transport, thereby inducing more substantial oxidative damage. Furthermore, repeated exposure reduced carbohydrate utilization by blocking the pentose phosphate pathway, consequently altering the characteristics of extracellular polymeric substances and eventually impairing the defense mechanisms of M. aeruginosa. Risk quotients calculated from repeated exposure were higher than 1, indicating significant ecological risks. This study elucidated the strong influence of repeated antibiotic exposure on algae, providing new insight into antibiotic risk assessment.

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Liquid Chromatography/Mass Spectrometry in Environmental Analysis

Mattarozzi

Careri

2023

Encyclopedia of Analytical Chemistry

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Liquid chromatography‐mass spectrometry (LC‐MS) has expanded the analytical framework for the analysis of polar organic contaminants in complex matrices primarily through advances in high‐resolution mass spectrometry, ion mobility spectrometry, evolving repository databases, and data mining software. Over the last 10 years, the analytical trends toward a comprehensive characterization of emerging environmental contaminants along with their degradation and transformation products have driven the move from target analysis of few tens of compounds to wide‐scope target screening, as well as the development of strategies aimed at suspect screening of ‘known unknowns’ and the more challenging nontarget analysis for the identification of ‘unknown unknowns’. In this context, there is also an urgent need to give objective and unambiguous evidence of the confidence level of identification from a large list of tentatively identified substances, as well as to automate the evaluation process toward a more reproducible and harmonized approach. This article represents a third edition of a previous manuscript by Mattarozzi and Careri, (1) which was updated to take into account the huge progress in MS instrumentation and related methodologies and also advances in environmental analytical chemistry. In this article, among the environmental applications of LC‐MS, current and innovative approaches for the analysis of selected classes of emerging pollutants, such as micro‐ and nanoplastics, per‐ and poly‐fluoroalkyl substances, and transformation products in environmental samples are discussed, attempting to have a global coverage on the most recent research studies regarding the development of methodologies for the contaminants of high concern for the environment.

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Metabolic impacts of polystyrene microplastics on the freshwater microalga Microcystis aeruginosa

Cited by 19 publications

References 38 publications

Impact of the Oral Administration of Polystyrene Microplastics on Hepatic Lipid, Glucose, and Amino Acid Metabolism in C57BL/6Korl and C57BL/6-Lepem1hwl/Korl Mice

Impact of the Oral Administration of Polystyrene Microplastics on Hepatic Lipid, Glucose, and Amino Acid Metabolism in C57BL/6Korl and C57BL/6-Lepem1hwl/Korl Mice

Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

Liquid Chromatography/Mass Spectrometry in Environmental Analysis

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