Metabolic Footprints of Burkholderia Sensu Lato Rhizosphere Bacteria Active against Maize Fusarium Pathogens

Abstract

Consistent with their reported abundance in soils, several Burkholderia sensu lato strains were isolated from the rhizosphere of maize plants cultivated at different sites in central México. Comparative analysis of their 16S rRNA gene sequences permitted their separation into three distinctive clades, which were further subdivided into six other clusters by their close resemblance to (1) Trinickia dinghuensis; (2) Paraburkholderia kirstenboschensis, P. graminis, P. dilworthii and P. rhynchosiae; (3) B. gladioli; (4) B. arboris; (5) B. contaminans, or (6) B. metallica representative species. Direct confrontation assays revealed that these strains inhibited the growth of pathogenic Fusarium oxysporum f. sp. radicis-lycopersici, and F. verticillioides within a roughly 3–55% inhibition range. The use of a DIESI-based non-targeted mass spectroscopy experimental strategy further indicated that this method is an option for rapid determination of the pathogen inhibitory capacity of Burkholderia sensu lato strains based solely on the analysis of their exometabolome. Furthermore, it showed that the highest anti-fungal activity observed in B. contaminans and B. arboris was associated with a distinctive abundance of certain m/z ions, some of which were identified as components of the ornbactin and pyochelin siderophores. These results highlight the chemical diversity of Burkholderia sensu lato bacteria and suggest that their capacity to inhibit the Fusarium-related infection of maize in suppressive soils is associated with siderophore synthesis.

Traditional Chinese medicine residues promote the growth and quality of Salvia miltiorrhiza Bunge by improving soil health under continuous monoculture

“…The other is that the relative abundance of some beneficial bacteria significantly increased in the soils of the TCMR treatments, including Burkholderia , Sphingobium , and Rhizobium , which were significantly negatively correlated with the abundance of Fusarium ( Figure 9 ), suggesting that they may inhibit the growth of Fusarium . Burkholderia is the most commonly reported genus of bacteria with antagonistic activity against Fusarium and inhibits Fusarium diseases in many plants, including maize, tomatoes, and bananas ( Abdel-Aziz et al., 2020 ; Barrera-Galicia et al., 2021 ). Beneficial microorganisms may shield plants from diseases by altering the plant immune system ( Liu et al., 2021 ).…”

BIODIVERSIDADE DE BACTÉRIAS ANTAGÔNICAS A FUNGOS ASSOCIADOS À SINDROME DA MORTE DO CAPIM-MARANDU (Brachiaria brizantha)

“…Quando consideramos antagonismo dos gêneros de bactérias obtidos no presente trabalho é possível citar alguns exemplos descritos na literatura como: Burkholderia antagônica à Fusarium spp., patógeno de gramíneas (LUVIZOTTO, 2008;BARRERA-GALICIA et al 2021 1-F: floresta; 2-L: lavoura; 3-P: pecuária; 4-IPL: integração pecuária-lavoura; 5-iLP: integração lavoura-pecuária; 6-iLF: integração lavoura-floresta; 8-ILPF: lavoura e floresta por 2 anos e após pecuária e floresta por 2 anos; 9ILPF: pecuária e floresta por 2 anos e lavoura e floresta por 2 anos; 10-ILPF: integração lavoura pecuária floresta. *Similaridade.…”

His-Ala-Phe-Lys peptide from Burkholderia arboris possesses antifungal activity

“…Burkholderia arboris has been widely isolated from the rhizosphere zone of the soil, and has been shown to possess antifungal activity (Barrera-Galicia et al, 2021); however, the antifungal compound is yet to be identified.…”

“…Burkholderia cepacia complex (BCC) is a group of antibiotic resistant bacteria, including Burkholderia arboris , capable of producing bioactive compounds via NRPS and PKS ( Thomson and Dennis, 2012 ). Burkholderia arboris has been widely isolated from the rhizosphere zone of the soil, and has been shown to possess antifungal activity ( Barrera-Galicia et al, 2021 ); however, the antifungal compound is yet to be identified.…”