Metabolic Evaluation of Cryopreserved Corneal Tissue

Geroski, Dayle H.; Edelhauser, Henry F.

doi:10.1001/archopht.1974.03900060136011

Cited by 9 publications

(2 citation statements)

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“…Lack of DMSO toxicity to human endothelium at 4°C in albumin plus sucrose was found by Schultz (197 1) and by Kanai et al (1972). Parallel observations on rabbit endothelium have been reported by Kaufman & Capella (1968), van Horn et al (1972, McCary et al (1973), Geroski & Edelhauser (1974 and by Basta et al (1975). Lack of DMSO toxicity to human endothelium at 2"C, in a medium comparable to MEM, was found by Mueller (1 968).…”

Section: Commentssupporting

confidence: 70%

Toxicity of Dimethylsulfoxide (Dmso) to Human Corneal Endothelium in Vitro

Sperling

Larsen

1979

Acta Ophthalmologica

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Human cadaver corneas were obtained 10--12 h after clinical death, incubated in organ culture for 20--28 h and exposed 2, 4, 6, 8, 6, 4, and 2% DMSO successively for 10 min at each concentration. Endothelial cell damage was recognized by morphological alterations during reformation of a coherent cell sheat after expulsion of damaged cells. DMSO was found to cause latent cell damage at 31 degrees C. No cell damage was found at 4 degrees C when DMSO was dissolved in minimum essential medium (Eagle) plus Dextran or when DMSO was dissolved in human albumin plus sucrose. DMSO was found to be toxic at 4 degrees C in pure serum and in Dulbecco's medium.

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