Metabolic Evaluation of Cryopreserved Corneal Tissue

Toxicity of Dimethylsulfoxide (Dmso) to Human Corneal Endothelium in Vitro

“…Lack of DMSO toxicity to human endothelium at 4°C in albumin plus sucrose was found by Schultz (197 1) and by Kanai et al (1972). Parallel observations on rabbit endothelium have been reported by Kaufman & Capella (1968), van Horn et al (1972, McCary et al (1973), Geroski & Edelhauser (1974 and by Basta et al (1975). Lack of DMSO toxicity to human endothelium at 2"C, in a medium comparable to MEM, was found by Mueller (1 968).…”

Toxicity of Dimethylsulfoxide (Dmso) to Human Corneal Endothelium in Vitro

“…Lack of DMSO toxicity to human endothelium at 4°C in albumin plus sucrose was found by Schultz (197 1) and by Kanai et al (1972). Parallel observations on rabbit endothelium have been reported by Kaufman & Capella (1968), van Horn et al (1972, McCary et al (1973), Geroski & Edelhauser (1974 and by Basta et al (1975). Lack of DMSO toxicity to human endothelium at 2"C, in a medium comparable to MEM, was found by Mueller (1 968).…”

Effect of postmortem interval on the Graft Endothelium During Preservation and After Transplantation for Keratoconus

“…It is observed that the metabolism of the cells get reduced by 13% when the corneas are cryopreserved compared with fresh tissues. 17 Therefore, metabolic evaluation could be a further important investigative step to understand if the postmortem interval has any effect on the metabolic activities of the cornea preserved in either organ culture, hypothermic storage, or even cryopreserved in specific cases.…”

Toxicity of Dimethylsulfoxide (Dmso) to Human Corneal Endothelium in Vitro

“…Lack of DMSO toxicity to human endothelium at 4°C in albumin plus sucrose was found by Schultz (197 1) and by Kanai et al (1972). Parallel observations on rabbit endothelium have been reported by Kaufman & Capella (1968), van Horn et al (1972, McCary et al (1973), Geroski & Edelhauser (1974 and by Basta et al (1975). Lack of DMSO toxicity to human endothelium at 2"C, in a medium comparable to MEM, was found by Mueller (1 968).…”

Effect of postmortem interval on the Graft Endothelium During Preservation and After Transplantation for Keratoconus

“…It is observed that the metabolism of the cells get reduced by 13% when the corneas are cryopreserved compared with fresh tissues. 17 Therefore, metabolic evaluation could be a further important investigative step to understand if the postmortem interval has any effect on the metabolic activities of the cornea preserved in either organ culture, hypothermic storage, or even cryopreserved in specific cases.…”

Toxicity of Dimethylsulfoxide (Dmso) to Human Corneal Endothelium in Vitro

“…Lack of DMSO toxicity to human endothelium at 4°C in albumin plus sucrose was found by Schultz (197 1) and by Kanai et al (1972). Parallel observations on rabbit endothelium have been reported by Kaufman & Capella (1968), van Horn et al (1972, McCary et al (1973), Geroski & Edelhauser (1974 and by Basta et al (1975). Lack of DMSO toxicity to human endothelium at 2"C, in a medium comparable to MEM, was found by Mueller (1 968).…”

Effect of postmortem interval on the Graft Endothelium During Preservation and After Transplantation for Keratoconus

“…It is observed that the metabolism of the cells get reduced by 13% when the corneas are cryopreserved compared with fresh tissues. 17 Therefore, metabolic evaluation could be a further important investigative step to understand if the postmortem interval has any effect on the metabolic activities of the cornea preserved in either organ culture, hypothermic storage, or even cryopreserved in specific cases.…”