Metabolic enzyme(s) confer imidacloprid resistance in a clone of <i>Myzus persicae</i> (Sulzer) (Hemiptera: Aphididae) from Greece

Philippou, Despina; Field, L. M.; Moores, G. D.

doi:10.1002/ps.1888

Cited by 71 publications

(73 citation statements)

References 28 publications

(42 reference statements)

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“…A previous study examining the susceptibility of the 5191A clone to the pyrethroid α-cypermethrin reported a resistance factor of 33 to this compound which is similar to the level of resistance displayed by other aphid clones with the R3 level of esterase overproduction [13]. However, the enhanced level of carboxylesterase is unlikely to confer significant resistance to neonicotinoids since other M. persicae clones overproducing this enzyme show no resistance to imidacloprid [22].…”

Section: Discussionmentioning

confidence: 60%

“…However the level of expression between these sequences was variable (ranging from 13–63 fold). This variation probably resulted from the fact that only one of the five ESTs (contig 3118) was a perfect match with FE4 at the probe site, shown previously to be the variant present in 5191A [13]. Therefore, the fold-change indicated by the probe designed on contig 3118 is likely to be the most accurate and this level corresponds well with the 64-fold increase in the esterase level previously reported in aphids with the FE4 genes and R3 levels of resistance [19].…”

Section: Resultsmentioning

confidence: 98%

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Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae

et al. 2010

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The aphid Myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine receptors (nAChRs), have remained an effective control measure; however, recent reports of resistance in M. persicae represent a threat to the long-term efficacy of this chemical class. In this study, the mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches. Bioassays on a resistant M. persicae clone (5191A) suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute. Microarray analysis, using an array populated with probes corresponding to all known detoxification genes in M. persicae, revealed constitutive over-expression (22-fold) of a single P450 gene (CYP6CY3); and quantitative PCR showed that the over-expression is due, at least in part, to gene amplification. This is the first report of a P450 gene amplification event associated with insecticide resistance in an agriculturally important insect pest. The microarray analysis also showed over-expression of several gene sequences that encode cuticular proteins (2–16-fold), and artificial feeding assays and in vivo penetration assays using radiolabeled insecticide provided direct evidence of a role for reduced cuticular penetration in neonicotinoid resistance. Conversely, receptor radioligand binding studies and nucleotide sequencing of nAChR subunit genes suggest that target-site changes are unlikely to contribute to resistance to neonicotinoid insecticides in M. persicae.

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Section: Discussionmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 98%

Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae

et al. 2010

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“…These evidences indicate that the single point mutation (R81T) obtained in this study should be responsible for the imidaclopridresistance in the field population of A. gossypii. P450-mediated detoxification and target site insensitivity have been confirmed to affect the development of imidacloprid resistance in M. persicae [8,9,23]. However, Kool et al reported that the P450, GST and EST detoxification enzymes have no significant differences between the S and IMI-R strains of A. gossypii, and only the nAChRβ1 R81T point mutation has an effect on the imidacloprid resistance of A. gossypii [4].…”

Section: Discussionmentioning

confidence: 94%

Frequency detection of imidacloprid resistance allele in Aphis gossypii field populations by real-time PCR amplification of specific-allele (rtPASA)

Zhang

Cui

et al. 2015

Pesticide Biochemistry and Physiology

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“…Some xenobiotics can modulate the activity of not only detoxification enzymes in living organisms, but also of digestive and energy metabolism enzymes (Nath et al ., ; Nath, ; Natsuhara et al ., ; Philippou & Moores, ). Enhanced detoxification activity is frequently associated with insecticide resistance, but the activity of digestive and energy metabolism enzymes may also be important in mitigating fitness costs usually associated with insecticide resistance (Araújo et al ., ,b; Scott, ; Hemingway, ; Silva et al ., ,b).…”

Section: Introductionmentioning

confidence: 99%

Does cypermethrin affect enzyme activity, respiration rate and walking behavior of the maize weevil (Sitophilus zeamais)?

Veloso¹,

Pereira²,

Guedes³

et al. 2012

Insect Science

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Insecticides cause a range of sub-lethal effects on targeted insects, which are frequently detrimental to them. However, targeted insects are able to cope with insecticides within sub-lethal ranges, which vary with their susceptibility. Here we assessed the response of three strains of the maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) to sub-lethal exposure to the pyrethoid insecticide cypermethrin. We expected enzyme induction associated with cypermethrin resistance since it would aid the resistant insects in surviving such exposure. Lower respiration rate and lower activity were also expected in insecticide-resistant insects since these traits are also likely to favor survivorship under insecticide exposure. Curiously though, cypermethrin did not affect activity of digestive and energy metabolism enzymes, and even reduced the activity of some enzymes (particularly for cellulase and cysteine-proteinase activity in this case). There was strain variation in response, which may be (partially) related to insecticide resistance in some strains. Sub-lethal exposure to cypermethrin depressed proteolytic and mainly cellulolytic activity in the exposed insects, which is likely to impair their fitness. However, such exposure did not affect respiration rate and walking behavior of the insects (except for the susceptible strain where walking activity was reduced). Walking activity varies with strain and may minimize insecticide exposure, which should be a concern, particularly if associated with (physiological) insecticide resistance.

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Metabolic enzyme(s) confer imidacloprid resistance in a clone of Myzus persicae (Sulzer) (Hemiptera: Aphididae) from Greece

Abstract: A combination of in vitro biochemical assays and in vivo differential synergism studies using PBO and a close analogue EN 16/5-1 suggests that the mechanism conferring increased resistance in this clone is primarily due to enhanced oxidase activity.

Cited by 71 publications

References 28 publications

Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae

Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae

Frequency detection of imidacloprid resistance allele in Aphis gossypii field populations by real-time PCR amplification of specific-allele (rtPASA)

Does cypermethrin affect enzyme activity, respiration rate and walking behavior of the maize weevil (Sitophilus zeamais)?

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