Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis

Tseng, Chung‐Chih; Lin, Yu Ju; Liu, Wangta; Lin, Hao; Chou, Hsin-Yu; Thia, Caroline; Wu, Jou Hsien; Chang, Jo Shu; Wen, Zhi Hong; Chang, Jui Jen; Wang, Hui‐Min David

doi:10.1016/j.fct.2019.110993

Cited by 33 publications

(31 citation statements)

References 48 publications

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“…The cell lines were characterized by Genetic Testing Biotechnology Corporation (Suzhou, China) using short tandem repeat markers and they were not contaminated by mycoplasma detected by Myco-Lumi Luminescent Mycoplasma Detection Kit (Beyotime, Shanghai, China). SFE was separated and purified from radish seeds (Beijing Tongrentang Co., LTD, Beijing, China) as reported previously 2,3 , dissolved in DMSO (Beijing Chemical Factory, Beijing, China). SB202190 was obtained from MedChem Express (Monmouth Junction, NJ, USA).…”

Section: Cell Culture and Chemicalsmentioning

confidence: 99%

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Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop

Han

Wang

et al. 2020

Cell Death Dis

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Esophageal cancer is one of the most common cancer with limited therapeutic strategies, thus it is important to develop more effective strategies to against it. Sulforaphene (SFE), an isothiocyanate isolated from radish seeds, was proved to inhibit esophageal cancer progression in the current study. Flow cytometric analysis showed SFE induced cell apoptosis and cycle arrest in G2/M phase. Also, scrape motility and transwell assays presented SFE reduced esophageal cancer cell metastasis. Microarray results showed the influence of SFE on esophageal cancer cells was related with stearoyl-CoA desaturase (SCD), cadherin 3 (CDH3), mitogen-activated protein kinase kinase 3 (MAP2K3) and growth arrest and DNA damage inducible beta (GADD45B). SCD and CDH3 could promote esophageal cancer metastasis via activating the Wnt pathway, while the latter one was involved in a positive feedback loop, GADD45B-MAP2K3-p38-p53, to suppress esophageal cancer growth. GADD45B was known to be the target gene of p53, and we proved in this study, it could increase the phosphorylation level of MAP2K3 in esophageal cancer cells, activating p38 and p53 in turn. SFE treatment elevated MAP2K3 and GADD45B expression and further stimulated this feedback loop to better exert antitumor effect. In summary, these results demonstrated that SFE had the potential for developing as a chemotherapeutic agent because of its inhibitory effects on esophageal cancer metastasis and proliferation.

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Section: Cell Culture and Chemicalsmentioning

confidence: 99%

“…With the development of research on cancer prevention and treatment, accumulated epidemiological evidence indicated that many natural active substances, such as astaxanthin 2 and resveratrol 3 had inhibitory effects on multiple cancers. Among them, isothiocyanates from cruciferous vegetables have attracted more and more attention.…”

Section: Introductionmentioning

confidence: 99%

Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop

Han

Wang

et al. 2020

Cell Death Dis

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“…Based on our previous findings, we discovered AST suppressed melanoma proliferation via apoptotic mechanism in vitro and in a xenograft model [6,7], and the lung metastatic melanoma inhibition by the oral TAP-nanoemulsion administration within this work was demonstrated. Bcl-2 family proteins are well known as major regulators of the mitochondrial apoptosis mechanism.…”

Section: Tap-nanoemulsion Inhibits Lung Metastatic Melanomamentioning

confidence: 62%

“…To determine whether TAP-nanoemulsion suppresses oxidations, we investigate ROS generations in cell-based foreskin fibroblasts. PMA is an inducer for endogenous superoxide secretions, and PMA-activated cells produced 6 Oxidative Medicine and Cellular Longevity ROS which emitted the fluorescent intensity to be detected [23]. In Figure 3, it was demonstrated that TAPnanoemulsion gradually reduced oxidative stresses in a dose-dependent manner.…”

Section: Antioxidative Assay Of Tap-nanoemulsionmentioning

confidence: 93%

“…During the digestion process, TAP-nanoemulsion passed through three phases, including the oral, gastric, and small intestine stages. It should be noted that the size and interface characteristics of nanoemulsion might be changed during component assimilations [5,6]. Our sample was in an aqueous gavage form (hence, the chewing procedure was irrelevant), and the oral residence time was ignored.…”

Section: Tap-nanoemulsion Bioaccessibility Measurement Inmentioning

confidence: 99%

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A Novel Oral Astaxanthin Nanoemulsion from Haematococcus pluvialis Induces Apoptosis in Lung Metastatic Melanoma

Haung

Wang

Cheng

et al. 2020

Oxidative Medicine and Cellular Longevity

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Astaxanthin (AST) is a naturally occurring xanthophyll carotenoid having the potential to be used as an anticancer agent; however, the human body has a low bioavailability of AST due to its poor solubility in the water phase. Therefore, we applied D-α-tocopheryl polyethylene glycol succinate (TPGS) as an emulsifier and natural edible peanut oil to form a steady oil-in-water (O/W) nanoemulsion loaded with AST (denoted as TAP-nanoemulsion). TAP-nanoemulsions were stable without the droplet coalescence against thermal treatments (30-90°C), pH value changes (over a range of 2.0-8.0), and ionic strength adjustments (at NaCl concentrations of 100-500 mM) measured by dynamic light scattering (DLS). AST within TAP-nanoemulsion was released up to 80% in a simulated intestinal enzymatic fluid in vitro, and the overall recovery rate was fairly consistent in the Caco-2 cellular model. In order to further evaluate in vivo melanoma inhibitory experiments, we injected the fluorescent-stained B16F10 cells into female C57BL/6 mouse tail veins and treated TAP-nanoemulsion in an oral gavage. qRT-PCR and Western blot demonstrated that TAP-nanoemulsion triggered effectively the apoptosis pathway, including enhancements of cleaved caspase-3 and caspase-9, ataxia-telangiectasia mutated kinase (ATM), and p21WAF1/CIP1 (p21) and decreases of B-cell lymphoma 2 (Bcl-2); cyclins D, D1, and E; mitogen-activated protein kinase (MEK); extracellular signal-regulated kinases (ERK); nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB); and matrix metallopeptidase-1 and metallopeptidase-9 (MMP-1 and MMP-9) in both gene and protein expressions. In conclusion, this study suggests that TAP-nanoemulsion with the oral treatment has a positive chemotherapy effect in melanoma with lung metastases in vivo. As far as we know, this is the first time to demonstrate that an antioxidant in nanoparticle administration cures lung metastatic melanoma.

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Probiotic Yeasts

Shahryari

Sadeghi²

2023

Handbook of Food Bioactive Ingredients

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Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis

Cited by 33 publications

References 48 publications

Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop

Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop

A Novel Oral Astaxanthin Nanoemulsion from Haematococcus pluvialis Induces Apoptosis in Lung Metastatic Melanoma

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