Metabolic engineering of Zymomonas moblis for ethylene production from straw hydrolysate

Metabolic engineering ofZymomonas mobilisfor continuous co-production of bioethanol and poly-3-hydroxybutyrate (PHB)

“…mobilis has been developing as a chassis to construct microbial cell factories for biochemical production such as lignocellulosic ethanol, 2,3-butanediol, isobutanol, lactate, and ethylene. 25,31,32,60,61 In this study, recombinant strains of Z. mobilis were constructed for PHB production using genetic and process engineering approaches as well as fermentation condition optimization and their combinations (Fig. 1 and 7).…”

“…Moreover, exogenous and native CRISPR-Cas genome-editing toolkits and biological part identification and characterization systems have been established in Z. mobilis, [24][25][26][27][28][29] and various heterologous metabolic pathways have been constructed to produce products such as lactate, 2,3-butanediol (2,3-BDO), isobutanol, and ethylene. 20,[30][31][32] The heterologous PHB pathway has also been engineered into Z. mobilis driven by a strong native promoter Ppdc with 0.7% (DCW) PHB being produced. 33 Different metabolic engineering strategies were applied and combined to increase the heterologous PHB production in Z. mobilis in this study, including overexpression of the phaCAB operon by using a strong promoter and/or increasing the copy number, redox balance through cofactor-system modification, and competition of acetaldehyde for acetyl-CoA generation through the ethanol utilization pathway (EUP).…”

Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis

“…The generating genome-editing plasmid, named pMini-TSacB (150 ng), was introduced into Z. mobilis S912 competent cells using a Gene Pulse Xcell (0.1 cm gap cuvettes, 1.6 kV, 200 Ω, 25 μF) (Bio-Rad). Transformants were selected at 30°C in RM supplemented with 100 mg/L spe (He et al, 2021). Microbial PCR was used to screen mutant candidates using primers sacB 0374 KO0374-F and sacB 0374 KO0374-R (Table 2).…”

One Advantage of Being Polyploid: Prokaryotes of Various Phylogenetic Groups Can Grow in the Absence of an Environmental Phosphate Source at the Expense of Their High Genome Copy Numbers

“…It can convert simple sugars into ethanol at nearly the maximal theoretical yield, with a higher efficiency than Sacchomyces cerevisiae [ 27 , 28 ]. However, in addition to Z. mobilis being applied to produce ethanol, its metabolism is also designed for the production of a wide range of different products [ 29 , 30 , 31 , 32 ]. A recent study revealed that Z. mobilis is polyploid with about 20 genome copies throughout growth [ 33 ].…”