Metabolic Engineering of Wheat Provitamin A by Simultaneously Overexpressing <i>CrtB</i> and Silencing Carotenoid Hydroxylase (<i>TaHYD</i>)

Zeng, Jian; Wang, Xiatian; Miao, Yingjie; Wang, Cheng; Zang, Mingli; Chen, Xi; Li, Miao; Li, Xiaoyan; Wang, Qiong; Li, Kexiu; Chang, Junli; Wang, Yue-Sheng; Yang, Guangxiao; He, Guangyuan

doi:10.1021/acs.jafc.5b04279

Cited by 62 publications

(38 citation statements)

References 56 publications

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“…10-fold enrichment [293,294]. Overexpression of crtB together with endospermspecific silencing of an endogenous BCH gene (TaHYD) was also successful to increase β-carotene content to 2.45 μg/g DW in wheat endosperm [295].…”

Section: Other Cerealsmentioning

confidence: 95%

A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health

Rodríguez‐Concepción

Ávalos

Bonet

et al. 2018

Progress in Lipid Research

718

584

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Carotenoids are lipophilic isoprenoid compounds synthesized by all photosynthetic organisms and some non-photosynthetic prokaryotes and fungi. With some notable exceptions, animals (including humans) do not produce carotenoids de novo but take them in their diets. In photosynthetic systems carotenoids are essential for photoprotection against excess light and contribute to light harvesting, but perhaps they are best known for their properties as natural pigments in the yellow to red range. Carotenoids can be associated to fatty acids, sugars, proteins, or other compounds that can change their physical and chemical properties and influence their biological roles. Furthermore, oxidative cleavage of carotenoids produces smaller molecules such as apocarotenoids, some of which are important pigments and volatile (aroma) compounds. Enzymatic breakage of carotenoids can also produce biologically active molecules in both plants (hormones, retrograde signals) and animals (retinoids). Both carotenoids and their enzymatic cleavage products are associated with other processes positively impacting human health. Carotenoids are widely used in the industry as food ingredients, feed additives, and supplements. This review, contributed by scientists of complementary disciplines related to carotenoid research, covers recent advances and provides a perspective on future directions on the subjects of carotenoid metabolism, biotechnology, and nutritional and health benefits.

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Section: Other Cerealsmentioning

confidence: 95%

A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health

Rodríguez‐Concepción

Ávalos

Bonet

et al. 2018

Progress in Lipid Research

718

584

View full text Add to dashboard Cite

show abstract

“…By simultaneously introducing the PaCRTB and the PaCRTI genes into a low carotenoid bread wheat varieties, the same authors achieved 8-fold increase in the total carotenoid content up to 4.76 µg/g and 76-fold increase in the provitamin A carotenoid content that reached 3.82 µg/g, most of which was represented by β-carotene (3.21 µg/g) [38] (Table 1). Further increase in the β-carotene content was obtained by the same research group by combining the endosperm-specific silencing of the HYD gene and the overexpression of the bacterial CRTB gene [39]. Through this combination of methods, significant levels of β-carotene accumulation were obtained, corresponding to an increase of up to 31-fold and a concentration of up to 5.04 µg/g (Table 1).…”

Section: Compositionmentioning

confidence: 95%

Carotenoids in Cereal Food Crops: Composition and Retention throughout Grain Storage and Food Processing

Trono

2019

Plants

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Carotenoids are C40 isoprenoids synthesized by plants, as well as some bacteria, fungi and algae, that have been reported to be responsible for a number of benefits conferred on human health. The inability of animals and humans to synthesize de novo these compounds is the reason why they must be introduced from dietary sources. In cereal grains, carotenoids are important phytochemicals responsible for the characteristic yellow colour of the endosperm, which confers nutritional and aesthetic quality to cereal-based products. Cereals are staple foods for a large portion of the world population, and the biofortification of cereal grains with carotenoids may represent a simple way to prevent many human diseases and disorders. Unfortunately, evidence exists that the storage and processing of cereal grains into food products may negatively impact their carotenoid content; so, this loss should be taken into consideration when analysing the potential health benefits of the cereal-based products. Focusing on the recent updates, this review summarizes the chemical composition of the carotenoids in the grains of staple cereals, including wheat, maize, rice and sorghum, the main factors that affect their carotenoid content during storage and processing and the most fruitful strategies used improve the grain carotenoid content and limit the carotenoid post-harvest losses.

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“…To achieve a high level of β-carotene accumulation, suppressing the activity of LYCE by silencing StLCY-e in potato or the activity of HYDB by silencing StCHY-𝜀 in potato is adopted to ensure the appropriate direction of the metabolic flux (Diretto et al, 2007; Van Eck et al, 2007). In wheat, simultaneous overexpression of CrtB and silencing of HYDB achieves an up to 31-fold increase of β-carotene up to 5.06 μg g -1 (Zeng et al, 2015). Another approach is to make use of the Orange ( Or ) gene from cauliflower ( Brassica oleracea var.…”

Section: Manipulation Of Vitamin Metabolismmentioning

confidence: 99%

Manipulation of Metabolic Pathways to Develop Vitamin-Enriched Crops for Human Health

et al. 2017

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Vitamin deficiencies are major forms of micronutrient deficiencies, and are associated with huge economic losses as well as severe physical and intellectual damages to humans. Much evidence has demonstrated that biofortification plays an important role in combating vitamin deficiencies due to its economical and effective delivery of nutrients to populations in need. Biofortification enables food plants to be enriched with vitamins through conventional breeding and/or biotechnology. Here, we focus on the progress in the manipulation of the vitamin metabolism, an essential part of biofortification, by the genetic modification or by the marker-assisted selection to understand mechanisms underlying metabolic improvement in food plants. We also propose to integrate new breeding technologies with metabolic pathway modification to facilitate biofortification in food plants and, thereby, to benefit human health.

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Metabolic Engineering of Wheat Provitamin A by Simultaneously Overexpressing CrtB and Silencing Carotenoid Hydroxylase (TaHYD)

Cited by 62 publications

References 56 publications

A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health

A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health

Carotenoids in Cereal Food Crops: Composition and Retention throughout Grain Storage and Food Processing

Manipulation of Metabolic Pathways to Develop Vitamin-Enriched Crops for Human Health

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