Metabolic Engineering of Plant Cells for Biotransformation of Hesperedin into Neohesperidin, a Substrate for Production of the Low-Calorie Sweetener and Flavor Enhancer NHDC

Frydman, Ahuva; Weisshaus, Oori; Huhman, David V.; Sumner, Lloyd W.; Bar‐Peled, Maor; Lewinsohn, Efraim; Fluhr, Robert; Gressel, Jonathan; Eyal, Yoram

doi:10.1021/jf051509m

Cited by 39 publications

(17 citation statements)

References 18 publications

(24 reference statements)

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“…From that point of view, the CitEST Project is a great source of information concerning gene expression patterns of flavedo as a support to gene expression analysis during fruit development and ripening. Moreover, even though the juice is undoubtedly the most important orange product, growing attention has been paid to citrus flavedoderived compounds such as flavonoids (Frydman et al, 2005) and essential oils (Sawamura et al, 2004;Souza et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

In silico prediction of gene expression patterns in Citrus flavedo

et al. 2007

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Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in flavedo, are shown to be promising candidates for further characterization. In addition to promoter seeking, this kind of analysis can lead to gene discovery, tissue-specific and tissue-enriched expression pattern predictions (as shown herein) and can also be adopted as an in silico first, and probably reliable approach, for detecting expression profiles from EST sequencing efforts before experimental validation is available or for heuristically guiding that validation.

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Section: Discussionmentioning

confidence: 99%

In silico prediction of gene expression patterns in Citrus flavedo

et al. 2007

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“…B, an increase in the ethanol/water ratio from 40 to 70% coupled with a decrease in the alkaline amount from 200 mg to 0 mg has positive influence on response variable Y . Mauro et al and Frydman et al have revealed that, with hot water extraction, peel/Ca(OH) 2 g/g of 20 and water/peel g/g of 5 were the best conditions for hesperidin extraction from Chenpi, which was presumably related to the environment pH during the hesperidin crystallization. However, exorbitance amount of alkaline may facilitate the release of pectin against antioxidative compounds, which poses a negative influence on the antioxidants extracted from Chenpi.…”

Section: Resultsmentioning

confidence: 99%

Multi-criteria optimization for ultrasonic-assisted extraction of antioxidants from Pericarpium Citri Reticulatae using response surface methodology, an activity-based approach

et al. 2013

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An activity-based approach to optimize the ultrasonic-assisted extraction of antioxidants from Pericarpium Citri Reticulatae (Chenpi in Chinese) was developed. Response surface optimization based on a quantitative composition-activity relationship model showed the relationships among product chemical composition, antioxidant activity of extract, and parameters of extraction process. Three parameters of ultrasonic-assisted extraction, including the ethanol/water ratio, Chenpi amount, and alkaline amount, were investigated to give optimum extraction conditions for antioxidants of Chenpi: ethanol/water 70:30 v/v, Chenpi amount of 10 g, and alkaline amount of 28 mg. The experimental antioxidant yield under the optimum conditions was found to be 196.5 mg/g Chenpi, and the antioxidant activity was 2023.8 μmol Trolox equivalents/g of the Chenpi powder. The results agreed well with the second-order polynomial regression model. This presented approach promised great application potentials in both food and pharmaceutical industries.

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“…As seen from the previous examples, the polyphenolic compounds are the most important and abundant types of antioxidants in tree bark. Potential polyphenol uses are broad and include the production of natural food preservatives (Seeram -Heber 2007, Coté et al 2011, Gyawali -Ibrahim 2014, Kobus-Cisowska et al 2014, healthcare, and healthcare-related products (Packer et al 1999, Dzialo et al 2016, Watson et al 2018, natural growth bioregulators (Popa et al 2002, Vyvyan 2002, food and beverage products (Frydman et al 2005, Sawalha et al 2009, and silver nanoparticle production (Fahimirida et al 2019, Ranoszek-Soliwoda et al 2019, Rolim et al 2019. A recent global survey predicted a boom in the polyphenol market due to increasing demand and market size.…”

Section: Introductionmentioning

confidence: 99%

Combined Multi-assay Evaluation of the Antioxidant Properties of Tree Bark

Tálos-Nebehaj

Albert

Visi-Rajczi

et al. 2019

Acta Silvatica Et Lignaria Hungarica

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Wood logging generates considerable amounts of bark by-product, which are a potential antioxidant source well worth extracting and using. The present work compares the antioxidant properties of the bark of the following selected Hungarian forest tree species: white poplar (Populus alba L.), black locust (Robinia pseudoacacia L.), sessile oak (Quercus petraea Liebl.), black poplar (Populus nigra L.), silver birch (Betula pendula Roth), European larch (Larix decidua Mill.), scots pine (Pinus sylvestris L.), wild cherry (Prunus avium L.), European hornbeam (Carpinus betulus L.) and sweet chestnut (Castanea sativa Mill.). Inner and outer bark were investigated separately. Total polyphenol content (TPC) was determined by the Folin-Ciocâlteu method, whereas antioxidant capacity was assayed using the ferric reducing ability of plasma (FRAP), 2.2-diphenyl-1-picrylhydrazyl (DPPH), and ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) methods. The overall antioxidant power of the samples was evaluated using a scoring system that combined the FRAP, DPPH, and ABTS assay results. The TPC levels did not always follow the FRAP, DPPH, and ABTS assay values. Differing reaction mechanisms and sample compositions are possible reasons for this. The presented scoring evaluation was suitable for the assessment and comparison of complex antioxidant properties of tree bark samples. According to the scores, inner bark showed higher scores compared to outer bark for most species with the exceptions of black poplar, black locust, white poplar, sweet chestnut, and European larch. The highest overall antioxidant capacities were determined in the inner bark of wild cherry and the outer bark of sweet chestnut. The species with the overall lowest scores were black locust and black poplar.

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Metabolic Engineering of Plant Cells for Biotransformation of Hesperedin into Neohesperidin, a Substrate for Production of the Low-Calorie Sweetener and Flavor Enhancer NHDC

Cited by 39 publications

References 18 publications

In silico prediction of gene expression patterns in Citrus flavedo

In silico prediction of gene expression patterns in Citrus flavedo

Multi-criteria optimization for ultrasonic-assisted extraction of antioxidants from Pericarpium Citri Reticulatae using response surface methodology, an activity-based approach

Combined Multi-assay Evaluation of the Antioxidant Properties of Tree Bark

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