Metabolic engineering of microorganisms: general strategies and drug production

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts

“…Physiological knowledge of the pathways under investigation, choice of the right production organism, information from metabolic flux modelling and bioprocess development need to be considered and addressed in the design of the synthetic multifunctional DNA circuits to be delivered [74]. This can then be exploited for the improved production, up to fermenter scale, of protein therapeutics such as monoclonal antibodies, commodity chemicals such as vitamins or rare amino acids, valuable metabolites, biomolecules eliciting fragrances and flavors, rare natural (medicinal) compounds (such as artemisinin and taxol) or even biofuel production [74][75][76][77][78].…”

“…This can then be exploited for the improved production, up to fermenter scale, of protein therapeutics such as monoclonal antibodies, commodity chemicals such as vitamins or rare amino acids, valuable metabolites, biomolecules eliciting fragrances and flavors, rare natural (medicinal) compounds (such as artemisinin and taxol) or even biofuel production [74][75][76][77][78]. While some constraints can be overcome by optimizing culture conditions, others can more successfully be tackled by modifying defined metabolic pathways.…”

Bench-top fermentative production of plant benzylisoquinoline alkaloids using a bacterial platform

“…[1][2][3][4][5] We previously constructed an (S)-reticuline-producing strain of Escherichia coli, which can produce (S)-reticuline from glucose or glycerol. The platform strain has three pathways: (1) an L-tyrosine over-producing pathway; (2) a dopamineproducing pathway from L-tyrosine; and (3) an (S)-reticuline-producing pathway from dopamine 6 (Fig.…”

Deep Neural Network Method for the Prediction of Xylitol Production

“…On the other hand, E. Coli usually has been used by researchers as a microbial hosts due to their flexible conditions which are easy to culture, grown and manipulate in a laboratory setting. Moreover, E. Coli is a versatile platform organism that always been used for the overproduction of non-native as well as native metabolites [2][3][4][5][6][7][8]. According to Cirino et al [9], by engineers E. Coli, high level of xylitol can be produced from a mixture of xylose and glucose.…”