Metabolic Engineering of Microorganisms for Vitamin C Production

Zhou, Jingwen; Du, Guocheng; Chen, Jian

doi:10.1007/978-94-007-5055-5_12

Cited by 17 publications

(7 citation statements)

References 53 publications

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“…One of the most important disadvantages is the higher manufacturing cost of the fermentation process compared to other similar large block fermentation products. If vitamin C could be directly produced from inexpensive D-glucose by a one-step fermentation process, the final cost of vitamin C would be likely to significantly decreased [ 5 ]. Theoretically, secondary metabolite compounds can be biosynthesized by the metabolic engineering of genes involved in their biosynthesis pathways in a microbial host.…”

Section: Resultsmentioning

confidence: 99%

“…The process has been the main approach in producing vitamin C for over 60 years, while it has inherent disadvantages of a long production time, complex procedures, high temperature and pressure requirements, and the continuous operation difficulties. In addition, the process requires the use of a large number of toxic chemicals that consume high amounts of energy and that may seriously pollute the environment [ 5 ]. Therefore, researchers have tried to find new methods by which to improve the performance of the traditional Reichstein process [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the process requires the use of a large number of toxic chemicals that consume high amounts of energy and that may seriously pollute the environment [ 5 ]. Therefore, researchers have tried to find new methods by which to improve the performance of the traditional Reichstein process [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, novel biotechnological processes that directly convert D-glucose to vitamin C through one-step fermentation would be desirable. Thus, significant effort has been made to develop a one-step vitamin C fermentation process [ 5 ]. However, 2-KLG, not vitamin C, is synthesized in almost all current one-step vitamin C fermentation processes [ 8 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose

Tian

Deng

Zhang

et al. 2022

Biotechnol Biofuels

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Background Production of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-glucose. Therefore, significant effort has been made to develop a one-step vitamin C fermentation process. While, 2-KLG, not vitamin C, is synthesized from nearly all current one-step fermentation processes. Vitamin C is naturally synthesized from glucose in Arabidopsis thaliana via a ten-step reaction pathway that is encoded by ten genes. The main objective of this study was to directly produce vitamin C from D-glucose in Escherichia coli by expression of the genes from the A. thaliana vitamin C biosynthetic pathway. Results Therefore, the ten genes of whole vitamin C synthesis pathway of A. thaliana were chemically synthesized, and an engineered strain harboring these genes was constructed in this study. The direct production of vitamin C from D-glucose based on one-step fermentation was achieved using this engineered strain and at least 1.53 mg/L vitamin C was produced in shaking flasks. Conclusions The study demonstrates the feasibility of one-step fermentation for the production of vitamin C from D-glucose. Importantly, the one-step process has significant advantages compared with the currently used fermentation process: it can save multiple physical and chemical steps needed to convert D-glucose to D-sorbitol; it also does not involve the associated down-streaming steps required to convert 2-KLG into vitamin C.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose

Tian

Deng

Zhang

et al. 2022

Biotechnol Biofuels

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“…However, the enzyme activity of the SDH is very low and can only produce several grams of 2-KLG by different optimization method. Based the previous reports about other SDHs that can convert L-sorbose to Lsorbosone or 2-KLG (Zhou et al, 2012;Gao et al, 2017), both substrate/product specificity and enzyme activity should be improved by high-throughput screening. Since the slow growth rate of G. oxydans and low transformation efficiency (Yao et al, 2017;Jin et al, 2019), G. oxydans itself is not suitable for highthroughput screening of efficient SDH.…”

Section: Introductionmentioning

confidence: 99%

High Throughput Screening Platform for a FAD-Dependent L-Sorbose Dehydrogenase

Shan

Liu

Zeng

et al. 2020

Front. Bioeng. Biotechnol.

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2-Keto-L-gulonic acid (2-KLG) is the direct precursor for the production of L-ascorbic acid (L-Asc) on industrial scale. Currently, the production of L-Asc in the industry is a two-step fermentation process. Owing to many unstable factors in the fermentation process, the conversion rate of L-sorbose to 2-KLG has remained at about 90% for many years. In order to further improve the production efficiency of 2-KLG, a FAD-dependent sorbose dehydrogenase (SDH) has been obtained in our previous research. The SDH can directly convert L-sorbose to 2-KLG at a very high efficiency. However, the enzyme activity of the SDH is relatively low. In order to further improve the enzyme activity of the SDH, a high throughput screening platform the dehydrogenase is essential. By optimizing the promoter, host and sorbosone dehydrogenase (SNDH), knockout of the aldosterone reductases and PTS related genes, a reliable platform for high-throughput screening of more efficient FAD-dependent SDH has been established. By using the high-throughput screening platform, the titer of the 2-KLG has been improved by 14.1%. The method established here could be useful for further enhancing the FAD-dependent SDH, which is important to achieve the efficient one-strain-single-step fermentation production of 2-KLG.

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Sugar Alcohols

Cock

2020

Kirk-Othmer Encyclopedia of Chemical Technology

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Sugar alcohols, or polyols, are carbohydrates. They bear a close relationship to the saccharides from which they are formed by reduction and from which their names are often derived; glucitol (sorbitol), mannitol, isomalt, maltitol, lactitol, xylitol, and erythritol are the most common commercially available polyols. Some occur in nature in small amounts. They are generally produced by hydrogenation or fermentation of saccharides from renewable raw materials. Worldwide consumption of polyols is estimated to be about 1.8 million metric tons in 2020. More than half of that volume is used in foods where polyols are used at high levels as sugar‐free bulk sweeteners (sugar content less than 0.5% based on dry solids) replacing sugars on a weight‐for‐weight basis and at lower levels to perform very specific technical functionalities. Also, more than half of the global polyols consumption relates to sorbitol only, of which about three‐quarters is used in nonfood applications such as in oral and personal care (ie, toothpaste) and as a starting material in the production of, for example, vitamin C, polymers, and surfactants. Polyols are used in pharmaceutical and cosmetic applications mainly for technical functionality such as filler, binder, anticaking agent, stabilizer, texturizer, moisturizer, and humectant. This article provides an overview of the most common polyols; how they are produced; their physical, chemical, sensorial, biological, and nutritional characteristics; properties in food and nonfood applications; and legal aspects associated with their use.

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Metabolic Engineering of Microorganisms for Vitamin C Production

Cited by 17 publications

References 53 publications

Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose

Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose

High Throughput Screening Platform for a FAD-Dependent L-Sorbose Dehydrogenase

Sugar Alcohols

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