Metabolic engineering of Klebsiella pneumoniae J2B for the production of 1,3-propanediol from glucose

Lama, Suman; Seol, Eunhee; Park, Sunghoon

doi:10.1016/j.biortech.2017.05.052

Cited by 28 publications

(19 citation statements)

References 29 publications

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“…Genes related to acetate production and metabolism were searched based on previous studies (Lama et al 2017;Garsin 2010) and using the KEGG database (Fig. 3) (Kanehisa and Goto 2000).…”

Section: Genes and Pathways Involved In Acetate Productionmentioning

confidence: 99%

Complete genome sequence of acetate-producing Klebsiella pneumoniae L5-2 isolated from infant feces

et al. 2019

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Acetate is an important metabolite in infants as it can affect metabolism as well as immune and inflammatory responses. However, there have been no studies on acetate production by Klebsiella pneumoniae isolated from infant feces. In this study, we isolated a K. pneumoniae strain, L5-2, from infant feces, and we found it produces acetate. The genome of L5-2 consisted of a 5,237,123-bp single chromosome and a 139,211-bp single plasmid. The G + C content was 57.27%. By whole-genome analysis of K. pneumoniae L5-2, we identified seven genes related to acetate production (poxA, pta, eutD, ackA, eutP, eutQ, and adhE). We confirmed acetate production by K. pneumoniae L5-2 by ion chromatography. The aldehyde/alcohol dehydrogenase (adhE) activity of K. pneumoniae L5-2 was significantly higher than that of the K. pneumoniae subsp. ozaenae ATCC 11296. Thus, the acetate-producing ability of K. pneumoniae L5-2 was influenced by the adhE gene. In addition, K. pneumoniae L5-2 had significantly less virulence factor-encoding genes than other K. pneumoniae strains isolated from humans. In conclusion, K. pneumoniae L5-2 isolated from infant feces has less virulence factors and higher adhE activity than other K. pneumoniae strains.

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“…Genes related to acetate production and metabolism were searched based on previous studies (Lama et al 2017;Garsin 2010) and using the KEGG database (Fig. 3) (Kanehisa and Goto 2000).…”

Section: Genes and Pathways Involved In Acetate Productionmentioning

confidence: 99%

Complete genome sequence of acetate-producing Klebsiella pneumoniae L5-2 isolated from infant feces

et al. 2019

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“…The glycerol is converted into 1,3-PD by a two-step reductive pathway, including glycerol dehydratase (DhaB) catalyzed 3hydroxypropionaldehyde (3-HPA) synthesis from glycerol and a following reduced nicotinamide adenine dinucleotide (NADH)-dependent 1,3-PD oxidoreductase (DhaT) mediated 1,3-PD formation [6]. The conversion rate of glycerol to 1,3-PD was increased by strain overexpressing DhaB and DhaT [7]. Furthermore, the NAD(+)-dependent gammaglutamyl-gamma-aminobutyraldehyde dehydrogenase (PuuC) was demonstrated to be another hypothetical oxidoreductase for 1,3-PD formation [8].…”

Section: Introductionmentioning

confidence: 99%

Klebsiella Pneumoniae Metabolic Regulation and its Application in 1,3-Propanediol Production

Wang¹,

Mu²,

Liu³

et al. 2021

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Klebsiella pneumoniae is a well-known model organism for glycerol metabolism to produce 1,3-propanediol (1,3-PD), a valuable chemical intermediate for materials, such as polyesters. However, the relatively low conversion rate and productivity, as well as the accumulation of by-products such as lactic acid, ethanol and acetic acid, inhibit the production of 1,3-PD. Hereby, the 1,3-PD metabolism in K. pneumoniae was regulated through pathway engineering by using CRISPR-Cas9 technology for the first time to knock out the ldhA gene of lactate dehydrogenase,the adhE gene of alcohol dehydrogenase and the ack gene of acetate kinase respectively as needed and constructed recombinant bacteria ldhA(−), ldhA(−)-ack(−), ldhA(−)-adhE(−) and ldhA(−)-adhE(−)-ack(−), all of which showed a decrease in by-product production, leading to a higher NADH availability, and 1,3-BD production was significantly increased. In the shake flask fermentation, the 1,3-PD yield and conversion rate of the recombinant strain ldhA(−), ldhA(−)-ack(−), ldhA(−)-adhE(−), ldhA(−)-adhE(−)-ack(−) were higher than those of the parent strain. In the fed-batch fermentation, the 1,3-PD yield and conversion rate of the recombinant strain ldhA(−) were higher than those of the parent strain. The biomass of the recombinant strain ldhA(−)-adhE(−)-ack(−) was reduced due to the accumulation of acetic acid, but its 1,3-PD conversion rate was still higher than that of the parent strain. The higher productivity and fewer by-products concluded that the four Klebsiella pneumoniae recombinant strains could be promising industrial strain for economical production of 1,3-PD.

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“…The bioproduction of 1,3-PDO from glycerol hasb een previously examined. [14,15] Biological glycerolc onversion to 1,3-PDO consists of two consecutive reactions:g lycerolt o3-hydroxypropionaldehyde (3-HPA) by glycerold ehydratase with coenzyme B 12 as ac ofactor,a nd 3-HPAt o1 ,3-PDO by 1,3-propanediol oxidoreductase with ar educing equivalent in the form of NADH (nicotinamide adenine dinucleotide hydrogen) (NADH); [16] two moles of additional reducinge quivalents are necessaryf or glycerol-based 1,3-PDO production [Eqs. (1)…”

Section: Introductionmentioning

confidence: 99%

Small Current but Highly Productive Synthesis of 1,3‐Propanediol from Glycerol by an Electrode‐Driven Metabolic Shift in Klebsiella pneumoniae L17

et al. 2020

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Electrofermentation actively regulates the bacterial redox state, which is essential for bioconversion and has been highlighted as an effective method for further improvements of the productivity of either reduced or oxidized platform chemicals. 1,3‐Propanediol (1,3‐PDO) is an industrial value‐added chemical that can be produced from glycerol fermentation. The bioconversion of 1,3‐PDO from glycerol requires additional reducing energy under anoxic conditions. The cathode‐based conversion of glycerol to 1,3‐PDO with various electron shuttles (2‐hydroxy‐1,4‐naphthoquinone, neutral red, and hydroquinone) using Klebsiella pneumoniae L17 was investigated. The externally poised potential of −0.9 V vs. Ag/AgCl to the cathode increased 1,3‐PDO (35.5±3.1 mm) production if 100 μm neutral red was used compared with non‐bioelectrochemical system fermentation (23.7±2.4 mm). Stoichiometric metabolic flux and transcriptional analysis indicated a shift in the carbon flux toward the glycerol reductive pathway. The homologous overexpression of glycerol dehydratase (DhaB) and 1,3‐PDO oxidoreductase (DhaT) enzymes synergistically enhanced 1,3‐PDO conversion (39.3±0.8 mm) under cathode‐driven fermentation. Interestingly, a small current uptake (0.23 mmol of electrons) caused significant metabolic flux changes with a concomitant increase in 1,3‐PDO production. This suggests that both an increase in 1,3‐PDO production and regulation of the cellular metabolic pathway are feasible by electrode‐driven control in cathodic electrofermentation.

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Metabolic engineering of Klebsiella pneumoniae J2B for the production of 1,3-propanediol from glucose

Cited by 28 publications

References 29 publications

Complete genome sequence of acetate-producing Klebsiella pneumoniae L5-2 isolated from infant feces

Complete genome sequence of acetate-producing Klebsiella pneumoniae L5-2 isolated from infant feces

Klebsiella Pneumoniae Metabolic Regulation and its Application in 1,3-Propanediol Production

Small Current but Highly Productive Synthesis of 1,3‐Propanediol from Glycerol by an Electrode‐Driven Metabolic Shift in Klebsiella pneumoniae L17

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