Metabolic Engineering of<i>Pseudomonas</i>

Nikel, Pablo I.; Lorenzo, Vı́ctor de

doi:10.1002/9783527823468.ch14

Cited by 4 publications

(2 citation statements)

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“…Some species are free-living, some are plant growth-promoting (N2-fixing symbiotic bacteria), while others are considered pathogens or opportunistic pathogens. Thus, many members of this genus are of interest for biotechnological research due to their distribution, the large number of described and cultivated species, and their adaptations to a wide variety of environmental conditions [47][48][49][50]. Among the species of Pseudomonas with some capability of taking up metals (metalloids) are P. alcaliphila, P. aeruginosa, P. fluorescens, P. koreensis, P. mendocina and P. stutzeri (see [50]).…”

Section: Discussionmentioning

confidence: 99%

Bioprospection of Bacterial Strains from Chromite Process Industry Residues from Mexico for Potential Remediation

Martínez-Aldape,

Sandoval-Vergara,

Padilla-Hernández

et al. 2024

Applied Microbiology

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Industrial residues with high concentrations of hexavalent chromium [Cr(VI)], characterized by an alkaline pH (between 9 and 13) and high salinity (around 100 psu), were used as a source for extremophilic chromium-resistant and -reducing microorganisms. An investigation of biodiversity through MiSeq showed the presence of 20 bacterial classes, with Bacilli (47%), Negativicutes (15%), Bacteriodia (8%), Gammaproteobacteria (7%) and Clostridia (5%) being the most abundant. The bioprospection allowed the cultivation of 87 heterotrophic bacterial colonies and 17 bacterial isolates at the end of the isolation, and screening procedures were obtained. The isolates were related to Cellulosimicrobium aquatile, C. funkei, Acinetobacter radioresistens, Staphylococcus equorum, S. epidermis, Brachybacterium paraconglometratum, Glutamicibacter creatinolyticus, Pseudomonas songnenensis, Microbacterium algeriense and Pantoea eucalypti, most of them being resistant to Cr(VI). Resistances of up to 400 mg.L−1 of chromate were obtained for four related strains (QReMLB55A, QRePRA55, QReMLB33A and QReMLB44C). The C. aquatile strain QReMLB55A and the P. songnenensis strain QReMLB33A were exposed to K2Cr2O7 (200 mg.L−1) under optimal conditions, diminishing 94% and 24% of the Cr(VI) in 6 days, respectively. These strains exhibited a high potential for chromium remediation biotechnologies.

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Section: Discussionmentioning

confidence: 99%

Bioprospection of Bacterial Strains from Chromite Process Industry Residues from Mexico for Potential Remediation

Martínez-Aldape,

Sandoval-Vergara,

Padilla-Hernández

et al. 2024

Applied Microbiology

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“…Pseudomonads represent valuable hosts for cell factories due to their versatile metabolism, stress- and solvent tolerance ( Bitzenhofer et al, 2021 ; Schwanemann et al, 2020 ). Moreover, they can be readily engineered by contemporary molecular biology methods ( Nikel and de Lorenzo, 2021 ). Pseudomonas taiwanensis VLB120 is a promising chassis organism with high solvent tolerance and has been efficiently engineered, for example, for styrene epoxidation ( Park et al, 2007 ; Volmer et al, 2017 ), the synthesis of adipic acid and 6-hydroxy-hexanoic acid from cyclohexane ( Bretschneider et al, 2022 ; Schäfer et al, 2020 ) or the synthesis of 4-hydroxybenzoate ( Lenzen et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%