Metabolic engineering of Escherichia coli for the production of four-, five- and six-carbon lactams

Chae, Tong Un; Ko, Yoo-Sung; Hwang, Kyu-Sang; Lee, Sang Yup

doi:10.1016/j.ymben.2017.04.001

Cited by 79 publications

(88 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For direct production of butyrolactam using recombinant E. coli , mutated glutamate decarboxylase ( gadB E89QΔ452–466 ) and β‐alanine CoA transferase ( act ) from Clostridium propionicum were used for the production of GABA and GABA‐CoA. After spontaneous cyclization of GABA‐CoA, 54.1 g L −1 butyrolactam was produced . The synthesis of nylon‐4 was recently demonstrated by the polymerization of GABA from the whole cell conversion of glutamate monosodium salt (MSG) by recombinant E. coli expressing glutamate decarboxylase ( gadB ) from Lactococcus lactis .…”

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

“…5‐Aminovaleric acid (5‐AVA) is used for the production of Δ‐valerolactam, a precursor for the synthesis of bio‐nylons, e.g., nylon‐5 and nylon‐6,5 . Direct production of Δ‐valerolactam and ε‐caprolactam was demonstrated using recombinant E. coli strains expressing lysine 2‐monooxygenase ( davB ) and 5‐aminovaleramidase ( davA ) for 5‐AVA production and either β‐alanine CoA transferase ( act ) from C. propionicum or acyl‐CoA ligase (ORF26) from Streptomyces aizunensis . In C. glutamicum , 5‐AVA can be synthesized from l ‐lysine by two consecutive enzymatic conversions.…”

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

“…Escherichia coli are often used as a model prokaryote for genomic and physiological studies (Archer et al, 2011;Arifin et al, 2014;Ishii et al, 2007;Vijayendran et al, 2007;Yoon et al, 2012), and as host strains for industrial bioprocesses (Chae et al, 2017;S. S.Y.…”

Section: Introductionmentioning

confidence: 99%

RapidRIP quantifies the intracellular metabolome of 7 industrial strains of E. coli

McCloskey

Schrübbers

et al. 2018

Metabolic Engineering

View full text Add to dashboard Cite

A B S T R A C TFast metabolite quantification methods are required for high throughput screening of microbial strains obtained by combinatorial or evolutionary engineering approaches. In this study, a rapid RIP-LC-MS/MS (RapidRIP) method for high-throughput quantitative metabolomics was developed and validated that was capable of quantifying 102 metabolites from central, amino acid, energy, nucleotide, and cofactor metabolism in less than 5 minutes. The method was shown to have comparable sensitivity and resolving capability as compared to a full length RIP-LC-MS/MS method (FullRIP). The RapidRIP method was used to quantify the metabolome of seven industrial strains of E. coli revealing significant differences in glycolytic, pentose phosphate, TCA cycle, amino acid, and energy and cofactor metabolites were found. These differences translated to statistically and biologically significant differences in thermodynamics of biochemical reactions between strains that could have implications when choosing a host for bioprocessing.

show abstract

“…Current published routes to caprolactam production in vivo rely on the cyclization of 6ACA via the activity of promiscuous CoA-ligase activity 1,3 . While OplR-based biosensors may be able to aid in the selection of mutant acyl-coA ligases with enhanced activity, there remains a sizeable thermodynamic barrier for the cyclization of the 7-membered ring 1 Since naturally-occuring genetically encoded biosensors for chemicals of interest have the potential to be much more sensitive than those repurposed or evolved in the laboratory, it is critical to pursue rapid and efficient means of identifying them.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to being inherently unsustainable, the chemical process to synthesize caprolactam requires toxic reagents and produces unwanted byproducts such as ammonium sulfate 2 . Multiple attempts have been made to produce caprolactam biologically, however the highest titers achieved to date are no greater than 1-2 mg/L 1,3 . All current published strategies to produce caprolactam in vivo rely on the cyclization of 6-aminocaproic acid (6ACA) via a promiscuous acyl-coA ligase 1,3 .…”

Section: Introductionmentioning

confidence: 99%

Identification, characterization, and application of a highly sensitive lactam biosensor fromPseudomonas putida

Thompson

Pearson

Barajas

et al. 2019

Preprint

View full text Add to dashboard Cite

Caprolactam is an important polymer precursor to nylon traditionally derived from petroleum and produced on a scale of 5 million tons per year. Current biological pathways for the production of caprolactam are inefficient with titers not exceeding 2 mg/L, necessitating novel pathways for its production. As development of novel metabolic routes often require thousands of designs and result in low product titers, a highly sensitive biosensor for the final product has the potential to rapidly speed up development times. Here we report a highly sensitive biosensor for valerolactam and caprolactam from Pseudomonas putida KT2440 which is >1000x more sensitive to exogenous ligand than previously reported sensors. Manipulating the expression of the sensor oplR (PP_3516) substantially altered the sensing parameters, with various vectors showing K d values ranging from 700 nM (79.1 μ g/L) to 1.2 mM (135.6 mg/L). Our most sensitive construct was able to detect in vivo production of caprolactam above background at ~6 μ g/L. The high sensitivity and range of OplR is a powerful tool towards the development of novel routes to the biological synthesis of caprolactam.

show abstract

Metabolic engineering of Escherichia coli for the production of four-, five- and six-carbon lactams

Cited by 79 publications

References 29 publications

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

RapidRIP quantifies the intracellular metabolome of 7 industrial strains of E. coli

Identification, characterization, and application of a highly sensitive lactam biosensor fromPseudomonas putida

Contact Info

Product

Resources

About