Metabolic engineering of Enterobacter cloacae for high-yield production of enantiopure (2 R ,3 R )-2,3-butanediol from lignocellulose-derived sugars

Li, Lixiang; Li, Kun; Wang, Yu; Chen, Chao; Xu, Youqiang; Zhang, Lijie; Han, Bin; Gao, Chao; Tao, Fei; Ma, Cuiqing; Xu, Ping

doi:10.1016/j.ymben.2014.11.010

Cited by 126 publications

(80 citation statements)

References 42 publications

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“…Expression of an unmutated galP was able to replace the native phosphotransferase system for glucose in E. coli KJ122 (Jantama et al, 2008b;Zhang et al, 2009;Hernández-Montalvo et al, 2003), restoring growth and succinate production. Overexpression of native galP from a high copy vector increased both glucose and xylose metabolism in Enterobacter cloacea engineered for butanediol production (Li et al, 2015). Mutant strain AS1600a was compared to KJ122 (parent) using sugarcane bagasse as a substrate.…”

Section: A Single Mutation In Galp Is Sufficient To Improve Xylose Mementioning

confidence: 99%

Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

Sawisit

Jantama

Zheng

et al. 2015

Bioresource Technology

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Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation). This strain now ferments 10% xylose poorly. Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose. Clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. One of these, AS1600a, produced 84.26 ± 1.37 g/L succinate, equivalent to that produced by the parent (KJ122) from 10% glucose (85.46 ± 1.78 g/L). AS1600a was sequenced and found to contain a mutation in galactose permease (GalP, G236D). This mutation was shown to be responsible for the improvement in fermentation using KJΔgalP as the host and expression vectors with native galP and with mutant galP(∗). Strain AS1600a and KJΔgalP(pLOI5746; galP(∗)) also co-fermented a mixture of glucose, xylose, arabinose, and galactose in sugarcane bagasse hydrolysate using mineral salts medium.

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Section: A Single Mutation In Galp Is Sufficient To Improve Xylose Mementioning

confidence: 99%

Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

Sawisit

Jantama

Zheng

et al. 2015

Bioresource Technology

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“…Thus, the biotransformation process has been considered as the preferred method for the production of optically pure 2,3-BD (Celinska and Grajek 2009;Ji et al 2011;Zeng and Sabra 2011). Several engineered strains of microorganisms including Saccharomyces cerevisiae, Enterobacter cloacae, Bacillus licheniformis and E. coli have been used for the production of optically pure 2,3-BD (Yan et al 2009;Lian et al 2014;Li et al 2015;Wang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Rahman

Qin

2018

Bioresour. Bioprocess.

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Background:The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate.Results: A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli-K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/ mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. Conclusions:The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli.

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“…This study implemented the following engineering strategy: (1) biosynthesis of undesired 2,3-butanedione stereoisomers was blocked by disrupting a meso-2,3-butanediol dehydrogenase; (2) genes related to the two-carbon catabolite-repression genes PtsG and GalP were deleted, which induced utilization of the lignocellulose-derived sugars glucose and xylose; and (3) genes responsible for enzymes that catalyze the production of 2,3-butanediol derivatives, Ldh and FrdA, were deleted to promote the production of the desired stereoisomer. These three modifications of E. cloacae metabolic pathways resulted in a 16 % increase in (2R,3R)-2,3-butanediol production (with >97 % purity) compared with that of the wild type (Li et al 2015).…”

Section: Engineering 23-butanediol Production In a Model E Colimentioning

confidence: 97%

“…Inactivation of SlaC (to generate the slaC mutant) and overexpression of Bdh (from B. subtilis) in S. marcescens might produce a high level of pure (2R,3R)-2,3-butanediol, but this remains to be tested. A similar approach was used for metabolic engineering of Enterobacter cloacae (Li et al 2015).…”

Section: Engineering 23-butanediol Production In a Model E Colimentioning

confidence: 99%

Sweet scents from good bacteria: Case studies on bacterial volatile compounds for plant growth and immunity

2015

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Beneficial bacteria produce diverse chemical compounds that affect the behavior of other organisms including plants. Bacterial volatile compounds (BVCs) contribute to triggering plant immunity and promoting plant growth. Previous studies investigated changes in plant physiology caused by in vitro application of the identified volatile compounds or the BVC-emitting bacteria. This review collates new information on BVC-mediated plant-bacteria airborne interactions, addresses unresolved questions about the biological relevance of BVCs, and summarizes data on recently identified BVCs that improve plant growth or protection. Recent explorations of bacterial metabolic engineering to alter BVC production using heterologous or endogenous genes are introduced. Molecular genetic approaches can expand the BVC repertoire of beneficial bacteria to target additional beneficial effects, or simply boost the production level of naturally occurring BVCs. The effects of direct BVC application in soil are reviewed and evaluated for potential large-scale field and agricultural applications. Our review of recent BVC data indicates that BVCs have great potential to serve as effective biostimulants and bioprotectants even under open-field conditions.

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Metabolic engineering of Enterobacter cloacae for high-yield production of enantiopure (2 R ,3 R )-2,3-butanediol from lignocellulose-derived sugars

Cited by 126 publications

References 42 publications

Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Sweet scents from good bacteria: Case studies on bacterial volatile compounds for plant growth and immunity

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