Metabolic engineering of Corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction

Kogure, Takahisa; Kubota, Takeshi; Suda, Masako; Hiraga, Kazumi; Inui, Masayuki

doi:10.1016/j.ymben.2016.08.005

Cited by 94 publications

(106 citation statements)

References 65 publications

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“…The possible reason was that the background of the original strain in our study was different with that in the reference, in which the original strain was C. glutamicum ATCC 13032, with deletion of the ldhA , integration of xylose metabolic gene ( xylA , xylB ) and gapA. Overexpression of iolT1 and glk was used to improve glucose consumption and shikimate production in non‐PTS C. glutamicum . In recent studies, IolT2 from Streptomyces coelicolor and ppgk from Bacillus subtilis were overproduced to improve l ‐lysine titer in PTS‐deficient C. glutamicum .…”

Section: Discussionmentioning

confidence: 94%

“…Because non‐PTS system did not consume PEP, the availability of PEP could be increased dramatically, which positively impacted the accumulation of C3 intermediates, such as 3‐phosphoglycerate, and provided more 3‐phosphoglycerate to synthesize its derivatives . The non‐PTS transport system had been successfully exploited in the production of l ‐lysine, succinate, and shikimate . However, the metabolic differences between the PTS and non‐PTS strains and the further influence on the product accumulation have not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Rewiring the Central Metabolic Pathway for High‐Yield l‐Serine Production in Corynebacterium glutamicum by Using Glucose

Zhang

Lai

et al. 2019

Biotechnology Journal

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L-Serine is widely used in the pharmaceutical, food, and cosmetic industries. The direct fermentative production of L-serine from glucose in Corynebacterium glutamicum (C. glutamicum) has been achieved but the yield is generally low. In this study, the central metabolic pathway is rewired to direct higher accumulation of glycerate-3-phosphate (3-PG), the precursor for L-serine biosynthesis, thereby freeing up more carbon to enhance L-serine production and yield. To this end, the native phosphotransferase system (PTS) is inactivated by deleting the gene ptsH. Meanwhile, the PTS-independent glucose uptake pathway is activated via overexpression of iolT1, ppgk, and deletion of the transcriptional regulator gene iolR. Furthermore, metabolic changes between PTS and non-PTS strains are elucidated using gas chromatography-tandem mass spectrometry. Based on the metabonomic and enzyme activities analysis, the glycolysis pathway upstream of 3-PG is further enhanced by co-overexpressing pgi, pfkA, and gapA. The resulting strain with all of the modifications mentioned above leads to the L-serine yield of 0.30 g g −1 glucose (42.8% higher than that of the original strain), which is the highest yield in C. glutamicum by using glucose as the sole carbon source. These strategies can be expanded for the production of other value-added chemicals derived from the intermediates of glycolysis pathway in C. glutamicum.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Rewiring the Central Metabolic Pathway for High‐Yield l‐Serine Production in Corynebacterium glutamicum by Using Glucose

Zhang

Lai

et al. 2019

Biotechnology Journal

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“…C. glutamicum is a nonpathogenic, gram‐positive soil bacterium that has been extensively used for the industrial production of various amino acids (Becker & Wittmann, ; Becker, Rohles, & Wittmann, ; Lee, Na, Kim, Lee, & Kim, ; Wendisch, Jorge, Pérez‐García, & Sgobba, ), and the demand for amino acids is expected to increase in the future (Eggeling & Bott, ). Moreover, C. glutamicum has the potential to produce 1,4‐butanediamine (putrescine) and 1,5‐diaminopentane (DAP; cadaverine) (Buschke et al, ; Buschke, Schröder, & Wittmann, ; Kim et al, ; Kind & Wittmann, ; Kind et al, ; Kind, Kreye, & Wittmann, ; Schneider & Wendisch, ), organic acids (Chen et al, ; Chung, Park, Yun, & Park, ; Wieschalka, Blombach, Bott, & Eikmanns, ), and aromatic compounds (Kogure, Kubota, Suda, Hiraga, & Inui, ; Lee & Wendisch, ). Therefore, this bacterium is promising for use in the production of biofuels and commodity chemicals.…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering to improve 1,5‐diaminopentane production from cellobiose using β‐glucosidase‐secreting Corynebacterium glutamicum

Matsuura

Kishida

Konishi

et al. 2019

Biotech & Bioengineering

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Microbial production of 1,5-diaminopentane (DAP) from renewable feedstock is a promising and sustainable approach for the production of polyamides. In this study, we constructed a β-glucosidase (BGL)-secreting Corynebacterium glutamicum and successfully used this strain to produce DAP from cellobiose and glucose. First, C. glutamicum was metabolically engineered to produce L-lysine (a direct precursor of DAP), followed by the coexpression of L-lysine decarboxylase and BGL derived from Escherichia coli and Thermobifida fusca YX (Tfu0937), respectively. This new engineered C. glutamicum strain produced 27 g/L of DAP from cellobiose in CGXII minimal medium using fed-batch cultivation. The yield of DAP was 0.43 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose), which is the highest yield reported to date. These results demonstrate the feasibility of DAP production from cellobiose or cellooligosaccharides using an engineered C. glutamicum strain.

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“…Shikimic acid (3, 4, 5‐tri‐hydroxy‐1‐cyclohexene‐1‐carboxylic acid) is an important biochemical metabolite in plants and microorganisms . Many potent characteristics including high functionalization, six‐carbon cyclitol with three chiral carbons and a carboxylic acid functional group offer versatility to shikimic acid.…”

Section: Introductionmentioning

confidence: 99%

“…To date, shikimate pathway engineering has been widely practiced by several researchers, with Escherichia coli presenting the most advantages . Phosphoenolpyruvate availability particularly displays a key bottleneck in the induced shikimate production and production of other aromatic compounds . One widely accepted possible reason for this limitation is metabolic competition among DAHP synthase and numerous PEP‐exhausting activities involved in central carbon metabolism .…”

Section: Introductionmentioning

confidence: 99%

Systematically engineering Escherichia coli for enhanced shikimate biosynthesis co‐utilizing glycerol and glucose

Bilal

Yue

et al. 2018

Biofuels Bioprod Bioref

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In this study, an Escherichia coli strain with the capability to utilize glycerol/glucose simultaneously was genetically engineered to produce high shikimate. To achieve this, the ptsG gene encoding EIICBglc protein was inactivated in the BW25113 strain to alleviate carbon catabolite repression. The shikimate kinase genes aroK and aroL were also eliminated to construct the SA-B2 strain, which led to a 42.3% increase in shikimate production. Thereafter, three critical genes, namely mutant aroG fbr , ppsA, and tktA, were overexpressed in strain SA-B2, and the resulting engineered SA-B7 strain produced 0.92 g/L shikimate in shake flask cultures, which were 2.5 times higher than strain SA-B2. The influence of varying carbon mole numbers ratios of glycerol to glucose on the production of shikimate was also investigated, where the highest shikimate production was attained at a glycerolto-glucose ratio of 12:1 by SA-B7. Moreover, the amount of NADPH was 3.5-times higher in the mixed culture medium as compared to sole glycerol-based medium. RT-PCR data indicated that the addition of glucose upregulated the transcriptional levels of genes pertaining to shikimate biosynthesis. In conclusion, the findings demonstrated that E. coli could be modified as a potential cell factory to produce shikimate and other high-value derived aromatic compounds by genetic and metabolic engineering strategies.Spotlight: Engineering E. coli for enhanced shikimate biosynthesis M Bilal et al..

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Metabolic engineering of Corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction

Cited by 94 publications

References 65 publications

Rewiring the Central Metabolic Pathway for High‐Yield l‐Serine Production in Corynebacterium glutamicum by Using Glucose

Rewiring the Central Metabolic Pathway for High‐Yield l‐Serine Production in Corynebacterium glutamicum by Using Glucose

Metabolic engineering to improve 1,5‐diaminopentane production from cellobiose using β‐glucosidase‐secreting Corynebacterium glutamicum

Systematically engineering Escherichia coli for enhanced shikimate biosynthesis co‐utilizing glycerol and glucose

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