Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

Borja, Gheorghe M.; Rodriguez, Angelica; Campbell, Kate; Borodina, Irina; Chen, Yun; Nielsen, Jens

doi:10.1186/s12934-019-1244-4

Cited by 34 publications

(32 citation statements)

References 71 publications

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“…In recent years, some research effort has been put into replacing the hydrocarbon source for these fermentation processes by residues and waste materials (Vargas-Tah and Gosset, 2015;Martínez-Avila et al, 2018;Borja et al, 2019). Sugars like glucose are abundant low-cost raw materials.…”

Section: Discussionmentioning

confidence: 99%

Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges

Braga

Faria

2020

Front. Bioeng. Biotechnol.

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The most common route to produce aromatic chemicals-organic compounds containing at least one benzene ring in their structure-is chemical synthesis. These processes, usually starting from an extracted fossil oil molecule such as benzene, toluene, or xylene, are highly environmentally unfriendly due to the use of nonrenewable raw materials, high energy consumption and the usual production of toxic by-products. An alternative way to produce aromatic compounds is extraction from plants. These extractions typically have a low yield and a high purification cost. This motivates the search for alternative platforms to produce aromatic compounds through low-cost and environmentally friendly processes. Microorganisms are able to synthesize aromatic amino acids through the shikimate pathway. The construction of microbial cell factories able to produce the desired molecule from renewable feedstock becomes a promising alternative. This review article focuses on the recent advances in microbial production of aromatic products, with a special emphasis on metabolic engineering strategies, as well as bioprocess optimization. The recent combination of these two techniques has resulted in the development of several alternative processes to produce phenylpropanoids, aromatic alcohols, phenolic aldehydes, and others. Chemical species that were unavailable for human consumption due to the high cost and/or high environmental impact of their production, have now become accessible.

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Section: Discussionmentioning

confidence: 99%

Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges

Braga

Faria

2020

Front. Bioeng. Biotechnol.

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“…Quantification of glucose, glycerol and organic acids was carried out on Aminex HPX-87H column (Bio-Rad Laboratories, USA) as described before ( Borja et al, 2019 ), but with a column temperature of 60 °C.…”

Section: Methodsmentioning

confidence: 99%

Engineering the oleaginous yeast Yarrowia lipolytica for high-level resveratrol production

Sáez-Sáez

Wang

Marella

et al. 2020

Metabolic Engineering

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Resveratrol is a plant secondary metabolite with multiple health-beneficial properties. Microbial production of resveratrol in model microorganisms requires extensive engineering to reach commercially viable levels. Here, we explored the potential of the non-conventional yeast Yarrowia lipolytica to produce resveratrol and several other shikimate pathway-derived metabolites ( p -coumaric acid, cis , cis -muconic acid, and salicylic acid). The Y. lipolytica strain expressing a heterologous pathway produced 52.1 ± 1.2 mg/L resveratrol in a small-scale cultivation. The titer increased to 409.0 ± 1.2 mg/L when the strain was further engineered with feedback-insensitive alleles of the key genes in the shikimate pathway and with five additional copies of the heterologous biosynthetic genes. In controlled fed-batch bioreactor, the strain produced 12.4 ± 0.3 g/L resveratrol, the highest reported titer to date for de novo resveratrol production, with a yield on glucose of 54.4 ± 1.6 mg/g and a productivity of 0.14 ± 0.01 g/L/h. The study showed that Y. lipolytica is an attractive host organism for the production of resveratrol and possibly other shikimate-pathway derived metabolites.

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“…Opposite reactions incorporate loss of hunger, sickness or heaving, expanded hair development, and shuddering or trembling of the hand. The particular immunosuppressive treatment is expected to lessen the high bleakness because of diseases, malignancies additionally, and join misfortune because of incessant dismissal after kidney transplantation [5][6][7].…”

Section: Methodsmentioning

confidence: 99%

Regulation and expression of immunosuppressants compounds from a microbial source

Chandrudu¹,

Sagar²,

Madhubhushan³

et al. 2020

irjpas

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As of late, numerous immunosuppressive medications have been found and created for clinical usage in relocation, to instigate immunosuppression. Immunosuppressants may any be exogenous, as immunosuppressive medications, or endogenous, as testosterone. They are utilized in immunosuppressive treatment to forestall the dismissal of relocated tissues and organs. It is likewise used to treat immune system sicknesses or illnesses that are undoubted of immune system source and some other non-immune system provocative ailments (e.g., long haul hypersensitive asthma control). The more significant part of these immunosuppressive medications act non-specifically, the insusceptible framework is less ready to oppose diseases and the spread of dangerous cells. The revelation of immunosuppressant drugs, lately, welcomed bewildering sway on clinical treatment as a powerful amalgamated to achieve fruitful organ transplantation. Immunosuppressants are substances that stifle the invulnerable reaction, either by diminishing the actuation or adequacy of the safe framework. The separation and portrayal of immunosuppressants from non-pathogenic organisms disengaged various tests of Chennai were gathered. The parasitic development was watched visibly, infinitesimally, and the morphology was watched. Antifungal action of separated contagious strain was broke down by Paper Disk Agar Diffusion Method using the creation of auxiliary metabolites. SDS-PAGE affirmed the presence of discharged protein.

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Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

Cited by 34 publications

References 71 publications

Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges

Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges

Engineering the oleaginous yeast Yarrowia lipolytica for high-level resveratrol production

Regulation and expression of immunosuppressants compounds from a microbial source

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