Metabolic engineering and synthetic biology employing Lactococcus lactis and Bacillus subtilis cell factories

Tilburg, Amanda Y van; Cao, Heng; Meulen, Sjoerd B van der; Solopova, Ana; Kuipers, Oscar P.

doi:10.1016/j.copbio.2019.01.007

Cited by 32 publications

(34 citation statements)

References 62 publications

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“…B. subtilis has become the most studied species of gram-positive bacteria and is largely exploited as a microbial cell factory [50]. The full B. subtilis genome was sequenced in 1997 [51], and its annotation is reported in several dedicated databases, such as 'SubtiWiki' and 'BSubCyc'.…”

Section: Intracellular Metabolite Levels In Bacillus Subtilis Are Higmentioning

confidence: 99%

Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems

et al. 2020

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Absolute quantification of intracellular metabolite pools is a prerequisite for modeling and in-depth biological interpretation of metabolomics data. It is the final step of an elaborate metabolomics workflow, with challenges associated with all steps—from sampling to quantifying the physicochemically diverse metabolite pool. Chromatographic separation combined with mass spectrometric (MS) detection is the superior platform for high coverage, selective, and sensitive detection of metabolites. Herein, we apply our quantitative MS-metabolomics workflow to measure and present the central carbon metabolome of a panel of commonly applied biological model systems. The workflow includes three chromatographic methods combined with isotope dilution tandem mass spectrometry to allow for absolute quantification of 68 metabolites of glycolysis, the pentose phosphate pathway, the tricarboxylic acid cycle, and the amino acid and (deoxy) nucleoside pools. The biological model systems; Bacillus subtilis, Saccharomyces cerevisiae, two microalgal species, and four human cell lines were all cultured in commonly applied culture media and sampled in exponential growth phase. Both literature and databases are scarce with comprehensive metabolite datasets, and existing entries range over several orders of magnitude. The workflow and metabolite panel presented herein can be employed to expand the list of reference metabolomes, as encouraged by the metabolomics community, in a continued effort to develop and refine high-quality quantitative metabolomics workflows.

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Section: Intracellular Metabolite Levels In Bacillus Subtilis Are Higmentioning

confidence: 99%

Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems

et al. 2020

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“…However, not only Gram-negative bacteria are popular hosts for SynBio. Gram-positive organisms, such as B. subtilis and L. lactis , are excellent producers and in vivo delivery vehicles for therapeutic products, including oral vaccines 6 . This is due to their lack of immunogenic lipopolysaccharides and their ‘Generally Regarded As Safe’ (GRAS) status conferred by the FDA 6 .…”

Section: Rising Stars In the Field Of Synthetic Biologymentioning

confidence: 99%

“…Gram-positive organisms, such as B. subtilis and L. lactis , are excellent producers and in vivo delivery vehicles for therapeutic products, including oral vaccines 6 . This is due to their lack of immunogenic lipopolysaccharides and their ‘Generally Regarded As Safe’ (GRAS) status conferred by the FDA 6 . In addition, B. subtilis shows good growth and robustness in industrial fermentations and possesses a superior capacity of protein secretion in comparison to other hosts, whereas L. lactis has become indispensable in the dairy industry as a fermenter and producer of flavouring and texturizing compounds 6 , 7 .…”

Section: Rising Stars In the Field Of Synthetic Biologymentioning

confidence: 99%

“…This is due to their lack of immunogenic lipopolysaccharides and their ‘Generally Regarded As Safe’ (GRAS) status conferred by the FDA 6 . In addition, B. subtilis shows good growth and robustness in industrial fermentations and possesses a superior capacity of protein secretion in comparison to other hosts, whereas L. lactis has become indispensable in the dairy industry as a fermenter and producer of flavouring and texturizing compounds 6 , 7 . Furthermore, other lesser-known Gram-positive bacteria show interesting features for SynBio applications.…”

Section: Rising Stars In the Field Of Synthetic Biologymentioning

confidence: 99%

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Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

2020

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Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have unique and versatile metabolisms, offering unique opportunities for Synthetic Biology (SynBio). However, key genome editing and recombineering tools require optimization and large-scale multiplexing to unlock the full SynBio potential of these bacteria. In addition, the limited availability of a set of characterized, species-specific biological parts hampers the construction of reliable genetic circuitry. Mining of currently available, diverse bacteriophages could complete the SynBio toolbox, as they constitute an unexplored treasure trove for fully adapted metabolic modulators and orthogonally-functioning parts, driven by the longstanding co-evolution between phage and host.

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“…Escherichia coli and Saccharomyces cerevisiae are the most commonly employed microorganisms for aromatic compound production. However, more recently, other hosts have also been explored due to their peculiarities, such as Corynebacterium glutamicum, Lactococcus lactis, Pseudomonas putida, and Streptomyces lividans (Sachan et al, 2006;Gosset, 2009;Verhoef et al, 2009;Gaspar et al, 2016;Kallscheuer et al, 2016Kallscheuer et al, , 2019Dudnik et al, 2017Dudnik et al, , 2018Braga et al, 2018a;Tilburg et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges

Braga

Faria

2020

Front. Bioeng. Biotechnol.

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The most common route to produce aromatic chemicals-organic compounds containing at least one benzene ring in their structure-is chemical synthesis. These processes, usually starting from an extracted fossil oil molecule such as benzene, toluene, or xylene, are highly environmentally unfriendly due to the use of nonrenewable raw materials, high energy consumption and the usual production of toxic by-products. An alternative way to produce aromatic compounds is extraction from plants. These extractions typically have a low yield and a high purification cost. This motivates the search for alternative platforms to produce aromatic compounds through low-cost and environmentally friendly processes. Microorganisms are able to synthesize aromatic amino acids through the shikimate pathway. The construction of microbial cell factories able to produce the desired molecule from renewable feedstock becomes a promising alternative. This review article focuses on the recent advances in microbial production of aromatic products, with a special emphasis on metabolic engineering strategies, as well as bioprocess optimization. The recent combination of these two techniques has resulted in the development of several alternative processes to produce phenylpropanoids, aromatic alcohols, phenolic aldehydes, and others. Chemical species that were unavailable for human consumption due to the high cost and/or high environmental impact of their production, have now become accessible.

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Metabolic engineering and synthetic biology employing Lactococcus lactis and Bacillus subtilis cell factories

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Cited by 32 publications

References 62 publications

Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems

Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems

Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges

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