Metabolic effects of Hedyotis diffusa on rats bearing Walker 256 tumor revealed by NMR‐based metabolomics

Abstract

Hedyotis diffusa, a traditional Chinese herbal medicine, is widely used for oncotherapy and shows a positive effect in the clinical treatment. But its mechanism of anticancer activities is complicated and unclear. This study was undertaken to assess the therapeutic effects and reveal detailed mechanisms of H. diffusa for oncotherapy. A Walker 256 tumor-bearing rat model was established, and metabolomic profiles of plasma and urine were obtained from 1 H NMR technique. Multivariate statistical analysis methods were used to characterize the discriminating metabolites between control (C), Walker 256 tumorbearing rats model (M), and H. diffusa treatment (H) groups. Finally, 13 and 10 metabolomic biomarkers in urine and plasma samples were further identified as characteristic metabolites in M group, whereas H group showed a partial metabolic balance recovered, such as ornithine, N-acetyl-L-aspartate, L-aspartate, and creatinine in urine samples, and acetate, lactate, choline, L-glutamine, and 3-hydroxybutyrate in plasma samples. On the basis of the methods above, we hypothesized H. diffusa treatment reduced the injury caused by Walker 256 tumor and maintained a metabolic balance. Our study demonstrated that this method provided new insights into metabolic alterations in tumor-bearing biosystems and researching on the effects of H. diffusa on the endogenous metabolism in tumor-bearing rats.

Metabolic regulations of a decoction of Hedyotis diffusa in acute liver injury of mouse models

“…The latest new in anticancer study suggests that H. diffusa treatment is capable of reducing the injury caused by Walker 256 tumor and maintaining a metabolic balance [ 18 ]. In addition, recent evidence reveals that the dysfunctional metabolisms are responsible for LPS/D/GALN-induced acute hepatitis [ 30 , 31 ].…”

“…Actually, recent papers coincidentally demonstrate that inflammatory process, cancer development and progression, and immune response have strong inter-relationship with metabolism happened in host cells [ 14 – 17 ]. Currently, only one paper focuses on metabolic alterations of H. diffusa in tumor-bearing rat [ 18 ]. The underlying metabolic mechanisms related to H. diffusa -involved activities need to be elaborated further, and the metabolic activities of this herb in liver inflammation is unknown.…”

Metabolomic Profiling of the Synergistic Effects of Ginsenoside Rg1 in Combination with Neural Stem Cell Transplantation in Ischemic Stroke Rats

“…To explore the metabolites that were responsible for the separation, the OPLS-DA score plots, permutation tests results, cross-validated scores plots, and corresponding loading plots of serum samples were generated from three groups (Figure and S3) to figure out the key metabolites for the intergroup differences. The parameters from the model and sham group were R 2 Y = 96.8%, Q 2 Y = 0.654, from the Rg1 treated group and model group were R 2 Y = 93.5%, Q 2 Y = 0.05, from the NSCs treated group and model group were R 2 Y = 95.5%, Q 2 Y = 0.398, and from the Rg1+NSCs-treated group and model group were R 2 Y = 94.4%, Q 2 Y = 0.251 in the OPLS-DA model, respectively. − The quality of the models was further assessed with CV-ANOVA, which resulted in a score of p = 0.015, indicating that the differences between model and sham group within the OPLS-DA model were significant . In contrast, the result of the CV-ANOVA suggests that the OPLS-DA models comparing the model group with the Rg1+NSCs-treated group were marginally significant, having p -value of 0.071.…”

“…To explore the metabolites that were responsible for the separation, the OPLS-DA score plots, permutation tests results, cross-validated scores plots, and corresponding loading plots of 38−40 The quality of the models was further assessed with CV-ANOVA, which resulted in a score of p = 0.015, indicating that the differences between model and sham group within the OPLS-DA model were significant. 41 In contrast, the result of the CV-ANOVA suggests that the OPLS-DA models comparing the model group with the Rg1+NSCstreated group were marginally significant, having p-value of 0.071. So we used an a priori cutoff VIP value >1 to reduce the data set in the model, based on the literature.…”

Chinese Medicines for Cancer Treatment from the Metabolomics Perspective

“…Hedyotis diffusa is a famous Chinese herbal medicine with antineoplastic property. To predict the potential underlying mechanism, a 1 H NMR-based metabolomics was conducted to use plasma and urine from rats bearing Walker 256 tumor [78]. Hedyotis diffusa treatment was found to reverse lactate, acetate, choline, 3-hydroxybutyrate, and L-glutamine in plasma as well as creatinine, L-aspartate, N-acetyl-L-aspartate, and ornithine in urine.…”