Metabolic effects of exercise in the golden fish Salminus maxillosus "dourado" (Valenciennes, 1849)

Moraes, Gilberto; Choudhuri, Jomuna V.; Souza, R.H.S; Neto, Chiara

doi:10.1590/s1519-69842004000400013

Cited by 10 publications

(5 citation statements)

References 14 publications

(16 reference statements)

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“…Glucose was measured from the serum, testis samples, and media using the kit obtained from Span Diagnostics Ltd., Mumbai India by quantitative calorimetric method (Moares et al, ). Ten percent tissue homogenate (w/v) was prepared in 6N Perchloric acid (PCA) under ice bath.…”

Section: Materials and Methodologymentioning

confidence: 99%

Testicular glucose and its transporter GLUT 8 as a marker of age‐dependent variation and its role in steroidogenesis in mice

et al. 2014

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The present study evaluates the hypothesis, that glucose is essential for steroidogenesis and inadequate supply of glucose to the testis may be responsible for decline in steroidogenesis in mice during aging. Mice of different age groups (birth, weaning, puberty, reproductively active, and senescence) were utilized for this study. The changes in glucose, glucose transporter (GLUT), and insulin receptor (IR) level in the testis were evaluated and compared with the testicular steroidogenic parameters such as steroidogenic acute regulatory protein (StAR), 3β-hydroxy steroid dehydrogenase (3β-HSD) and circulating testosterone levels. The result showed significant correlation between changes in GLUT 8 and glucose levels with changes in StAR level in the testis and circulating testosterone level in the mice from birth to senescence. Immunohistochemical analysis showed intense immunostaining of GLUT 8 and IR in the interstitial cells, most likely Leydig cells, in testis of pubertal and reproductively active mice suggesting their relevance in steroidogenesis. The in vitro study showed a significant positive correlation between luteinizing hormone (LH)-induced increase in GLUT 8 and StAR (r = 0.82; P< 0.05) proteins level in the testes with increase in testosterone (r = 0.97; P < 0.05) synthesis of reproductively active mice. This study also showed increased release of lactate with increased uptake of glucose by the testis. Further, intra-testicular treatment of 2-deoxy glucose, to reproductively active mice caused a significant decrease in 3β-HSD enzyme activity in the testis. Based on these findings, it may be concluded that the changes in glucose level either directly or indirectly lead to changes in testicular steroidogenesis during aging.

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Section: Materials and Methodologymentioning

confidence: 99%

Testicular glucose and its transporter GLUT 8 as a marker of age‐dependent variation and its role in steroidogenesis in mice

et al. 2014

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“…Serum, STf and ITf glucose content was measured using an enzymatic (Mutarotase-GOD) calorimetric technique (Autokit Glucose Wako, Wako, TX, USA)) according to the manufacturer's instructions. Tissue factions were prepared as described by Koya et al [49] with some modifications. Briefly, STf and ITf were sonicated in 6 N perchloric acid while in an ice bath.…”

Section: Serum and Tissue Glucose Measurementsmentioning

confidence: 99%

Glucose, insulin, insulin receptor subunits α and β in normal and spontaneously diabetic and obese ob/ob and db/db infertile mouse testis and hypophysis

Pelletier

Layeghkhavidaki

Vitale

2020

Reprod Biol Endocrinol

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Background: Type 2 diabetes touches young subjects of reproductive age in epidemic proportion. This study assesses glucose, total InsulinT, Insulin2 and insulin receptor subunits α and β in testis during mouse development then, in the spontaneously type 2 diabetes models associated with infertility db/db and ob/ob mice. IR-β and α were also assessed in spermatozoa (SPZ), anterior pituitary (AP) and serum. Methods: Serum and tissue glucose were measured with enzymatic colorimetric assays and InsulinT and Insulin2 by ELISAs in serum, interstitial tissue-(ITf) and seminiferous tubule (STf) fractions in14-> 60-day-old normal and db/ db, ob/ob and wild type (WT) mice. IR subunits were assessed by immunoblotting in tissues and by immunoprecipitation followed by immunoblotting in serum. Results: Development: Glucose increased in serum, ITf and STf. InsulinT and Insulin2 dropped in serum; both were higher in STf than in ITf. In > 60-day-old mouse ITf, insulinT rose whereas Insulin2 decreased; InsulinT and Insulin2 rose concurrently in STf. Glucose and insulin were high in > 60-day-old ITf; in STf high insulin2 accompanied low glucose. One hundred ten kDa IR-β peaked in 28-day-old ITf and 14-day-old STf. One hundred thirty five kDa IR-α was high in ITf but decreased in STf. Glucose escalated in db/db and ob/ob sera. Glucose doubled in ITf while being halved in STf in db/db mice. Glucose significantly dropped in db/db and ob/ob mice spermatozoa. InsulinT and Insulin2 rose significantly in the serum, ITf and STf in db/db and ob/ob mice. One hundred ten kDa IR-β and 135 kDa IR-α decreased in db/db and ob/ob ITf. Only 110 kDa IR-β dropped in db/db and ob/ob STf and AP. One hundred ten kDa IR-β fell in db/db and ob/ob SPZ. One hundred ten kDa sIR-α rose in the db/db and ob/ob mouse sera.

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“…A colorimetric estimation of glucose concentration in testis samples was performed by using specific commercial kit according to the protocol of Moraes et al . () with minor modifications (Verma & Krishna, ). In brief, 10% (w/v) tissue homogenate was made in 6 N perchloric acid (PCA) in an ice bath.…”

Section: Methodsmentioning

confidence: 97%

Neonatal hypothyroidism affects testicular glucose homeostasis through increased oxidative stress in prepubertal mice: effects on GLUT3, GLUT8 and Cx43

Sarkar

Singh

2017

Andrology

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Thyroid hormones (THs) play an important role in maintaining the link between metabolism and reproduction and the altered THs status is associated with induction of oxidative stress in various organs like brain, heart, liver and testis. Further, reactive oxygen species play a pivotal role in regulation of glucose homeostasis in several organs, and glucose utilization by Leydig cells is essential for testosterone biosynthesis and thus is largely dependent on glucose transporter 8 (GLUT8). Glucose uptake by Sertoli cells is mediated through glucose transporter 3 (GLUT3) under the influence of THs to meet energy requirement of developing germ cells. THs also modulate level of gap junctional protein such as connexin 43 (Cx43), a potential regulator of cell proliferation and apoptosis in the seminiferous epithelium. Although the role of transient neonatal hypothyroidism in adult testis in terms of testosterone production is well documented, the effect of THs deficiency in early developmental period and its role in testicular glucose homeostasis and oxidative stress with reference to Cx43 in immature mice remain unknown. Therefore, the present study was conducted to evaluate the effect of neonatal hypothyroidism on testicular glucose homeostasis and oxidative stress at postnatal days (PND) 21 and 28 in relation to GLUT3, GLUT8 and Cx43. Hypothyroidism induced by 6-propyl-2-thiouracil (PTU) markedly decreased testicular glucose level with considerable reduction in expression level of GLUT3 and GLUT8. Likewise, lactate dehydrogenase (LDH) activity and intratesticular concentration of lactate were also decreased in hypothyroid mice. There was also a rise in germ cell apoptosis with increased expression of caspase-3 in PTU-treated mice. Further, neonatal hypothyroidism affected germ cell proliferation with decreased expression of proliferating cell nuclear antigen (PCNA) and Cx43. In conclusion, our results suggest that neonatal hypothyroidism alters testicular glucose homeostasis via increased oxidative stress in prepubertal mice, thereby affecting germ cell survival and proliferation.

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Metabolic effects of exercise in the golden fish Salminus maxillosus "dourado" (Valenciennes, 1849)

Cited by 10 publications

References 14 publications

Testicular glucose and its transporter GLUT 8 as a marker of age‐dependent variation and its role in steroidogenesis in mice

Testicular glucose and its transporter GLUT 8 as a marker of age‐dependent variation and its role in steroidogenesis in mice

Glucose, insulin, insulin receptor subunits α and β in normal and spontaneously diabetic and obese ob/ob and db/db infertile mouse testis and hypophysis

Neonatal hypothyroidism affects testicular glucose homeostasis through increased oxidative stress in prepubertal mice: effects on GLUT3, GLUT8 and Cx43

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