Metabolic effects of dietary conjugated linoleic acid (CLA) isomers in rats

Akahoshi, Asuka; Koba, Kazunori; Ohkura-Kaku, Shihoko; Kaneda, Naoko; Goto, Chikage; Sano, Hiroe; Iwata, Toshio; Yamauchi, Yoshie; Tsutsumi, K.; Sugano, Michihiro

doi:10.1016/j.nutres.2003.08.004

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…On the other hand, recent investigations that have not documented a considerable reduction in BWG are usually those in which low concentrations of CLA isomers were used in diets (≤0.5%) or the dietary CLA isomer mixtures contained lower amounts of t10c12CLA (Sisk et al, 2001). Our results are also supported by several other investigations, which have indicated that the t10c12 isomer, due to its positional and geometric property, is the most potent CLA isomer in terms of antiobesity activity, thus the one which most effi ciently reduces feed intake (Czauderna et al, 2004a), body weight gain (Akahoshi et al, 2003(Akahoshi et al, , 2004, concentration of plasma leptin and the abundance of sterol regulatory element-binding protein mRNA (Wang et al, 2004).…”

Section: Effects Of the Dietary Cla Isomer Mixtures Se VI And Se-y Osupporting

confidence: 89%

“…Thus, our results are in agreement with the studies of Alasnier et al (2002) in which t10c12CLA and t10t12CLA were also more effi ciently driven through β-oxidation in the cells of the liver, muscles, kidneys or adipose tissue compared with their 9,11 homologues. Moreover, based on the concentration sums of all assayed fatty acids (ΣFA) in the liver and muscles (Tables 2 and 3) and other studies (Alasnier et al, 2002;Akahoshi et al, 2003), we suggest that CLAmix A and CLAmix B added to the rat diet stimulated FA oxidation in rats.…”

Section: The Infl Uence Of the Diets On The Concentration Of Saturatementioning

confidence: 51%

“…Numerous benefi cial regulatory effects of CLA isomers on immune functions, cytokines, immunoglobulin production, the ratio of protein to fat in mammal bodies (repartition), lipids and eicosanoid metabolism have been reported (Akahoshi et al, 2003(Akahoshi et al, , 2004Turpeinen et al, 2006). Moreover, CLA isomers can modulate the expression of numerous genes, either directly or through specifi c transcription factors involved in the many metabolic processes that they affect (Raes at al., 2004;Wahle et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the influence of different chemical forms of selenium and the profiles of CLA isomers in the diet on the fatty acid and amino acid contents in the liver and femoral muscles of rats

Czauderna¹,

Kowalczyk²,

Korniluk³

et al. 2007

J. Anim. Feed Sci.

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The infl uence of diets enriched in 2% CLA isomer mixture (as CLAmix A or CLAmix B ) and/or Se as selenate (Se VI ) or selenized yeast (Se-Y) on the concentration of fatty acids (FA) and amino acids (AA) in the liver and femoral muscles was studied on 8 groups of 7-8 rats aged 8 weeks. Rats were fed a basal diet for 29 days or diets enriched with two combinations of 2% CLAmix A with/without 2 ppm Se (as Se VI ) or 2% CLAmix B with/without 1.2 ppm Se (as Se-Y). The dietary CLAmix B containing the higher concentration of trans10cis12CLA (t10c12CLA) resulted in a higher decrease of body weight gain (BWG) (P<0.1) than the dietary CLAmix A with the lower concentration of t10c12CLA. The diet enriched in Se VI reduced the BWG (P<0.05) in rats. The dietary CLAmix A lowered the concentration of saturated FA (SFA), atherogenic SFA and thrombogenic SFA in the liver and muscles. The value of the Δ9-desaturase index decreased in the liver and muscles of rats fed the diet enriched in CLAmix A or CLAmix B compared with that in the control rats. There were signifi cantly higher (P<0.01) concentrations of c9t11CLA, t10c12CLA, ccCLA and ttCLA in the muscles compared with the concentrations of these isomers in the liver of rats fed the diets enriched in CLAmix A or CLAmix B , regardless the presence of extra Se as Se VI or Se-Y. The ratio (R t10c12/c9t11 ) of t10c12CLA and c9t11CLA in the liver and muscles of rats fed the diets enriched in CLA isomers was, regardless of the presence of Se, smaller compared with the values of R t10c12/c9t11 in the dietary CLA isomers. The concentrations of CLA isomers tended to or signifi cantly increased in the liver and muscles of rats fed diets containing Se and CLA isomers compared with rats fed the diets enriched in CLA isomers. The concentrations of PUFAn-3, PUFAn-6, PUFA and the ratio of PUFA/SFA decreased in the muscles of rats fed the diet with CLAmix A . The results suggest that the dietary CLAmix A containing the higher concentration of ttCLA, regardless of the presence of Se VI , more effi ciently reduced the magnitude of lipoprotein synthesis in muscles than the diet containing CLAmix B with/without Se-Y, Journal of Animal and Feed Sciences, 16, 2007, 678-695 while dietary Se-Y most effectively reduced the anti-obesity properties of CLA isomers in rats. The diets enriched in CLA isomers (as CLAmix A or CLAmix B ), regardless of the presence of Se VI , led to a decrease in the concentration of methionine (Met) in the liver and the concentration of all amino acids in the muscles, while increasing the concentration of Met in muscles.

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Section: Effects Of the Dietary Cla Isomer Mixtures Se VI And Se-y Osupporting

confidence: 89%

Section: The Infl Uence Of the Diets On The Concentration Of Saturatementioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the influence of different chemical forms of selenium and the profiles of CLA isomers in the diet on the fatty acid and amino acid contents in the liver and femoral muscles of rats

Czauderna¹,

Kowalczyk²,

Korniluk³

et al. 2007

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…At the mitochondrial level, a greater part of activated fatty acids was directed from triacylglycerol esterification towards oxidation. Interestingly, t10c12CLA is much more effective at lowering feed intake than the c9t11CLA isomer (Akahoshi et al, 2003), t10c12CLA appeared to be the effective isomer for modulating gene expression in, e.g., cultured 3T3-L1 pre-adipocytes (Choi et al, 2001). Consequently, no decrease in feed intake was observed when the diet was enriched only in the c9t11 isomer, while administration of this isomer numerically increased body weight gain and feed conversion efficiency (FCE) compared with control rats.…”

Section: Resultsmentioning

confidence: 99%

Influence of dietary conjugated linoleic acid isomers and selenium on growth, feed efficiency, and liver fatty acid profile in rats

Korniluk¹,

Czauderna²,

Kowalczyk³

et al. 2006

J. Anim. Feed Sci.

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The influence of dietary CLA isomer(s) and/or selenized yeast on the growth, concentration of CLA isomers and other fatty acids in the liver was investigated in rats. Plasma blood triacylglycerols (TAG), total cholesterol (TC), HDL and LDL cholesterol fractions in relation to dietary CLA isomer(s) and/or selenium (Se) were analysed. The experiment was performed on female rats (Wistar), 8 weeks of age and initial body weight of about 200 g. After a 1-week preliminary period, for 4 weeks the animals were fed a diet enriched in conjugated linoleic acid (CLA) isomer(s) and selenized yeast (2×2 experimental design). Dietary Se or/and CLA isomer(s) resulted in small changes in the spleen, heart, kidneys and brain, and increased liver weight. Administration of Se and trans10cis12CLA most efficiently increased the body weight gain of rats. CLA isomer(s) administered with or without Se elevated the CLA isomer(s) level in the liver. These results demonstrate that trans,transCLA isomers are metabolized more slowly, while cis,trans/trans,cisCLA isomers, more rapidly to longchain fatty acids containing a conjugated double bond. Enrichment of the diet in CLA isomer(s) with or without Se caused a reduction in the capacity of Δ9-, Δ6-and Δ5-desaturases in the liver, while dietary trans10cis12CLA or the CLA isomer mixture increased Δ4-desaturases. The contents of oleic acid, C20:4n-6, and C20:5n-3 decreased in the liver, whereas the level of C22:5n-3 and C22:6n-3 increased in the liver of rats fed the CLA isomer mixture. Individual dietary CLA isomers with or without Se increased the concentration of palmitic and stearic acids in the liver. All experimental diets increased the concentration of triacylglycerols in blood plasma, while trans10cis12CLA with or without Se usually decreased the concentration of total cholesterol, LDL, and HDL cholesterol.

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“…Previous reports have suggested that CLA has no effect on food intake or food efficiency in animals fed a normal diet, but that it significantly inhibits body weight gain in animals fed a highfat diet. 27,28 Together, these results show that N-CLA is more efficient than CLA in reducing body weight.…”

Section: Resultsmentioning

confidence: 69%

Bioavailability of nanoemulsified conjugated linoleic acid for an antiobesity effect

Kim¹,

Park²,

Kweon³

et al. 2013

IJN

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Background:The aim of this study was to enhance the bioavailability of conjugated linoleic acid (CLA), which has low water solubility, using nanoemulsion technology and to evaluate the effects of its improved bioavailability as an antiobesity agent. Methods: The antiobesity effect of nanoemulsified water-soluble conjugated linoleic acid (N-CLA) was evaluated using in vitro and in vivo studies. Differentiated 3T3-L1 adipocytes were treated with CLA and N-CLA to assess their lipolytic effect. Further, to confirm the antiobesity effect of N-CLA, male Sprague-Dawley rats were randomly separated into four groups, ie, a group fed a normal diet, a group fed a high-fat diet (obesity rat model), a CLA-treated group, and an N-CLA-treated group. Results: N-CLA showed a greater lipolytic effect on differentiated 3T3-L1 adipocytes compared with normal CLA. N-CLA enhanced the release of glycerol from triglycerides, which accumulated in differentiated 3T3-L1 adipocytes. Further, N-CLA enhanced leptin secretion to an extent similar to that of orlistat, an antiobesity agent. In an animal obesity model fed a high-fat diet, N-CLA attenuated accumulation of triglycerides, total cholesterol, and low-density lipoprotein cholesterol in serum, and also significantly decreased the volume of triglycerides and cholesterol in liver tissue. Conclusion: These results indicate that N-CLA has a greater antiobesity effect than CLA as a result of its improved bioavailability.

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Metabolic effects of dietary conjugated linoleic acid (CLA) isomers in rats

Cited by 15 publications

References 31 publications

Comparison of the influence of different chemical forms of selenium and the profiles of CLA isomers in the diet on the fatty acid and amino acid contents in the liver and femoral muscles of rats

Comparison of the influence of different chemical forms of selenium and the profiles of CLA isomers in the diet on the fatty acid and amino acid contents in the liver and femoral muscles of rats

Influence of dietary conjugated linoleic acid isomers and selenium on growth, feed efficiency, and liver fatty acid profile in rats

Bioavailability of nanoemulsified conjugated linoleic acid for an antiobesity effect

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