Metabolic Effects of Cobalt Ferrite Nanoparticles on Cervical Carcinoma Cells and Nontumorigenic Keratinocytes

Oliveira, Ana Beatriz Bortolozo de; Moraes, Fábio Rogério de; Cândido, Natália Maria; Sampaio, Isabella; Paula, Alex Silva; Vasconcellos, Adriano de; Silva, Thais Cerqueira; Miller, Alex Henrique; Rahal, Paula; Nery, José Geraldo; Calmon, Marília Freitas

doi:10.1021/acs.jproteome.6b00411

Cited by 23 publications

(16 citation statements)

References 76 publications

(107 reference statements)

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“…This effect is evidence that MNP-PEI internalization interferes with actin-mediated regulatory processes as the actin cytoskeleton is also involved in processes such as proliferation and differentiation 22 . This effect had been noted in previous studies with different cell lines, where iron oxide MNPs and polystyrene particles reduce proliferation 46,47 by interfering with cell metabolism and signalling 48 .…”

Section: Effect Of Mnp-peis On the Cytoskeletonsupporting

confidence: 71%

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

Narayanasamy

Price

Merkhan

et al. 2019

Preprint

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The biocompatibility of surface coated/functionalised magnetic nanoparticles (MNPs) is key to their successful incorporation and application in biological systems. Polyethylene imine (PEI) -coated MNPs provide improved in vitro transfection efficiency compared to conventional chemical methods such as Lipofectamine and cationic polymers, and are also safer than viral transduction. Commercial cell toxicity assays are useful for end-point and high-throughput screening, providing fast results and an overview of cell health. However these assays only take into account cells that have undergone an extreme toxic response leading to cell death. Cell toxicity is a complex process which can be expressed in many forms, through morphological, metabolic, and epigenetic changes. A common indicator of cell stress and toxic response is increased cell adhesion and stress fibre formation. It is important to identify these changes in cells as it may affect downstream results and applications in biomedicine. This study explores the effect of the nanomagnetic transfection agent PEI-coated MNPs (MNP-PEIs) and an external magnetic field on cell behaviour, by studying particle internalization, changes in cellular morphology, and cell adhesion. We found that MNP-PEIs induced cell stress through a dose-dependent increase in cell adhesion via the overexpression of vinculin and formation of actin stress fibres. While the presence of PEI was the main contributor to increased cell stress, free PEI polyplexes induced higher toxicity compared to PEI bound to MNPs. MNPs without PEI coating however did not adversely affect cells suggesting a chemical effect instead of a mechanical one. In addition, genes identified as being associated with actin fibre regulation and cell adhesion, showed significant increases in expression due to the internalization of the MNP-PEI complex. From these results, we identify anomalous cell behaviour, morphology, and gene expression after interaction with MNP-PEIs, as well as a safe dosage to reduce acute cell toxicity.

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Section: Effect Of Mnp-peis On the Cytoskeletonsupporting

confidence: 71%

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

Narayanasamy

Price

Merkhan

et al. 2019

Preprint

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“…Interestingly, in the present study, we could see that the more loading of magnetic field, the higher anti-tumor effects. The phenomenon could possibly be explained by the fact that PTT induced a higher local temperature in tumor cells, thereby increasing the expression of reactive oxygen species (ROS) and heat shock proteins 38 as well as decreasing concentrations of metabolites associated with cell proliferation and tumor growth 39 . Further studies are needed to explore the mechanism of PTT synergy with MCSC scaffolds in inhibiting tumor cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Mesoporous Calcium Sillicate/Chitosan Porous Scaffolds for Enhanced Bone Regeneration and Photothermal-Chemotherapy of Osteosarcoma

Yang

et al. 2018

Sci Rep

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The development of multifunctional biomaterials to repair bone defects after neoplasm removal and inhibit tumor recurrence remained huge clinical challenges. Here, we demonstrate a kind of innovative and multifunctional magnetic mesoporous calcium sillicate/chitosan (MCSC) porous scaffolds, made of M-type ferrite particles (SrFe12O19), mesoporous calcium silicate (CaSiO3) and chitosan (CS), which exert robust anti-tumor and bone regeneration properties. The mesopores in the CaSiO3 microspheres contributed to the drug delivery property, and the SrFe12O19 particles improved photothermal therapy (PTT) conversion efficacy. With the irradiation of NIR laser, doxorubicin (DOX) was rapidly released from the MCSC/DOX scaffolds. In vitro and in vivo tests demonstrated that the MCSC scaffolds possessed the excellent anti-tumor efficacy via the synergetic effect of DOX drug release and hyperthermia ablation. Moreover, BMP-2/Smad/Runx2 pathway was involved in the MCSC scaffolds promoted proliferation and osteogenic differentiation of human bone marrow stromal cells (hBMSCs). Taken together, the MCSC scaffolds have the ability to promote osteogenesis and enhance synergetic photothermal-chemotherapy against osteosarcoma, indicating MCSC scaffolds may have great application potential for bone tumor-related defects.

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“…Therefore, these findings suggest that the TCA cycle may be compromised in cells treated with FLG. Recently, Oliveira et al observed a decrease in creatine/ creatine phosphate in HaCaT cells treated with cobalt ferrite nanoparticles 44 and also an increase in fumarate levels in cells treated with silver nanoparticles. 37 Decreased levels of phosphocreatine can be linked to diverse consequences for the cells, such as increased permeability of the plasma membrane.…”

Section: Metabolomic Cell Profilementioning

confidence: 99%

Differential effects of graphene materials on the metabolism and function of human skin cells

et al. 2018

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Graphene-related materials (GRMs) such as graphene oxide (GO) and few-layer graphene (FLG) are used in multiple biomedical applications; however, there is still insufficient information available regarding their interactions with the main biological barriers such as skin. In this study, we explored the effects of GO and FLG on HaCaTs human skin keratinocytes, using NMR-based metabolomics and fluorescence microscopy to evaluate the global impact of each GRM on cell fate and damage. GO and FLG at low concentrations (5 μg mL-1) induced a differential remodeling of the metabolome, preceded by an increase in the level of radical oxygen species (ROS) and free cytosolic Ca2+. These changes are linked to a concentration-dependent increase in cell death by triggering apoptosis and necrosis, the latter being predominant at higher concentrations of the nanostructures. In addition, both compounds reduce the ability of HaCaT cells to heal wounds. Our results demonstrate that the GO and FLG used in this study, which mainly differ in their oxidation state, slightly trigger differential effects on HaCaTs cells, but with evident outcomes at the cellular and molecular levels. Their behavior as pro-apoptotic/necrotic substances and their ability to inhibit cell migration, even at low doses, should be considered in the development of future applications.

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Metabolic Effects of Cobalt Ferrite Nanoparticles on Cervical Carcinoma Cells and Nontumorigenic Keratinocytes

Cited by 23 publications

References 76 publications

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

Magnetic Mesoporous Calcium Sillicate/Chitosan Porous Scaffolds for Enhanced Bone Regeneration and Photothermal-Chemotherapy of Osteosarcoma

Differential effects of graphene materials on the metabolism and function of human skin cells

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